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Identification of the ‘Haryejosaeng’ mandarin cultivar by multiplex PCR-based SNP genotyping

Development of a PCR-RFLP method for detection of D614G mutation in SARS-CoV-2

In late 2019, an outbreak of respiratory disease named COVID-19 started in the world. To date, thousands of cases of infection are reported worldwide. Most researchers focused on epidemiology and clinical features of COVID-19, and a small part of studies was performed to evaluate the genetic characteristics of this virus. Regarding the high price and low availability of sequencing techniques in developing countries, here we describe a rapid and inexpensive method for the detection of D614G mutation in SARS-CoV-2. Using bioinformatics databases and software,

we designed the PCR-RFLP method for D614G mutation detection. We evaluated 144 SARS-CoV-2 positive samples isolated in six months in Northeastern Iran. Our results showed that the prevalent type is S-D in our isolates, and a small number of isolated belongs to the S-G type. Of 144 samples, 127 (88.2%) samples have belonged to type S-D, and 13 (9%) samples typed S-G. The first S-G type was detected on 2020 June 10. We have little information about the prevalence of D614G mutation, and it seems that the reason is the lack of cheap and fast methods. We hope that this method will provide more information on the prevalence and epidemiology of D614G mutations worldwide.

 

 

Identification of the ‘Haryejosaeng’ mandarin cultivar by multiplex PCR-based SNP genotyping

 

Most satsuma mandarin (Citrus unshiu Marc.) cultivars are difficult to identify in the seedling stage based only on morphological traits. Therefore, simple polymerase chain reaction (PCR)-based single-nucleotide polymorphism (SNP) markers were developed to specifically and rapidly distinguish the ‘Haryejosaeng’ cultivar, which is generally supplied to breeders of other satsuma mandarin cultivars. SNP markers were verified using high-resolution melt (HRM)-specific primers. PCR was performed to distinguish

 

‘Haryejosaeng’ from eight other satsuma mandarin cultivars using six SNP markers (P1-P6) specific for ‘Haryejosaeng’, with one negative control SNP primer pair. The best results were obtained using three SNP markers (P1, P2, and P5). In the multiplex PCR, markers P1, P2, and P5 yielded 165-, 150-, and 526-base pair amplicons, respectively, in ‘Haryejosaeng’, distinguishing it from other satsuma mandarin cultivars.

The selected SNP markers were validated by HRM with HRM-specific primers. The multiplex PCR with P1/P5 and P2/P5 also identified ‘Haryejosaeng’ obtained from a farm growing 17 different cultivars of satsuma mandarin. Specific SNP molecular markers were determined for accurately identifying the ‘Haryejosaeng’ cultivar by multiplex PCR to save the time and costs associated with its supply to breeders of satsuma mandarin.

Clinical Features and Temporal Changes of RT-PCR and Chest CT in COVID-19 Pediatric Patients

Objective: This work aims to investigate the clinical features and the temporal changes of RT-PCR and CT in COVID-19 pediatric patients. Methods: The clinical, RT-PCR, and CT features of 114 COVID-19 pediatric in-patients were retrospectively reviewed from January 21 to March 14, 2020. All patients had chest CT on admission and were identified as positive by pharyngeal swab nucleic acid test. The clinical features were analyzed, as well as the features and the temporal changes of RT-PCR and CT. Results: Fever (62, 54%) and cough (61, 54%) were the most common symptoms.

 

There were 34 (30%) cases of concurrent infections. The most common imaging features on CT were ground-glass opacities (46, 40%) and consolidation (46, 40%). The bilateral lower lobes were the most common pattern of involvement, with 63 cases (55%) involving one to two lobes, and in 32 (28%) cases CT was normal. Throughout the whole duration of COVID-19 in children, the diagnostic positive rate of RT-PCR has been far higher than that of CT (all P < 0.05). For RT-PCR follow-up, reliable negative results were obtained only 7 days after the onset of symptoms. Though lung involvement on chest CT progressed rapidly in several cases, lung involvement in children with COVID-19 is mild, with a median value of 2 on CT score. Conclusions: RT-PCR is more reliable than CT in the initial diagnosis of pediatric patients with COVID-19. On follow-up, reliable negative RT-PCR results are available 7 days after the initial symptoms. The use of CT should be considered for follow-up purposes only if necessary.

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aprofarm

Helicobacter pylori One-Step PCR kit

Oneq-VH347-150D 150T
EUR 1177.2

Helicobacter pylori One-Step PCR kit

Oneq-VH347-50D 50T
EUR 861.6

Helicobacter pylori (H. pylori)

GWB-30366B 1 mg Ask for price

Helicobacter pylori (H. pylori)

GWB-F08333 1 mg Ask for price

Helicobacter pylori

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Helicobacter pylori

Oneq-VH347-OneqVH34750D Oneq-VH347-50D
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Helicobacter pylori

PCR-VH347-PCRVH34748D PCR-VH347-48D
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Helicobacter pylori

PCR-VH347-PCRVH34796D PCR-VH347-96D
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Helicobacter pylori (H. pylori) Protein

abx061476-01ml 0.1 ml
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Helicobacter pylori (H. pylori) Antigen

abx061476-100g 100 µg Ask for price

Helicobacter pylori (H. pylori) Antigen

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Helicobacter pylori (H. pylori) Antigen

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Helicobacter pylori (H. pylori) Antibody

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Helicobacter pylori (H. pylori) Antibody

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Helicobacter pylori (H. pylori) Antibody

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Helicobacter pylori (H. pylori) Antibody

abx415714-200l 200 µl
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Helicobacter pylori IgG

DEHELG0220 96
EUR 88

Helicobacter pylori -HRP

GWB-8536B2 1 ml Ask for price

Helicobacter pylori IgG

GWB-E48E7A 1x96 Assays Ask for price

Helicobacter pylori -FITC

GWB-38B7AC 1 ml Ask for price

Helicobacter Pylori (H. Pylori) IgA ELISA

GWB-BQK20D 96 Well Plate Ask for price

Helicobacter Pylori (H. Pylori) IgG ELISA

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Helicobacter Pylori (H. Pylori) IgM ELISA

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Helicobacter pylori IgG ELISA Kit

DEIA341 96T
EUR 634.8
Description: The Helicobacterpylori IgG Antibody ELISA Test Kit has been designed for the the detectionand the quantitative determination of specific IgG antibodies against Helicobacter pylori in serum and plasma.

Helicobacter pylori IgA ELISA Kit

DEIA342 96T
EUR 634.8
Description: The Helicobacterpylori IgA Antibody ELISA Test Kit has been designed for the the detectionand the quantitative determination of specific IgA antibodies against Helicobacter pylori in serum and plasma.

Helicobacter pylori IgM ELISA Kit

DEIA343 96T
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Description: The Helicobacterpylori IgM Antibody ELISA Test Kit has been designed for the the detectionand the quantitative determination of specific IgM antibodies against Helicobacter pylori in serum and plasma.

Helicobacter pylori IgA ELISA Kit

E4684-100 each
EUR 646.8

Helicobacter pylori IgG ELISA Kit

E4685-100 each
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Helicobacter pylori IgM ELISA Kit

E4686-100 each
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Helicobacter pylori IgA ELISA kit

55R-IB79235 96 wells
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Description: ELISA kit for the detection of Helicobacter pylori IgA in the research laboratory

Helicobacter pylori IgG ELISA kit

55R-IB79236 96 wells
EUR 379.2
Description: ELISA kit for the detection of Helicobacter pylori IgG in the research laboratory

Helicobacter pylori IgM ELISA kit

55R-IB79237 96 wells
EUR 379.2
Description: ELISA kit for the detection of Helicobacter pylori IgM in the research laboratory

Helicobacter pylori IgA ELISA Kit

ESR118A 96 Tests
EUR 409

Helicobacter pylori IgG ELISA Kit

ESR118G 96 Tests
EUR 409

Helicobacter pylori IgM ELISA Kit

ESR118M 96 Tests
EUR 409

Helicobacter pylori protein

30-AH75 1 mg
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Description: Purified native Helicobacter pylori protein

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Description: Purified native Helicobacter pylori protein

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DAG-CL008 1mg
EUR 1326

Helicobacter pylori Antigen

GWB-B10839 1 mg Ask for price

Helicobacter pylori Antigen

PR-BA118-L 1mg
EUR 1009.9
Description: native, Solid medium

Helicobacter pylori Antigen

PR-BA118-S 250µg
EUR 336.1
Description: native, Solid medium

Helicobacter Pylori Antigen

NAT41603-100 0.1
EUR 391.66
Description: Bacteria are cultured on solid medium, harvested, washed and solubilised. The antigen is partially purified by detergent extraction and centrifugation.

Helicobacter Pylori Antigen

NAT41603-500 0.5
EUR 1176.24
Description: Bacteria are cultured on solid medium, harvested, washed and solubilised. The antigen is partially purified by detergent extraction and centrifugation.

Helicobacter pylori Antigen

BA118VS 1mg
EUR 790

Helicobacter Pylori Antigen (H. Pylori) Rapid Test Kit

abx090728-100l 100 µl
EUR 650

Helicobacter Pylori Antigen (H. Pylori) Rapid Test Kit

abx090728-1ml 1 ml
EUR 7200

Helicobacter Pylori Antigen (H. Pylori) Rapid Test Kit

abx090728-200l 200 µl
EUR 2100

Helicobacter pylori Antibody

abx024026-1mg 1 mg
EUR 1212

Helicobacter pylori Antibody

abx021751-1mg 1 mg
EUR 1220.4

Helicobacter pylori Antibody

abx021752-1mg 1 mg
EUR 1220.4

Helicobacter pylori Antibody

abx021753-1mg 1 mg
EUR 1220.4

Helicobacter pylori Antibody

abx021754-1mg 1 mg
EUR 1220.4

Helicobacter pylori Antibody

abx021755-1mg 1 mg
EUR 886.8

Helicobacter pylori Antibody

abx022996-1ml 1 ml
EUR 693.6

Helicobacter pylori antibody

10-H59B 1 mg
EUR 195
Description: Mouse monoclonal Helicobacter pylori antibody

Helicobacter pylori antibody

10-H59C 1 mg
EUR 195
Description: Mouse monoclonal Helicobacter pylori antibody

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10-1268 1 mg
EUR 460
Description: Mouse monoclonal Helicobacter pylori antibody

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10-2672 1 mg
EUR 360
Description: Mouse monoclonal Helicobacter pylori antibody

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10-2673 1 mg
EUR 400
Description: Mouse monoclonal Helicobacter pylori antibody

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EUR 365
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Description: Rabbit polyclonal Helicobacter pylori antibody

Helicobacter pylori Antibody

GWB-433191 0.5 ml Ask for price

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GWB-51DF47 1 ml Ask for price

Helicobacter Pylori Antibody

GWB-53DD97 0.2 mg Ask for price

Helicobacter pylori, Antibody

GWB-546A0E 1 mg Ask for price

Helicobacter pylori, Antibody

GWB-5A24C8 7 ml Ask for price

Helicobacter Pylori Antibody

GWB-61B4E8 6 ml Ask for price

Helicobacter pylori Antibody

GWB-1FB5E0 1 mg Ask for price

Helicobacter pylori Antibody

GWB-27EA55 1 ml Ask for price

Helicobacter pylori, Antibody

GWB-32EE32 1 mg Ask for price

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GWB-344330 0.2 mg Ask for price

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GWB-67195D 1 ml Ask for price

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GWB-B54794 1 ml Ask for price

Helicobacter pylori, Antibody

GWB-BC5C5D 1 ml Ask for price

Helicobacter Pylori Antibody

GWB-C9C4BC 0.2 mg Ask for price

Helicobacter pylori Antibody

R20444-0.1ML 100ul
EUR 409

Helicobacter pylori Antibody

V8786-100UG 100ug
EUR 424.15
Description: The spiral shaped bacterium Helicobacter pylori is strongly associated with inflammation of the stomach and is also implicated in the development of gastric malignancy. H. pylori is known to cause peptic ulcers and chronic gastritis in human. It is associated with duodenal ulcers and may be involved in development of adenocarcinoma and low-grade lymphoma of mucosa associated lymphoid tissue in the stomach. This antibody stains the individual H. pylori bacterium when it presents on the surface of the epithelium or in the cytoplasm of the epithelial cells in biopsy tissue sections from the antrum and body of the stomach.

Helicobacter pylori Antibody

V8786-20UG 20ug
EUR 186.15
Description: The spiral shaped bacterium Helicobacter pylori is strongly associated with inflammation of the stomach and is also implicated in the development of gastric malignancy. H. pylori is known to cause peptic ulcers and chronic gastritis in human. It is associated with duodenal ulcers and may be involved in development of adenocarcinoma and low-grade lymphoma of mucosa associated lymphoid tissue in the stomach. This antibody stains the individual H. pylori bacterium when it presents on the surface of the epithelium or in the cytoplasm of the epithelial cells in biopsy tissue sections from the antrum and body of the stomach.

Helicobacter pylori Antibody

V8786SAF-100UG 100ug
EUR 424.15
Description: The spiral shaped bacterium Helicobacter pylori is strongly associated with inflammation of the stomach and is also implicated in the development of gastric malignancy. H. pylori is known to cause peptic ulcers and chronic gastritis in human. It is associated with duodenal ulcers and may be involved in development of adenocarcinoma and low-grade lymphoma of mucosa associated lymphoid tissue in the stomach. This antibody stains the individual H. pylori bacterium when it presents on the surface of the epithelium or in the cytoplasm of the epithelial cells in biopsy tissue sections from the antrum and body of the stomach.

Helicobacter pylori Antibody

V8827-100UG 100ug
EUR 424.15
Description: The spiral shaped bacteriumHelicobacter pyloriis strongly associated with inflammation of the stomach and is also implicated in the development of gastric malignancy.H. pyloriis known to cause peptic ulcers and chronic gastritis in human. It is associated with duodenal ulcers and may be involved in development of adenocarcinoma and low-grade lymphoma of mucosa associated lymphoid tissue in the stomach.This antibody stains the individual H. pylori bacterium when it presents on the surface of the epithelium or in the cytoplasm of the epithelial cells in biopsy tissue sections from the antrum and body of the stomach.

Helicobacter pylori Antibody

V8827-20UG 20ug
EUR 186.15
Description: The spiral shaped bacteriumHelicobacter pyloriis strongly associated with inflammation of the stomach and is also implicated in the development of gastric malignancy.H. pyloriis known to cause peptic ulcers and chronic gastritis in human. It is associated with duodenal ulcers and may be involved in development of adenocarcinoma and low-grade lymphoma of mucosa associated lymphoid tissue in the stomach.This antibody stains the individual H. pylori bacterium when it presents on the surface of the epithelium or in the cytoplasm of the epithelial cells in biopsy tissue sections from the antrum and body of the stomach.

Helicobacter pylori Antibody

V8827SAF-100UG 100ug
EUR 424.15
Description: The spiral shaped bacteriumHelicobacter pyloriis strongly associated with inflammation of the stomach and is also implicated in the development of gastric malignancy.H. pyloriis known to cause peptic ulcers and chronic gastritis in human. It is associated with duodenal ulcers and may be involved in development of adenocarcinoma and low-grade lymphoma of mucosa associated lymphoid tissue in the stomach.This antibody stains the individual H. pylori bacterium when it presents on the surface of the epithelium or in the cytoplasm of the epithelial cells in biopsy tissue sections from the antrum and body of the stomach.

Helicobacter pylori Antibody

V8329-100UG 100 ug
EUR 424.15
Description: The spiral shaped bacteriumHelicobacter pyloriis strongly associated with inflammation of the stomach and is also implicated in the development of gastric malignancy.H. pyloriis known to cause peptic ulcers and chronic gastritis in human. It is associated with duodenal ulcers and may be involved in development of adenocarcinoma and low-grade lymphoma of mucosa associated lymphoid tissue in the stomach.This antibody stains the individual H. pylori bacterium when it presents on the surface of the epithelium or in the cytoplasm of the epithelial cells in biopsy tissue sections from the antrum and body of the stomach.

Helicobacter pylori Antibody

V8329-20UG 20 ug
EUR 186.15
Description: The spiral shaped bacteriumHelicobacter pyloriis strongly associated with inflammation of the stomach and is also implicated in the development of gastric malignancy.H. pyloriis known to cause peptic ulcers and chronic gastritis in human. It is associated with duodenal ulcers and may be involved in development of adenocarcinoma and low-grade lymphoma of mucosa associated lymphoid tissue in the stomach.This antibody stains the individual H. pylori bacterium when it presents on the surface of the epithelium or in the cytoplasm of the epithelial cells in biopsy tissue sections from the antrum and body of the stomach.

Helicobacter pylori Antibody

V8329SAF-100UG 100 ug
EUR 424.15
Description: The spiral shaped bacteriumHelicobacter pyloriis strongly associated with inflammation of the stomach and is also implicated in the development of gastric malignancy.H. pyloriis known to cause peptic ulcers and chronic gastritis in human. It is associated with duodenal ulcers and may be involved in development of adenocarcinoma and low-grade lymphoma of mucosa associated lymphoid tissue in the stomach.This antibody stains the individual H. pylori bacterium when it presents on the surface of the epithelium or in the cytoplasm of the epithelial cells in biopsy tissue sections from the antrum and body of the stomach.

Helicobacter pylori Antibody

V3089-100UG 100 ug
EUR 424.15
Description: The spiral shaped bacterium Helicobacter pylori is strongly associated with inflammation of the stomach and is also implicated in the development of gastric malignancy. H. pylori is known to cause peptic ulcers and chronic gastritis in human. It is associated with duodenal ulcers and may be involved in development of adenocarcinoma and low-grade lymphoma of mucosa associated lymphoid tissue in the stomach. This antibody stains the individual H. pylori bacterium when it presents on the surface of the epithelium or in the cytoplasm of the epithelial cells in biopsy tissue sections from the antrum and body of the stomach.

Helicobacter pylori Antibody

V3089-20UG 20 ug
EUR 186.15
Description: The spiral shaped bacterium Helicobacter pylori is strongly associated with inflammation of the stomach and is also implicated in the development of gastric malignancy. H. pylori is known to cause peptic ulcers and chronic gastritis in human. It is associated with duodenal ulcers and may be involved in development of adenocarcinoma and low-grade lymphoma of mucosa associated lymphoid tissue in the stomach. This antibody stains the individual H. pylori bacterium when it presents on the surface of the epithelium or in the cytoplasm of the epithelial cells in biopsy tissue sections from the antrum and body of the stomach.

Helicobacter pylori Antibody

V3089SAF-100UG 100 ug
EUR 424.15
Description: The spiral shaped bacterium Helicobacter pylori is strongly associated with inflammation of the stomach and is also implicated in the development of gastric malignancy. H. pylori is known to cause peptic ulcers and chronic gastritis in human. It is associated with duodenal ulcers and may be involved in development of adenocarcinoma and low-grade lymphoma of mucosa associated lymphoid tissue in the stomach. This antibody stains the individual H. pylori bacterium when it presents on the surface of the epithelium or in the cytoplasm of the epithelial cells in biopsy tissue sections from the antrum and body of the stomach.

Helicobacter pylori Antibody

V3103-100UG 100 ug
EUR 424.15
Description: The spiral shaped bacterium Helicobacter pylori is strongly associated with inflammation of the stomach and is also implicated in the development of gastric malignancy. H. pylori is known to cause peptic ulcers and chronic gastritis in human. It is associated with duodenal ulcers and may be involved in development of adenocarcinoma and low-grade lymphoma of mucosa associated lymphoid tissue in the stomach. This antibody stains the individual H. pylori bacterium when it presents on the surface of the epithelium or in the cytoplasm of the epithelial cells in biopsy tissue sections from the antrum and body of the stomach.

Helicobacter pylori Antibody

V3103-20UG 20 ug
EUR 186.15
Description: The spiral shaped bacterium Helicobacter pylori is strongly associated with inflammation of the stomach and is also implicated in the development of gastric malignancy. H. pylori is known to cause peptic ulcers and chronic gastritis in human. It is associated with duodenal ulcers and may be involved in development of adenocarcinoma and low-grade lymphoma of mucosa associated lymphoid tissue in the stomach. This antibody stains the individual H. pylori bacterium when it presents on the surface of the epithelium or in the cytoplasm of the epithelial cells in biopsy tissue sections from the antrum and body of the stomach.

Helicobacter pylori Antibody

V3103SAF-100UG 100 ug
EUR 424.15
Description: The spiral shaped bacterium Helicobacter pylori is strongly associated with inflammation of the stomach and is also implicated in the development of gastric malignancy. H. pylori is known to cause peptic ulcers and chronic gastritis in human. It is associated with duodenal ulcers and may be involved in development of adenocarcinoma and low-grade lymphoma of mucosa associated lymphoid tissue in the stomach. This antibody stains the individual H. pylori bacterium when it presents on the surface of the epithelium or in the cytoplasm of the epithelial cells in biopsy tissue sections from the antrum and body of the stomach.

Helicobacter Pylori Antibody

V2340-100UG 100 ug
EUR 424.15
Description: The spiral shaped bacterium Helicobacter pylori is strongly associated with inflammation of the stomach and is also implicated in the development of gastric malignancy. This bacteria is known to cause peptic ulcers and chronic gastritis in human. It is associated with duodenal ulcers and may be involved in development of adenocarcinoma and low-grade lymphoma of mucosa associated lymphoid tissue in the stomach. This antibody stains the individual Helicobacter pylori bacterium when it presents on the surface of the epithelium or in the cytoplasm of the epithelial cells in biopsy tissue sections from the antrum and body of the stomach.

Helicobacter Pylori Antibody

V2340-20UG 20 ug
EUR 186.15
Description: The spiral shaped bacterium Helicobacter pylori is strongly associated with inflammation of the stomach and is also implicated in the development of gastric malignancy. This bacteria is known to cause peptic ulcers and chronic gastritis in human. It is associated with duodenal ulcers and may be involved in development of adenocarcinoma and low-grade lymphoma of mucosa associated lymphoid tissue in the stomach. This antibody stains the individual Helicobacter pylori bacterium when it presents on the surface of the epithelium or in the cytoplasm of the epithelial cells in biopsy tissue sections from the antrum and body of the stomach.

Helicobacter Pylori Antibody

V2340SAF-100UG 100 ug
EUR 424.15
Description: The spiral shaped bacterium Helicobacter pylori is strongly associated with inflammation of the stomach and is also implicated in the development of gastric malignancy. This bacteria is known to cause peptic ulcers and chronic gastritis in human. It is associated with duodenal ulcers and may be involved in development of adenocarcinoma and low-grade lymphoma of mucosa associated lymphoid tissue in the stomach. This antibody stains the individual Helicobacter pylori bacterium when it presents on the surface of the epithelium or in the cytoplasm of the epithelial cells in biopsy tissue sections from the antrum and body of the stomach.

Helicobacter pylori Antibody

abx022996-400l 400 µl Ask for price

Helicobacter pylori Antibody

abx022996-80l 80 µl
EUR 1012.5

Helicobacter pylori Antibody

abx021751-400l 400 µl Ask for price

Helicobacter pylori Antibody

abx021751-80l 80 µl
EUR 1200

Helicobacter pylori Antibody

abx021752-400l 400 µl Ask for price

Helicobacter pylori Antibody

abx021752-80l 80 µl
EUR 1200

Helicobacter pylori Antibody

abx021753-400l 400 µl Ask for price

Helicobacter pylori Antibody

abx021753-80l 80 µl
EUR 1200

Helicobacter pylori Antibody

abx021754-400l 400 µl Ask for price

Helicobacter pylori Antibody

abx021754-80l 80 µl
EUR 1200

Helicobacter pylori Antibody

abx021755-400l 400 µl Ask for price

Helicobacter pylori Antibody

abx021755-80l 80 µl
EUR 1150

Helicobacter pylori IgA plus

GWB-280643 1x96 Assays Ask for price

Helicobacter pylori IgG plus

GWB-EC00D0 1x96 Assays Ask for price

Helicobacter pylori IgG ELISA

GWB-4B9691 ELISA_Kits Ask for price

Helicobacter pylori IgA ELISA

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Helicobacter pylori IgM ELISA

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Helicobacter pylori Agar - EACH

MED1338 EACH
EUR 136.35

Helicobacter pylori (H. pylori) (flagellin) Antibody

abx023967-1mg 1 mg
EUR 794.4

Helicobacter pylori (H. pylori) (flagellin) Antibody

abx023967-400l 400 µl Ask for price

Helicobacter pylori (H. pylori) (flagellin) Antibody

abx023967-80l 80 µl
EUR 725

Rat Helicobacter pylori IgA ELISA kit

E02H0261-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Rat Helicobacter pylori IgA in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Helicobacter pylori IgA ELISA kit

E02H0261-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Rat Helicobacter pylori IgA in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Helicobacter pylori IgA ELISA kit

E02H0261-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Rat Helicobacter pylori IgA in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Helicobacter pylori IgG ELISA kit

E02H0268-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Rat Helicobacter pylori IgG in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Quantification of Ammonia Oxidizing Bacterial Abundances in Environmental Samples by Quantitative-PCR

Quantitative-PCR (qPCR) enables the quantification of specific DNA targets, such as functional or phylogenetic marker genes associated with environmental samples. During each qPCR cycle, the number of copies of a gene (or region) of interest in DNA samples is determined in real time using a fluorescence-based label and compared to a standard serial dilution.
Here, we describe a qPCR method to quantify the ammonia oxidizing bacteria involved in the first step of nitrification, using the amoA gene as a proxy of their abundance. The preparation of the standards from environmental samples and qPCR is presented in detail for specifically quantifying microbial abundance in environmental samples such as soil.

Peptide Nucleic Acid (PNA) Clamps to Reduce Co-amplification of Plant DNA During PCR Amplification of 16S rRNA Genes from Endophytic Bacteria

High-throughput sequencing of universal bacterial 16S rRNA gene (16S rDNA) amplicons is a routine method for characterizing bacterial diversity in a range of environments. For eukaryotic host-associated communities, however, plastid and mitochondrial genes are often co-amplified with, and greatly outnumber, bacterial 16S rDNA.
This makes it difficult to obtain sufficient numbers of target 16S rDNA sequences to characterize the diversity of endophytic bacterial communities. This chapter describes a method that improves the amplification of bacterial 16S rDNA from plant tissues by using a peptide nucleic acid (PNA) PCR clamp. The PNA clamp selectively binds to a targeted region of the plant genome and inhibits its amplification during PCR. PNA clamps are especially useful for characterizing bacterial communities on plant tissues with lower levels of microbial colonization such as the root tips and leaves.

Retrospective Post-mortem SARS-CoV-2 RT-PCR of Autopsies with COVID-19-Suggestive Pathology Supports the Absence of Lethal Community Spread in Basel, Switzerland, before February 2020

Introduction: Coronavirus disease 2019 (COVID-19) caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has rapidly spread around the world. While the first case was recorded in Hubei in December 2019, the extent of early community spread in Central Europe before this period is unknown. A high proportion of asymptomatic cases and undocumented infections, high transmissibility, and phylogenetic genomic diversity have engendered the controversial possibility of early international community spread of SARS-CoV-2 before its emergence in China.
Methods: To assess the early presence of lethal COVID-19 in Switzerland, we retrospectively performed an analysis of deaths at University Hospital Basel between October 2019 and February 2020 (n = 310), comparing the incidence of clinical causes of death with March 2020 (n = 72), the month during which the first lethal COVID-19 cases in Basel were reported. Trends of COVID-19-suggestive sequelae, such as bronchopneumonia with organization, acute respiratory distress syndrome (ARDS), or pulmonary embolisms (PE) were evaluated.
In cases where autopsy was performed (n = 71), analogous analyses were conducted on the cause of death and pulmonary histological findings. Eight cases with a COVID-19-suggestive clinical history and histopathology between October 2019 and February 2020, and 3 cases before October 2019, were selected for SARS-CoV-2 RT-PCR.

HCV Seroconversion Panel Donor# 68896 (6 X 1 mL)

HCV10009 6 X 1 mL
EUR 1101

HCV Seroconversion Panel Donor# 65470 (7 X 1 mL)

HCV10010 7 X 1 mL
EUR 1101

HCV Seroconversion Panel Donor# 66666 (13 X 1 mL)

HCV10011 13 X 1 mL
EUR 1101

HCV Seroconversion Panel Donor# 66747 (13 X 1 mL)

HCV10012 13 X 1 mL
EUR 1101

HCV Seroconversion Panel Donor# 67356 (10 X 1 mL)

HCV10013 10 X 1 mL
EUR 1101

HCV Seroconversion Panel Donor# 68157 (4 X 1 mL)

HCV10014 4 X 1 mL
EUR 1101

HCV Seroconversion Panel Donor# 68628 (12 X 1 mL)

HCV10016 12 X 1 mL
EUR 1101

HCV Seroconversion Panel Donor# 65064 (16 X 1 mL)

HCV10017 16 X 1 mL
EUR 1764

HCV Seroconversion Panel Donor# 69142 (8 X 1 mL)

HCV10019 8 X 1 mL
EUR 1101

HCV Seroconversion Panel Donor# 68744 (13 X 1 mL)

HCV10020 13 X 1 mL
EUR 1101

HCV Seroconversion Panel Donor# 68470 (14 X 1 mL)

HCV10021 14 X 1 mL
EUR 1101

HCV Seroconversion Panel Donor# 68450 (11 X 1 mL)

HCV10022 11 X 1 mL
EUR 1101

HCV Seroconversion Panel Donor# 67505 (21 X 1 mL)

HCV10023 21 X 1 mL
EUR 1101

HCV Seroconversion Panel Donor# 66719 (8 X 1 mL)

HCV10024 8 X 1 mL
EUR 1101

HCV Seroconversion Panel Donor# 66801 (11 X 1 mL)

HCV10025 11 X 1 mL
EUR 1101

HCV Seroconversion Panel Donor# 67391 (12 X 1 mL)

HCV10026 12 X 1 mL
EUR 1764

HCV Seroconversion Panel Donor# 66751 (8 X 1 mL)

HCV10027 8 X 1 mL
EUR 1101

HCV Seroconversion Panel Donor# 67116 (7 X 1 mL)

HCV10028 7 X 1 mL
EUR 1101

HCV Seroconversion Panel Donor# 67539 (14 X 1 mL)

HCV10029 16 X 1 mL
EUR 1101

HCV Seroconversion Panel Donor# 65977 (8 X 1 mL)

HCV10030 8 X 1 mL
EUR 1101

HCV Seroconversion Panel Donor# 66113 (16 X 1 mL)

HCV10032 16 X 1 mL
EUR 1101

HCV Seroconversion Panel Donor# 68629 (10 X 1 mL)

HCV10033 10 X 1 mL
EUR 1101

HCV Seroconversion Panel Donor# 68223 (13 X 1 mL)

HCV10036 13 X 1 mL
EUR 1101

HCV Seroconversion Panel Donor# 65643 (10 X 1 mL)

HCV10043 10 X 1 mL
EUR 1101

HCV Seroconversion Panel Donor# 65982 (4 X 1 mL)

HCV10044 4 X 1 mL
EUR 1101

HCV Seroconversion Panel Donor# 66074 (5 X 1 mL)

HCV10045 5 X 1 mL
EUR 1101

HCV Seroconversion Panel Donor# 67766 (15 X 1 mL)

HCV10050 15 X 1 mL
EUR 1101

HCV Seroconversion Panel Donor# 67787 (14 X 1 mL)

HCV10051 14 X 1 mL
EUR 1101

HCV Seroconversion Panel Donor# 67861 (12 X 1 mL)

HCV10052 12 X 1 mL
EUR 1101

HCV Seroconversion Panel Donor# 420245049 (7 X 1 mL)

HCV10057 7 X 1 mL
EUR 1764

HCV Seroconversion Panel Donor# 420299823 (7 X 1 mL)

HCV10058 7 X 1 mL
EUR 1858.37
Description: Please contact Gentaur in order to receive the datasheet of the product.

HCV Seroconversion Panel Donor# 490104584 (8 X 1 mL)

HCV10062 8 X 1 mL
EUR 1764

HCV Seroconversion Panel Donor# 90163052 (7 X 1 mL)

HCV10071 7 X 1 mL
EUR 1764

HCV Seroconversion Panel Donor# 72237 (9 X 1 mL)

HCV10165 9 X 1 mL
EUR 1764

HCV Seroconversion Panel Donor# 70225 (5 X 1 mL)

HCV10185 5 X 1 mL
EUR 1764

HCV Seroconversion Panel Donor# 61025 (5 X 1 mL)

HCV6217 5 X 1 mL
EUR 1035

HCV Seroconversion Panel Donor# 61251 (8 X 1 mL)

HCV6218 8 X 1 mL
EUR 1035

HCV Seroconversion Panel Donor# 60749 (14 X 1 mL)

HCV6219 14 X 1 mL
EUR 1035

HCV Seroconversion Panel Donor# 63792 (6 X 1 mL)

HCV6220 6 X 1 mL
EUR 1035

HCV Seroconversion Panel Donor# 62258 (8 X 1 mL)

HCV6221 8 X 1 mL
EUR 1035

HCV Seroconversion Panel Donor# 62286 (8 X 1 mL)

HCV6222 8 X 1 mL
EUR 1764

HCV Seroconversion Panel Donor# 62507 (22 X 1 mL)

HCV6223 22 X 1 mL
EUR 1035

HCV Seroconversion Panel Donor# 62680 (6 X 1 mL)

HCV6224 6 X 1 mL
EUR 1764

HCV Seroconversion Panel Donor# 62999 (19 X 1 mL)

HCV6225 19 X 1 mL
EUR 1764

HCV Seroconversion Panel Donor# 63135 (12 X 1 mL)

HCV6226 12 X 1 mL
EUR 1764

HCV Seroconversion Panel Donor# 63318 (7 X 1 mL)

HCV6227 7 X 1 mL
EUR 1764

HCV Seroconversion Panel Donor# 62886 (12 X 1 mL)

HCV6228 12 X 1 mL
EUR 1764

HCV Seroconversion Panel Donor# 62804 (8 X 1 mL)

HCV6229 8 X 1 mL
EUR 1764

HCV Seroconversion Panel Donor# 63625 (8 X 1 mL)

HCV9041 8 X 1 mL
EUR 1764

HCV Seroconversion Panel Donor# 63792 (6 X 1 mL)

HCV9042 6 X 1 mL
EUR 1035

HCV Seroconversion Panel Donor# 63869 (6 X 1 mL)

HCV9043 6 X 1 mL
EUR 1035

HCV Seroconversion Panel Donor# 63911 (6 X 1 mL)

HCV9044 6 X 1 mL
EUR 1764

HCV Seroconversion Panel Donor# 64150 (8 X 1 mL)

HCV9045 8 X 1 mL
EUR 1764

HCV Seroconversion Panel Donor# 64254 (5 X 1 mL)

HCV9046 5 X 1 mL
EUR 1764

HCV Seroconversion Panel Donor# 64273 (10 X 1 mL)

HCV9047 10 X 1 mL
EUR 1764

HCV Seroconversion Panel Donor# 64437 (8 X 1 mL)

HCV9048 8 X 1 mL
EUR 1035

HCV Seroconversion Panel Donor# 64766 (5 X 1 mL)

HCV9049 5 X 1 mL
EUR 1035

HCV Seroconversion Panel Donor# 66158 (13 X 1 mL)

HCV9050 13 X 1 mL
EUR 1764

HCV Seroconversion Panel Donor# 66626 (8 X 1 mL)

HCV9054 8 X 1 mL
EUR 1764

HCV Seroconversion Panel Donor# 66732 (11 X 1 mL)

HCV9055 11 X 1 mL
EUR 1764

HCV Seroconversion Panel Donor# 66861 (11 X 1 mL)

HCV9057 11 X 1 mL
EUR 1764

HCV Seroconversion Panel Donor# 66647 (5 X 1 mL)

HCV9058 5 X 1 mL
EUR 1764

HCV Seroconversion Panel Donor# 1038851 (5 X 1 mL)

HCV9095 5 X 1 mL
EUR 1764

HCV Seroconversion Panel Donor# 22708D (5 X 1 mL)

HCV9094 5 X 1 mL
EUR 1764

HCV Pre/Post Seroconversion Panel Donor# 66822 (5 X 1 mL)

HCV10235 5 X 1 mL
EUR 545

HIV Seroconversion Panel Donor# 65404 (7 X mL)

HIV9013 7 X mL
EUR 1764

EBV Seroconversion Panel Donor# 283843421 (14 X 1 mL)

EBV9097 14 X 1 mL
EUR 1764

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