aprofarm

Identification of the ‘Haryejosaeng’ mandarin cultivar by multiplex PCR-based SNP genotyping

Development of a PCR-RFLP method for detection of D614G mutation in SARS-CoV-2

In late 2019, an outbreak of respiratory disease named COVID-19 started in the world. To date, thousands of cases of infection are reported worldwide. Most researchers focused on epidemiology and clinical features of COVID-19, and a small part of studies was performed to evaluate the genetic characteristics of this virus. Regarding the high price and low availability of sequencing techniques in developing countries, here we describe a rapid and inexpensive method for the detection of D614G mutation in SARS-CoV-2. Using bioinformatics databases and software,

we designed the PCR-RFLP method for D614G mutation detection. We evaluated 144 SARS-CoV-2 positive samples isolated in six months in Northeastern Iran. Our results showed that the prevalent type is S-D in our isolates, and a small number of isolated belongs to the S-G type. Of 144 samples, 127 (88.2%) samples have belonged to type S-D, and 13 (9%) samples typed S-G. The first S-G type was detected on 2020 June 10. We have little information about the prevalence of D614G mutation, and it seems that the reason is the lack of cheap and fast methods. We hope that this method will provide more information on the prevalence and epidemiology of D614G mutations worldwide.

 

 

Identification of the ‘Haryejosaeng’ mandarin cultivar by multiplex PCR-based SNP genotyping

 

Most satsuma mandarin (Citrus unshiu Marc.) cultivars are difficult to identify in the seedling stage based only on morphological traits. Therefore, simple polymerase chain reaction (PCR)-based single-nucleotide polymorphism (SNP) markers were developed to specifically and rapidly distinguish the ‘Haryejosaeng’ cultivar, which is generally supplied to breeders of other satsuma mandarin cultivars. SNP markers were verified using high-resolution melt (HRM)-specific primers. PCR was performed to distinguish

 

‘Haryejosaeng’ from eight other satsuma mandarin cultivars using six SNP markers (P1-P6) specific for ‘Haryejosaeng’, with one negative control SNP primer pair. The best results were obtained using three SNP markers (P1, P2, and P5). In the multiplex PCR, markers P1, P2, and P5 yielded 165-, 150-, and 526-base pair amplicons, respectively, in ‘Haryejosaeng’, distinguishing it from other satsuma mandarin cultivars.

The selected SNP markers were validated by HRM with HRM-specific primers. The multiplex PCR with P1/P5 and P2/P5 also identified ‘Haryejosaeng’ obtained from a farm growing 17 different cultivars of satsuma mandarin. Specific SNP molecular markers were determined for accurately identifying the ‘Haryejosaeng’ cultivar by multiplex PCR to save the time and costs associated with its supply to breeders of satsuma mandarin.

Clinical Features and Temporal Changes of RT-PCR and Chest CT in COVID-19 Pediatric Patients

Objective: This work aims to investigate the clinical features and the temporal changes of RT-PCR and CT in COVID-19 pediatric patients. Methods: The clinical, RT-PCR, and CT features of 114 COVID-19 pediatric in-patients were retrospectively reviewed from January 21 to March 14, 2020. All patients had chest CT on admission and were identified as positive by pharyngeal swab nucleic acid test. The clinical features were analyzed, as well as the features and the temporal changes of RT-PCR and CT. Results: Fever (62, 54%) and cough (61, 54%) were the most common symptoms.

 

There were 34 (30%) cases of concurrent infections. The most common imaging features on CT were ground-glass opacities (46, 40%) and consolidation (46, 40%). The bilateral lower lobes were the most common pattern of involvement, with 63 cases (55%) involving one to two lobes, and in 32 (28%) cases CT was normal. Throughout the whole duration of COVID-19 in children, the diagnostic positive rate of RT-PCR has been far higher than that of CT (all P < 0.05). For RT-PCR follow-up, reliable negative results were obtained only 7 days after the onset of symptoms. Though lung involvement on chest CT progressed rapidly in several cases, lung involvement in children with COVID-19 is mild, with a median value of 2 on CT score. Conclusions: RT-PCR is more reliable than CT in the initial diagnosis of pediatric patients with COVID-19. On follow-up, reliable negative RT-PCR results are available 7 days after the initial symptoms. The use of CT should be considered for follow-up purposes only if necessary.

aprofarm
aprofarm

lyophilised real-time PCR Master Mix for dual labeled probes

M-PCR-156S 192 reactions x 20 µl
EUR 225.3
Description: lyophilised real-time PCR Master Mix for dual labeled probes

Helicobacter pylori RT PCR kit

RTq-VH347-100D 100T
EUR 860.4

Helicobacter pylori RT PCR kit

RTq-VH347-150D 150T
EUR 969.6

Helicobacter pylori RT PCR kit

RTq-VH347-50D 50T
EUR 717.6

Mouse Helicobacter PCR kit

PCR-V346-48D 50T
EUR 543.6

Mouse Helicobacter PCR kit

PCR-V346-96D 100T
EUR 686.4

Helicobacter spp. PCR kit

PCR-H520-48D 50T
EUR 543.6

Helicobacter spp. PCR kit

PCR-H520-96D 100T
EUR 686.4

Helicobacter pylori One-Step PCR kit

Oneq-VH347-100D 100T
EUR 1039.2

Helicobacter pylori One-Step PCR kit

Oneq-VH347-150D 150T
EUR 1177.2

Helicobacter pylori One-Step PCR kit

Oneq-VH347-50D 50T
EUR 861.6

TurboQ Real-time PCR Machine with Laptop

MOL2700 EACH
EUR 30805.08

lyophilised real-time PCR Master Mix with SYBR® Green fluorescent DNA stain

M-PCR-173L 960 reactions x 20 µl
EUR 789.36
Description: lyophilised real-time PCR Master Mix with SYBR® Green fluorescent DNA stain

lyophilised real-time PCR Master Mix with SYBR® Green fluorescent DNA stain

M-PCR-173S 192 reactions x 20 µl
EUR 197.34
Description: lyophilised real-time PCR Master Mix with SYBR® Green fluorescent DNA stain

hsa-mir-521 Real-time RT-PCR Detection Kit

20-abx097600
  • EUR 661.20
  • EUR 944.40
  • EUR 477.60
  • 100 rxns
  • 200 rxns
  • 50 rxns

dsGreen for Real-Time PCR, 100×, 5 mL

71010 5 mL
EUR 750

dsGreen for Real-Time PCR, 100×, 2 mL

61010 2 mL
EUR 435.6

dsGreen for Real-Time PCR, 100×, 1 mL

41010 1 mL
EUR 298.8

dsGreen for Real-Time PCR, 100×, 10 mL

81010 10 mL
EUR 1042.8

PMA Real-Time PCR Bacterial Viability Kit - Staphylococcus aureus (mecA)

31036 1kit
EUR 478.8
Description: Minimum order quantity: 1 unit of 1kit

PMA Real-Time PCR Bacterial Viability Kit - E. coli (uidA)

31050 1kit
EUR 513.6
Description: Minimum order quantity: 1 unit of 1kit

PMA Real-Time PCR Bacterial Viability Kit - Listeria monocytogenes (hly)

31051 1kit
EUR 478.8
Description: Minimum order quantity: 1 unit of 1kit

PMA Real-Time PCR Bacterial Viability Kit - Legionella pneumophila (mip)

31053 1kit
EUR 513.6
Description: Minimum order quantity: 1 unit of 1kit

ReadiView® Blue Real-Time PCR Visualization Dye *200X*

17300 500 Tests
EUR 97

qPCR MultiplexMasterMaster mix for multiplex real-time PCR** shipped ice packs - must be shipped via overnight service

M-PCR-321L 10 x 1,25 ml
EUR 1438.81
Description: qPCR MultiplexMasterMaster mix for multiplex real-time PCR** shipped ice packs - must be shipped via overnight service

qPCR MultiplexMasterMaster mix for multiplex real-time PCR** shipped ice packs - must be shipped via overnight service

M-PCR-321S 2 x 1,25 ml
EUR 359.77
Description: qPCR MultiplexMasterMaster mix for multiplex real-time PCR** shipped ice packs - must be shipped via overnight service

PMA Real-Time PCR Bacterial Viability Kit - Salmonella enterica (invA) PMAxx

31033-X 1kit
EUR 513.6
Description: Minimum order quantity: 1 unit of 1kit

PMA Real-Time PCR Bacterial Viability Kit - E. coli (uidA) PMAxx

31050-X 1kit
EUR 513.6
Description: Minimum order quantity: 1 unit of 1kit

PMA Real-Time PCR Bacterial Viability Kit - Listeria monocytogenes (hly) PMAxx

31051-X 1kit
EUR 513.6
Description: Minimum order quantity: 1 unit of 1kit

Novel Coronavirus COVID-19 (2019-nCoV) Real Time RT-PCR Kit

RR-0478-02 25 tests/kit
EUR 1189.2
Description: Novel Coronavirus (2019-nCoV) Real Time RT-PCR Kit is used for the qualitative detection of a novel coronavirus, which was identified in 2019 at Wuhan City, Hubei Province, China, in upper respiratory tract specimens (nasopharyngeal extracts, deep cough sputum, etc.) and lower respiratory tract specimens (alveoli irrigation fluid, etc.) by real time PCR systems.

Axygen Polyester ultra clear sealing film for real time PCR

AXY2152 PK100
EUR 336.3

qPCR MultiplexMaster lowROXMaster mix for multiplex real-time PCR** shipped ice packs - must be shipped via overnight service

M-PCR-322L 10 x 1,25 ml
EUR 1438.81
Description: qPCR MultiplexMaster lowROXMaster mix for multiplex real-time PCR** shipped ice packs - must be shipped via overnight service

qPCR MultiplexMaster lowROXMaster mix for multiplex real-time PCR** shipped ice packs - must be shipped via overnight service

M-PCR-322S 2 x 1,25 ml
EUR 359.77
Description: qPCR MultiplexMaster lowROXMaster mix for multiplex real-time PCR** shipped ice packs - must be shipped via overnight service

qPCR MultiplexMaster highROXMaster mix for multiplex real-time PCR** shipped ice packs - must be shipped via overnight service

M-PCR-323L 10 x 1,25 ml
EUR 1438.81
Description: qPCR MultiplexMaster highROXMaster mix for multiplex real-time PCR** shipped ice packs - must be shipped via overnight service

qPCR MultiplexMaster highROXMaster mix for multiplex real-time PCR** shipped ice packs - must be shipped via overnight service

M-PCR-323S 2 x 1,25 ml
EUR 359.77
Description: qPCR MultiplexMaster highROXMaster mix for multiplex real-time PCR** shipped ice packs - must be shipped via overnight service

Human Lung Cancer PCR Primer Library

HLUCPL-I 1 set
EUR 657.6

Human Schwann Cell PCR Primer Library

HSCH-I 1 set
EUR 657.6

Helicobacter pylori (H. pylori) Protein

abx061476-01ml 0.1 ml
EUR 661.2

Helicobacter pylori (H. pylori) Antibody

abx415714-1ml 1 ml
EUR 727.2

Human Real-time PCR DNA Quantitation after QPCR DNA Damage Analysis Kit

DD2H 1 Kit
EUR 516

Mouse Real-time PCR DNA Quantitation after QPCR DNA Damage Analysis Kit

DD2M 1 Kit
EUR 516

Rat Real-time PCR DNA Quantitation after QPCR DNA Damage Analysis Kit

DD2R 1 Kit
EUR 516

PMA Real-Time PCR Bacterial Viability Kit - E. coli O157:H7 (Z3276)

31037 1kit
EUR 513.6
Description: Minimum order quantity: 1 unit of 1kit

hsa-mir-521 Real-Time RT-PCR Detection and U6 Calibration Kit

20-abx097601
  • EUR 878.40
  • EUR 1245.60
  • EUR 627.60
  • 100 rxns
  • 200 rxns
  • 50 rxns

Helicobacter pylori protein

DAG-CL008 1mg
EUR 1326

Helicobacter pylori antibody

20-HR76 1 mg
EUR 139.2
Description: Rabbit polyclonal Helicobacter pylori antibody

Helicobacter pylori protein

30-AH75 1 mg
EUR 306
Description: Purified native Helicobacter pylori protein

Helicobacter pylori protein

30-AH77 1 mg
EUR 567.6
Description: Purified Helicobacter pylori protein

Helicobacter pylori protein

30-AH78 1 mg
EUR 307.2
Description: Purified native Helicobacter pylori protein

Helicobacter pylori antibody

10-1268 1 mg
EUR 954
Description: Mouse monoclonal Helicobacter pylori antibody

Helicobacter pylori antibody

10-2672 1 mg
EUR 502.8
Description: Mouse monoclonal Helicobacter pylori antibody

Helicobacter pylori antibody

10-2673 1 mg
EUR 502.8
Description: Mouse monoclonal Helicobacter pylori antibody

Helicobacter pylori antibody

10-2723 1 mg
EUR 508.8
Description: Mouse monoclonal Helicobacter pylori antibody

Helicobacter pylori antibody

10-H59B 1 mg
EUR 289.2
Description: Mouse monoclonal Helicobacter pylori antibody

Helicobacter pylori antibody

10-H59C 1 mg
EUR 289.2
Description: Mouse monoclonal Helicobacter pylori antibody

Helicobacter pylori Antibody

abx021751-1mg 1 mg
EUR 1220.4

Helicobacter pylori Antibody

abx021752-1mg 1 mg
EUR 1220.4

Helicobacter pylori Antibody

abx021753-1mg 1 mg
EUR 1220.4

Helicobacter pylori Antibody

abx021754-1mg 1 mg
EUR 1220.4

Helicobacter pylori Antibody

abx021755-1mg 1 mg
EUR 886.8

Helicobacter pylori Antibody

abx022996-1ml 1 ml
EUR 693.6

Helicobacter pylori Antibody

abx024026-1mg 1 mg
EUR 1212

Helicobacter pylori Agar

MED1338 EACH
EUR 104.88

Helicobacter pylori Antibody

V8329-100UG 100 ug
EUR 499
Description: The spiral shaped bacteriumHelicobacter pyloriis strongly associated with inflammation of the stomach and is also implicated in the development of gastric malignancy.H. pyloriis known to cause peptic ulcers and chronic gastritis in human. It is associated with duodenal ulcers and may be involved in development of adenocarcinoma and low-grade lymphoma of mucosa associated lymphoid tissue in the stomach.This antibody stains the individual H. pylori bacterium when it presents on the surface of the epithelium or in the cytoplasm of the epithelial cells in biopsy tissue sections from the antrum and body of the stomach.

Helicobacter pylori Antibody

V8329-20UG 20 ug
EUR 219
Description: The spiral shaped bacteriumHelicobacter pyloriis strongly associated with inflammation of the stomach and is also implicated in the development of gastric malignancy.H. pyloriis known to cause peptic ulcers and chronic gastritis in human. It is associated with duodenal ulcers and may be involved in development of adenocarcinoma and low-grade lymphoma of mucosa associated lymphoid tissue in the stomach.This antibody stains the individual H. pylori bacterium when it presents on the surface of the epithelium or in the cytoplasm of the epithelial cells in biopsy tissue sections from the antrum and body of the stomach.

Helicobacter pylori Antibody

V8329SAF-100UG 100 ug
EUR 499
Description: The spiral shaped bacteriumHelicobacter pyloriis strongly associated with inflammation of the stomach and is also implicated in the development of gastric malignancy.H. pyloriis known to cause peptic ulcers and chronic gastritis in human. It is associated with duodenal ulcers and may be involved in development of adenocarcinoma and low-grade lymphoma of mucosa associated lymphoid tissue in the stomach.This antibody stains the individual H. pylori bacterium when it presents on the surface of the epithelium or in the cytoplasm of the epithelial cells in biopsy tissue sections from the antrum and body of the stomach.

Helicobacter Pylori Antibody

V2340-100UG 100 ug
EUR 499
Description: The spiral shaped bacterium Helicobacter pylori is strongly associated with inflammation of the stomach and is also implicated in the development of gastric malignancy. This bacteria is known to cause peptic ulcers and chronic gastritis in human. It is associated with duodenal ulcers and may be involved in development of adenocarcinoma and low-grade lymphoma of mucosa associated lymphoid tissue in the stomach. This antibody stains the individual Helicobacter pylori bacterium when it presents on the surface of the epithelium or in the cytoplasm of the epithelial cells in biopsy tissue sections from the antrum and body of the stomach.

Helicobacter Pylori Antibody

V2340-20UG 20 ug
EUR 219
Description: The spiral shaped bacterium Helicobacter pylori is strongly associated with inflammation of the stomach and is also implicated in the development of gastric malignancy. This bacteria is known to cause peptic ulcers and chronic gastritis in human. It is associated with duodenal ulcers and may be involved in development of adenocarcinoma and low-grade lymphoma of mucosa associated lymphoid tissue in the stomach. This antibody stains the individual Helicobacter pylori bacterium when it presents on the surface of the epithelium or in the cytoplasm of the epithelial cells in biopsy tissue sections from the antrum and body of the stomach.

Helicobacter Pylori Antibody

V2340SAF-100UG 100 ug
EUR 499
Description: The spiral shaped bacterium Helicobacter pylori is strongly associated with inflammation of the stomach and is also implicated in the development of gastric malignancy. This bacteria is known to cause peptic ulcers and chronic gastritis in human. It is associated with duodenal ulcers and may be involved in development of adenocarcinoma and low-grade lymphoma of mucosa associated lymphoid tissue in the stomach. This antibody stains the individual Helicobacter pylori bacterium when it presents on the surface of the epithelium or in the cytoplasm of the epithelial cells in biopsy tissue sections from the antrum and body of the stomach.

Helicobacter pylori Antibody

V3089-100UG 100 ug
EUR 499
Description: The spiral shaped bacterium Helicobacter pylori is strongly associated with inflammation of the stomach and is also implicated in the development of gastric malignancy. H. pylori is known to cause peptic ulcers and chronic gastritis in human. It is associated with duodenal ulcers and may be involved in development of adenocarcinoma and low-grade lymphoma of mucosa associated lymphoid tissue in the stomach. This antibody stains the individual H. pylori bacterium when it presents on the surface of the epithelium or in the cytoplasm of the epithelial cells in biopsy tissue sections from the antrum and body of the stomach.

Helicobacter pylori Antibody

V3089-20UG 20 ug
EUR 219
Description: The spiral shaped bacterium Helicobacter pylori is strongly associated with inflammation of the stomach and is also implicated in the development of gastric malignancy. H. pylori is known to cause peptic ulcers and chronic gastritis in human. It is associated with duodenal ulcers and may be involved in development of adenocarcinoma and low-grade lymphoma of mucosa associated lymphoid tissue in the stomach. This antibody stains the individual H. pylori bacterium when it presents on the surface of the epithelium or in the cytoplasm of the epithelial cells in biopsy tissue sections from the antrum and body of the stomach.

Helicobacter pylori Antibody

V3089SAF-100UG 100 ug
EUR 499
Description: The spiral shaped bacterium Helicobacter pylori is strongly associated with inflammation of the stomach and is also implicated in the development of gastric malignancy. H. pylori is known to cause peptic ulcers and chronic gastritis in human. It is associated with duodenal ulcers and may be involved in development of adenocarcinoma and low-grade lymphoma of mucosa associated lymphoid tissue in the stomach. This antibody stains the individual H. pylori bacterium when it presents on the surface of the epithelium or in the cytoplasm of the epithelial cells in biopsy tissue sections from the antrum and body of the stomach.

Helicobacter pylori Antibody

V3103-100UG 100 ug
EUR 499
Description: The spiral shaped bacterium Helicobacter pylori is strongly associated with inflammation of the stomach and is also implicated in the development of gastric malignancy. H. pylori is known to cause peptic ulcers and chronic gastritis in human. It is associated with duodenal ulcers and may be involved in development of adenocarcinoma and low-grade lymphoma of mucosa associated lymphoid tissue in the stomach. This antibody stains the individual H. pylori bacterium when it presents on the surface of the epithelium or in the cytoplasm of the epithelial cells in biopsy tissue sections from the antrum and body of the stomach.

Helicobacter pylori Antibody

V3103-20UG 20 ug
EUR 219
Description: The spiral shaped bacterium Helicobacter pylori is strongly associated with inflammation of the stomach and is also implicated in the development of gastric malignancy. H. pylori is known to cause peptic ulcers and chronic gastritis in human. It is associated with duodenal ulcers and may be involved in development of adenocarcinoma and low-grade lymphoma of mucosa associated lymphoid tissue in the stomach. This antibody stains the individual H. pylori bacterium when it presents on the surface of the epithelium or in the cytoplasm of the epithelial cells in biopsy tissue sections from the antrum and body of the stomach.

Helicobacter pylori Antibody

V3103SAF-100UG 100 ug
EUR 499
Description: The spiral shaped bacterium Helicobacter pylori is strongly associated with inflammation of the stomach and is also implicated in the development of gastric malignancy. H. pylori is known to cause peptic ulcers and chronic gastritis in human. It is associated with duodenal ulcers and may be involved in development of adenocarcinoma and low-grade lymphoma of mucosa associated lymphoid tissue in the stomach. This antibody stains the individual H. pylori bacterium when it presents on the surface of the epithelium or in the cytoplasm of the epithelial cells in biopsy tissue sections from the antrum and body of the stomach.

Helicobacter pylori Antibody

V8786-100UG 100ug
EUR 499
Description: The spiral shaped bacterium Helicobacter pylori is strongly associated with inflammation of the stomach and is also implicated in the development of gastric malignancy. H. pylori is known to cause peptic ulcers and chronic gastritis in human. It is associated with duodenal ulcers and may be involved in development of adenocarcinoma and low-grade lymphoma of mucosa associated lymphoid tissue in the stomach. This antibody stains the individual H. pylori bacterium when it presents on the surface of the epithelium or in the cytoplasm of the epithelial cells in biopsy tissue sections from the antrum and body of the stomach.

Helicobacter pylori Antibody

V8786-20UG 20ug
EUR 219
Description: The spiral shaped bacterium Helicobacter pylori is strongly associated with inflammation of the stomach and is also implicated in the development of gastric malignancy. H. pylori is known to cause peptic ulcers and chronic gastritis in human. It is associated with duodenal ulcers and may be involved in development of adenocarcinoma and low-grade lymphoma of mucosa associated lymphoid tissue in the stomach. This antibody stains the individual H. pylori bacterium when it presents on the surface of the epithelium or in the cytoplasm of the epithelial cells in biopsy tissue sections from the antrum and body of the stomach.

Helicobacter pylori Antibody

V8786SAF-100UG 100ug
EUR 499
Description: The spiral shaped bacterium Helicobacter pylori is strongly associated with inflammation of the stomach and is also implicated in the development of gastric malignancy. H. pylori is known to cause peptic ulcers and chronic gastritis in human. It is associated with duodenal ulcers and may be involved in development of adenocarcinoma and low-grade lymphoma of mucosa associated lymphoid tissue in the stomach. This antibody stains the individual H. pylori bacterium when it presents on the surface of the epithelium or in the cytoplasm of the epithelial cells in biopsy tissue sections from the antrum and body of the stomach.

Helicobacter pylori Antibody

V8827-100UG 100ug
EUR 499
Description: The spiral shaped bacteriumHelicobacter pyloriis strongly associated with inflammation of the stomach and is also implicated in the development of gastric malignancy.H. pyloriis known to cause peptic ulcers and chronic gastritis in human. It is associated with duodenal ulcers and may be involved in development of adenocarcinoma and low-grade lymphoma of mucosa associated lymphoid tissue in the stomach.This antibody stains the individual H. pylori bacterium when it presents on the surface of the epithelium or in the cytoplasm of the epithelial cells in biopsy tissue sections from the antrum and body of the stomach.

Helicobacter pylori Antibody

V8827-20UG 20ug
EUR 219
Description: The spiral shaped bacteriumHelicobacter pyloriis strongly associated with inflammation of the stomach and is also implicated in the development of gastric malignancy.H. pyloriis known to cause peptic ulcers and chronic gastritis in human. It is associated with duodenal ulcers and may be involved in development of adenocarcinoma and low-grade lymphoma of mucosa associated lymphoid tissue in the stomach.This antibody stains the individual H. pylori bacterium when it presents on the surface of the epithelium or in the cytoplasm of the epithelial cells in biopsy tissue sections from the antrum and body of the stomach.

Helicobacter pylori Antibody

V8827SAF-100UG 100ug
EUR 499
Description: The spiral shaped bacteriumHelicobacter pyloriis strongly associated with inflammation of the stomach and is also implicated in the development of gastric malignancy.H. pyloriis known to cause peptic ulcers and chronic gastritis in human. It is associated with duodenal ulcers and may be involved in development of adenocarcinoma and low-grade lymphoma of mucosa associated lymphoid tissue in the stomach.This antibody stains the individual H. pylori bacterium when it presents on the surface of the epithelium or in the cytoplasm of the epithelial cells in biopsy tissue sections from the antrum and body of the stomach.

PCR Mycoplasma Detection Kit

M034-Kit Kit
EUR 319.2

qPCR plate-foil universal (for Real-time PCR, Light Cycler 480)

A26979 1x100 pcs
EUR 223.2

Eco 48 Real Time PCR system including software and loading dock

MOL6348 EACH
EUR 19520.22

Eztime Real - time PCRPremix 2x,Taqman

FYT105-100P 100 Preps 1.25 ml Ask for price

Helicobacter pylori IgG ELISA Kit

DEIA341 96T
EUR 634.8
Description: The Helicobacterpylori IgG Antibody ELISA Test Kit has been designed for the the detectionand the quantitative determination of specific IgG antibodies against Helicobacter pylori in serum and plasma.

Helicobacter pylori IgA ELISA Kit

DEIA342 96T
EUR 634.8
Description: The Helicobacterpylori IgA Antibody ELISA Test Kit has been designed for the the detectionand the quantitative determination of specific IgA antibodies against Helicobacter pylori in serum and plasma.

Helicobacter pylori IgM ELISA Kit

DEIA343 96T
EUR 674.4
Description: The Helicobacterpylori IgM Antibody ELISA Test Kit has been designed for the the detectionand the quantitative determination of specific IgM antibodies against Helicobacter pylori in serum and plasma.

Helicobacter pylori IgA ELISA kit

55R-IB79235 96 wells
EUR 379.2
Description: ELISA kit for the detection of Helicobacter pylori IgA in the research laboratory

Helicobacter pylori IgG ELISA kit

55R-IB79236 96 wells
EUR 379.2
Description: ELISA kit for the detection of Helicobacter pylori IgG in the research laboratory

Helicobacter pylori IgM ELISA kit

55R-IB79237 96 wells
EUR 379.2
Description: ELISA kit for the detection of Helicobacter pylori IgM in the research laboratory

Helicobacter pylori IgA ELISA Kit

E4684-100 each
EUR 646.8

Helicobacter pylori IgG ELISA Kit

E4685-100 each
EUR 646.8

Helicobacter pylori IgM ELISA Kit

E4686-100 each
EUR 646.8

PMA Real-Time PCR Bacterial Viability Kit - E. coli O157:H7 (Z3276) PMAxx

31037-X 1kit
EUR 513.6
Description: Minimum order quantity: 1 unit of 1kit

Helicobacter pylori (H. pylori) (flagellin) Antibody

abx023967-1mg 1 mg
EUR 794.4

SYBR® Green Fluorescent DNA StainDNA intercalation dye for real-time PCR analysis** shipped ice packs - must be shipped via overnight service

M-PCR-378 500 µl
EUR 63.63
Description: SYBR® Green Fluorescent DNA StainDNA intercalation dye for real-time PCR analysis** shipped ice packs - must be shipped via overnight service

Helicobacter pylori antibody (FITC)

60-H76 1 ml
EUR 468
Description: Rabbit polyclonal Helicobacter pylori antibody (FITC) conjugated

Helicobacter pylori antibody (HRP)

60-H77 1 ml
EUR 490.8
Description: Rabbit polyclonal Helicobacter pylori antibody (HRP) conjugated

Helicobacter pylori CagA Antibody

abx021756-1mg 1 mg
EUR 878.4

Helicobacter pylori Antibody (FITC)

abx022997-1ml 1 ml
EUR 661.2

Helicobacter pylori Antibody (HRP)

abx022998-1ml 1 ml
EUR 777.6

Anti-Helicobacter Pylori antibody

STJ160107 1 mL C
EUR 1129.2

Anti-Helicobacter pylori antibody

STJ16101332 1 mL
EUR 570

Anti-Helicobacter pylori antibody

STJ16101468 1 mL
EUR 570

Helicobacter Pylori (Control Slides)

TCS0011-100 100 Slides
EUR 847.2

Helicobacter Pylori (Control Slides)

TCS0011-25 25 Slides
EUR 278.4

Helicobacter Pylori (Control Slides)

TCS0011-5 5 Slides
EUR 117.6

Helicobacter Pylori Antigen Extract

VAng-Lsx3187-1mL 1 mL
EUR 1057.2
Description: Liquid Helicobacter Pylori Antigen Extract from Gastric biopsy.

Purified Helicobacter Pylori Antigen

VAng-Lsx3188-100g 100 µg
EUR 1688.4
Description: Purified Helicobacter Pylori Antigen from E. coli.

Helicobacter pylori Selective Supplement

MED1674 PK10
EUR 66.12

Helicobacter pylori Catalase Antibody

V9170-100UG 100ug
EUR 499
Description: The spiral shaped bacterium Helicobacter pylori is strongly associated with inflammation of the stomach and is also implicated in the development of gastric malignancy. H. pylori is known to cause peptic ulcers and chronic gastritis in human. It is associated with duodenal ulcers and may be involved in development of adenocarcinoma and low-grade lymphoma of mucosa associated lymphoid tissue in the stomach. This antibody stains the individual H. pylori bacterium when it presents on the surface of the epithelium or in the cytoplasm of the epithelial cells in biopsy tissue sections from the antrum and body of the stomach.

Helicobacter pylori Catalase Antibody

V9170-20UG 20ug
EUR 219
Description: The spiral shaped bacterium Helicobacter pylori is strongly associated with inflammation of the stomach and is also implicated in the development of gastric malignancy. H. pylori is known to cause peptic ulcers and chronic gastritis in human. It is associated with duodenal ulcers and may be involved in development of adenocarcinoma and low-grade lymphoma of mucosa associated lymphoid tissue in the stomach. This antibody stains the individual H. pylori bacterium when it presents on the surface of the epithelium or in the cytoplasm of the epithelial cells in biopsy tissue sections from the antrum and body of the stomach.

Helicobacter pylori Catalase Antibody

V9170SAF-100UG 100ug
EUR 499
Description: The spiral shaped bacterium Helicobacter pylori is strongly associated with inflammation of the stomach and is also implicated in the development of gastric malignancy. H. pylori is known to cause peptic ulcers and chronic gastritis in human. It is associated with duodenal ulcers and may be involved in development of adenocarcinoma and low-grade lymphoma of mucosa associated lymphoid tissue in the stomach. This antibody stains the individual H. pylori bacterium when it presents on the surface of the epithelium or in the cytoplasm of the epithelial cells in biopsy tissue sections from the antrum and body of the stomach.

0,1ML THIN WALL TUBES 8 PER STRIP. CLEAR & REAL TIME STRIP CAPS

PCR-0108-LP-RT-C 125/pk
EUR 850.8
Description: PCR Plates & Tubes; PCR Strip Tubes and Strip Caps - Axygen

0,1ML THIN WALL TUBES 8 PER STRIP. WHITE & REAL TIME STRIP CAPS

PCR-0108-LP-RT-W 125/pk
EUR 924
Description: PCR Plates & Tubes; PCR Strip Tubes and Strip Caps - Axygen

Eztime Real - time PCRPremix 2x,SYBR Green

FYT103-100P 100 Preps 1.25 ml Ask for price

Eztime Real - time PCRPremix 2x,SYBR Green

FYT103-400P 400 Preps 1.25 ml x 4 Ask for price

Eztime Real - time PCRPremix 2x,SYBRGreen , ROX

FYT104-100P 100 Preps 1.25 ml Ask for price

Eztime Real - time PCRPremix 2x,SYBRGreen , ROX

FYT104-400P 400 Preps 1.25 ml x 4 Ask for price

Eztime Real - time PCRPremix 2x,Taqman, ROX

FYT105-400P 400 Preps 1.25 ml x 4 Ask for price

Quantification of Ammonia Oxidizing Bacterial Abundances in Environmental Samples by Quantitative-PCR

Quantitative-PCR (qPCR) enables the quantification of specific DNA targets, such as functional or phylogenetic marker genes associated with environmental samples. During each qPCR cycle, the number of copies of a gene (or region) of interest in DNA samples is determined in real time using a fluorescence-based label and compared to a standard serial dilution.
Here, we describe a qPCR method to quantify the ammonia oxidizing bacteria involved in the first step of nitrification, using the amoA gene as a proxy of their abundance. The preparation of the standards from environmental samples and qPCR is presented in detail for specifically quantifying microbial abundance in environmental samples such as soil.

Peptide Nucleic Acid (PNA) Clamps to Reduce Co-amplification of Plant DNA During PCR Amplification of 16S rRNA Genes from Endophytic Bacteria

High-throughput sequencing of universal bacterial 16S rRNA gene (16S rDNA) amplicons is a routine method for characterizing bacterial diversity in a range of environments. For eukaryotic host-associated communities, however, plastid and mitochondrial genes are often co-amplified with, and greatly outnumber, bacterial 16S rDNA.
This makes it difficult to obtain sufficient numbers of target 16S rDNA sequences to characterize the diversity of endophytic bacterial communities. This chapter describes a method that improves the amplification of bacterial 16S rDNA from plant tissues by using a peptide nucleic acid (PNA) PCR clamp. The PNA clamp selectively binds to a targeted region of the plant genome and inhibits its amplification during PCR. PNA clamps are especially useful for characterizing bacterial communities on plant tissues with lower levels of microbial colonization such as the root tips and leaves.

Retrospective Post-mortem SARS-CoV-2 RT-PCR of Autopsies with COVID-19-Suggestive Pathology Supports the Absence of Lethal Community Spread in Basel, Switzerland, before February 2020

Introduction: Coronavirus disease 2019 (COVID-19) caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has rapidly spread around the world. While the first case was recorded in Hubei in December 2019, the extent of early community spread in Central Europe before this period is unknown. A high proportion of asymptomatic cases and undocumented infections, high transmissibility, and phylogenetic genomic diversity have engendered the controversial possibility of early international community spread of SARS-CoV-2 before its emergence in China.
Methods: To assess the early presence of lethal COVID-19 in Switzerland, we retrospectively performed an analysis of deaths at University Hospital Basel between October 2019 and February 2020 (n = 310), comparing the incidence of clinical causes of death with March 2020 (n = 72), the month during which the first lethal COVID-19 cases in Basel were reported. Trends of COVID-19-suggestive sequelae, such as bronchopneumonia with organization, acute respiratory distress syndrome (ARDS), or pulmonary embolisms (PE) were evaluated.
In cases where autopsy was performed (n = 71), analogous analyses were conducted on the cause of death and pulmonary histological findings. Eight cases with a COVID-19-suggestive clinical history and histopathology between October 2019 and February 2020, and 3 cases before October 2019, were selected for SARS-CoV-2 RT-PCR.

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Positive control tissue section for each individua

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