aprofarm

Identification of the ‘Haryejosaeng’ mandarin cultivar by multiplex PCR-based SNP genotyping

Development of a PCR-RFLP method for detection of D614G mutation in SARS-CoV-2

In late 2019, an outbreak of respiratory disease named COVID-19 started in the world. To date, thousands of cases of infection are reported worldwide. Most researchers focused on epidemiology and clinical features of COVID-19, and a small part of studies was performed to evaluate the genetic characteristics of this virus. Regarding the high price and low availability of sequencing techniques in developing countries, here we describe a rapid and inexpensive method for the detection of D614G mutation in SARS-CoV-2. Using bioinformatics databases and software,

we designed the PCR-RFLP method for D614G mutation detection. We evaluated 144 SARS-CoV-2 positive samples isolated in six months in Northeastern Iran. Our results showed that the prevalent type is S-D in our isolates, and a small number of isolated belongs to the S-G type. Of 144 samples, 127 (88.2%) samples have belonged to type S-D, and 13 (9%) samples typed S-G. The first S-G type was detected on 2020 June 10. We have little information about the prevalence of D614G mutation, and it seems that the reason is the lack of cheap and fast methods. We hope that this method will provide more information on the prevalence and epidemiology of D614G mutations worldwide.

 

 

Identification of the ‘Haryejosaeng’ mandarin cultivar by multiplex PCR-based SNP genotyping

 

Most satsuma mandarin (Citrus unshiu Marc.) cultivars are difficult to identify in the seedling stage based only on morphological traits. Therefore, simple polymerase chain reaction (PCR)-based single-nucleotide polymorphism (SNP) markers were developed to specifically and rapidly distinguish the ‘Haryejosaeng’ cultivar, which is generally supplied to breeders of other satsuma mandarin cultivars. SNP markers were verified using high-resolution melt (HRM)-specific primers. PCR was performed to distinguish

 

‘Haryejosaeng’ from eight other satsuma mandarin cultivars using six SNP markers (P1-P6) specific for ‘Haryejosaeng’, with one negative control SNP primer pair. The best results were obtained using three SNP markers (P1, P2, and P5). In the multiplex PCR, markers P1, P2, and P5 yielded 165-, 150-, and 526-base pair amplicons, respectively, in ‘Haryejosaeng’, distinguishing it from other satsuma mandarin cultivars.

The selected SNP markers were validated by HRM with HRM-specific primers. The multiplex PCR with P1/P5 and P2/P5 also identified ‘Haryejosaeng’ obtained from a farm growing 17 different cultivars of satsuma mandarin. Specific SNP molecular markers were determined for accurately identifying the ‘Haryejosaeng’ cultivar by multiplex PCR to save the time and costs associated with its supply to breeders of satsuma mandarin.

Clinical Features and Temporal Changes of RT-PCR and Chest CT in COVID-19 Pediatric Patients

Objective: This work aims to investigate the clinical features and the temporal changes of RT-PCR and CT in COVID-19 pediatric patients. Methods: The clinical, RT-PCR, and CT features of 114 COVID-19 pediatric in-patients were retrospectively reviewed from January 21 to March 14, 2020. All patients had chest CT on admission and were identified as positive by pharyngeal swab nucleic acid test. The clinical features were analyzed, as well as the features and the temporal changes of RT-PCR and CT. Results: Fever (62, 54%) and cough (61, 54%) were the most common symptoms.

 

There were 34 (30%) cases of concurrent infections. The most common imaging features on CT were ground-glass opacities (46, 40%) and consolidation (46, 40%). The bilateral lower lobes were the most common pattern of involvement, with 63 cases (55%) involving one to two lobes, and in 32 (28%) cases CT was normal. Throughout the whole duration of COVID-19 in children, the diagnostic positive rate of RT-PCR has been far higher than that of CT (all P < 0.05). For RT-PCR follow-up, reliable negative results were obtained only 7 days after the onset of symptoms. Though lung involvement on chest CT progressed rapidly in several cases, lung involvement in children with COVID-19 is mild, with a median value of 2 on CT score. Conclusions: RT-PCR is more reliable than CT in the initial diagnosis of pediatric patients with COVID-19. On follow-up, reliable negative RT-PCR results are available 7 days after the initial symptoms. The use of CT should be considered for follow-up purposes only if necessary.

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aprofarm

Monkeypox Virus Real Time PCR Kit

ZD-0076-02 25 tests/kit Ask for price
Description: Monkeypox virus is the virus that causes the disease monkeypox in both humans and animals. Monkeypox virus is an Orthopoxvirus, a genus of the family Poxviridae that contains other viral species that target mammals. The virus is mainly found in tropical rainforest regions of central and West Africa. The primary route of infection is thought to be contact with the infected animals or their bodily fluids.The genome is not segmented and contains a single molecule of linear double-stranded DNA, 185000 nucleotides long.The Monkeypox Virus real time PCR Kit contains a specific ready-to-use system for the detection of the Monkeypox Virusthrough polymerase chain reaction (PCR) in the real-time PCR system. The master contains reagents and enzymes for the specific amplification of theMonkeypox VirusDNA. Fluorescence is emitted and measured by the real time systems ́ optical unit during the PCR. The detection of amplified Monkeypox Virus DNA fragment is performed in fluorimeter channelFAM with the fluorescent quencher BHQ1. DNA extraction buffer is available in the kit and serum or lesion exudate samples are used for the extraction of the DNA. In addition, the kit contains a system to identify possible PCR inhibition by measuring the HEX/VIC/JOE fluorescence of the internal control (IC). An external positive control defined as 1×107copies/ml is supplied which allow the determination of the gene load.

Helicobacter pylori RT PCR kit

RTq-VH347-100D 100T
EUR 717

Helicobacter pylori RT PCR kit

RTq-VH347-150D 150T
EUR 808

Helicobacter pylori RT PCR kit

RTq-VH347-50D 50T
EUR 598

Helicobacter spp. PCR kit

PCR-H520-48D 50T
EUR 453

Helicobacter spp. PCR kit

PCR-H520-96D 100T
EUR 572

Mouse Helicobacter PCR kit

PCR-V346-48D 50T
EUR 453

Mouse Helicobacter PCR kit

PCR-V346-96D 100T
EUR 572

Helicobacter pylori One-Step PCR kit

Oneq-VH347-100D 100T
EUR 866

Helicobacter pylori One-Step PCR kit

Oneq-VH347-150D 150T
EUR 981

Helicobacter pylori One-Step PCR kit

Oneq-VH347-50D 50T
EUR 718

hsa-mir-521 Real-time RT-PCR Detection Kit

20-abx097600
  • EUR 551.00
  • EUR 787.00
  • EUR 398.00
  • 100 rxns
  • 200 rxns
  • 50 rxns

dsGreen for Real-Time PCR, 100×, 1 mL

41010 1 mL
EUR 249

dsGreen for Real-Time PCR, 100×, 1.5 mL

51010 1.5 mL
EUR 331

dsGreen for Real-Time PCR, 100×, 2 mL

61010 2 mL
EUR 363

dsGreen for Real-Time PCR, 100×, 100 uL

11010 100 uL
EUR 59

dsGreen for Real-Time PCR, 100×, 5 mL

71010 5 mL
EUR 625

dsGreen for Real-Time PCR, 100×, 10 mL

81010 10 mL
EUR 869

dsGreen for Real-Time PCR, 100×, 50 mL

91010 50 mL
EUR 3169

PMA Real-Time PCR Bacterial Viability Kit - Salmonella enterica (invA)

31033 1kit
EUR 428
Description: Minimum order quantity: 1 unit of 1kit

PMA Real-Time PCR Bacterial Viability Kit - Mycobacterium tuberculosis (groEL2)

31034 1kit
EUR 399
Description: Minimum order quantity: 1 unit of 1kit

PMA Real-Time PCR Bacterial Viability Kit - Staphylococcus aureus (nuc)

31035 1kit
EUR 399
Description: Minimum order quantity: 1 unit of 1kit

PMA Real-Time PCR Bacterial Viability Kit - Staphylococcus aureus (mecA)

31036 1kit
EUR 399
Description: Minimum order quantity: 1 unit of 1kit

PMA Real-Time PCR Bacterial Viability Kit - E. coli (uidA)

31050 1kit
EUR 428
Description: Minimum order quantity: 1 unit of 1kit

PMA Real-Time PCR Bacterial Viability Kit - Listeria monocytogenes (hly)

31051 1kit
EUR 399
Description: Minimum order quantity: 1 unit of 1kit

PMA Real-Time PCR Bacterial Viability Kit - Legionella pneumophila (mip)

31053 1kit
EUR 428
Description: Minimum order quantity: 1 unit of 1kit

Novel Coronavirus COVID-19 (2019-nCoV) Real Time RT-PCR Kit

RR-0478-02 25 tests/kit
EUR 991
Description: Novel Coronavirus (2019-nCoV) Real Time RT-PCR Kit is used for the qualitative detection of a novel coronavirus, which was identified in 2019 at Wuhan City, Hubei Province, China, in upper respiratory tract specimens (nasopharyngeal extracts, deep cough sputum, etc.) and lower respiratory tract specimens (alveoli irrigation fluid, etc.) by real time PCR systems.

PMA Real-Time PCR Bacterial Viability Kit - Salmonella enterica (invA) PMAxx

31033-X 1kit
EUR 428
Description: Minimum order quantity: 1 unit of 1kit

PMA Real-Time PCR Bacterial Viability Kit - E. coli (uidA) PMAxx

31050-X 1kit
EUR 428
Description: Minimum order quantity: 1 unit of 1kit

PMA Real-Time PCR Bacterial Viability Kit - Listeria monocytogenes (hly) PMAxx

31051-X 1kit
EUR 428
Description: Minimum order quantity: 1 unit of 1kit

Helicobacter pylori (H. pylori) Protein

abx061476-01ml 0.1 ml
EUR 551

Helicobacter pylori (H. pylori) Antibody

abx415714-1ml 1 ml
EUR 606

Helicobacter pylori protein

DAG-CL008 1mg
EUR 1105

Helicobacter pylori Antibody

abx024026-1mg 1 mg
EUR 1010

Helicobacter pylori Antibody

abx021751-1mg 1 mg
EUR 1017

Helicobacter pylori Antibody

abx021752-1mg 1 mg
EUR 1017

Helicobacter pylori Antibody

abx021753-1mg 1 mg
EUR 1017

Helicobacter pylori Antibody

abx021754-1mg 1 mg
EUR 1017

Helicobacter pylori Antibody

abx021755-1mg 1 mg
EUR 739

Helicobacter pylori Antibody

abx022996-1ml 1 ml
EUR 578

Helicobacter pylori antibody

20-HR76 1 mg
EUR 116
Description: Rabbit polyclonal Helicobacter pylori antibody

Helicobacter pylori antibody

10-1268 1 mg
EUR 795
Description: Mouse monoclonal Helicobacter pylori antibody

Helicobacter pylori antibody

10-2672 1 mg
EUR 419
Description: Mouse monoclonal Helicobacter pylori antibody

Helicobacter pylori antibody

10-2673 1 mg
EUR 419
Description: Mouse monoclonal Helicobacter pylori antibody

Helicobacter pylori antibody

10-2723 1 mg
EUR 424
Description: Mouse monoclonal Helicobacter pylori antibody

Helicobacter pylori antibody

10-H59B 1 mg
EUR 241
Description: Mouse monoclonal Helicobacter pylori antibody

Helicobacter pylori antibody

10-H59C 1 mg
EUR 241
Description: Mouse monoclonal Helicobacter pylori antibody

Helicobacter pylori protein

30-AH75 1 mg
EUR 255
Description: Purified native Helicobacter pylori protein

Helicobacter pylori protein

30-AH77 1 mg
EUR 473
Description: Purified Helicobacter pylori protein

Helicobacter pylori protein

30-AH78 1 mg
EUR 256
Description: Purified native Helicobacter pylori protein

Human Real-time PCR DNA Quantitation after QPCR DNA Damage Analysis Kit

DD2H 1 Kit
EUR 430

Mouse Real-time PCR DNA Quantitation after QPCR DNA Damage Analysis Kit

DD2M 1 Kit
EUR 430

Rat Real-time PCR DNA Quantitation after QPCR DNA Damage Analysis Kit

DD2R 1 Kit
EUR 430

PMA Real-Time PCR Bacterial Viability Kit - E. coli O157:H7 (Z3276)

31037 1kit
EUR 428
Description: Minimum order quantity: 1 unit of 1kit

hsa-mir-521 Real-Time RT-PCR Detection and U6 Calibration Kit

20-abx097601
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns

qPCR plate-foil universal (for Real-time PCR, Light Cycler 480)

A26979 1x100 pcs
EUR 186

Helicobacter pylori IgG ELISA Kit

DEIA341 96T
EUR 529
Description: The Helicobacterpylori IgG Antibody ELISA Test Kit has been designed for the the detectionand the quantitative determination of specific IgG antibodies against Helicobacter pylori in serum and plasma.

Helicobacter pylori IgA ELISA Kit

DEIA342 96T
EUR 529
Description: The Helicobacterpylori IgA Antibody ELISA Test Kit has been designed for the the detectionand the quantitative determination of specific IgA antibodies against Helicobacter pylori in serum and plasma.

Helicobacter pylori IgM ELISA Kit

DEIA343 96T
EUR 562
Description: The Helicobacterpylori IgM Antibody ELISA Test Kit has been designed for the the detectionand the quantitative determination of specific IgM antibodies against Helicobacter pylori in serum and plasma.

Helicobacter pylori IgA ELISA kit

55R-IB79235 96 wells
EUR 316
Description: ELISA kit for the detection of Helicobacter pylori IgA in the research laboratory

Helicobacter pylori IgG ELISA kit

55R-IB79236 96 wells
EUR 316
Description: ELISA kit for the detection of Helicobacter pylori IgG in the research laboratory

Helicobacter pylori IgM ELISA kit

55R-IB79237 96 wells
EUR 316
Description: ELISA kit for the detection of Helicobacter pylori IgM in the research laboratory

Helicobacter pylori IgA ELISA Kit

E4684-100
EUR 539

Helicobacter pylori IgG ELISA Kit

E4685-100
EUR 539

Helicobacter pylori IgM ELISA Kit

E4686-100
EUR 539

Helicobacter pylori (H. pylori) (flagellin) Antibody

abx023967-1mg 1 mg
EUR 662

PCR Mycoplasma Detection Kit

M034-Kit Kit
EUR 266

Eztime Real - time PCRPremix 2x,Taqman

FYT105-100P 100 Preps 1.25 ml Ask for price

Anti-Helicobacter Pylori antibody

STJ160107 1 mL C
EUR 941

Anti-Helicobacter pylori antibody

STJ16101332 1 mL
EUR 475

Anti-Helicobacter pylori antibody

STJ16101468 1 mL
EUR 475

Helicobacter Pylori Antigen Extract

VAng-Lsx3187-1mL 1 mL
EUR 881
Description: Liquid Helicobacter Pylori Antigen Extract from Gastric biopsy.

Purified Helicobacter Pylori Antigen

VAng-Lsx3188-100g 100 µg
EUR 1407
Description: Purified Helicobacter Pylori Antigen from E. coli.

Helicobacter pylori CagA Antibody

abx021756-1mg 1 mg
EUR 732

Helicobacter pylori Antibody (FITC)

abx022997-1ml 1 ml
EUR 551

Helicobacter pylori Antibody (HRP)

abx022998-1ml 1 ml
EUR 648

Helicobacter Pylori (Control Slides)

TCS0011-100 100 Slides
EUR 706

Helicobacter Pylori (Control Slides)

TCS0011-25 25 Slides
EUR 232

Helicobacter Pylori (Control Slides)

TCS0011-5 5 Slides
EUR 98

Helicobacter pylori antibody (FITC)

60-H76 1 ml
EUR 390
Description: Rabbit polyclonal Helicobacter pylori antibody (FITC) conjugated

Helicobacter pylori antibody (HRP)

60-H77 1 ml
EUR 409
Description: Rabbit polyclonal Helicobacter pylori antibody (HRP) conjugated

PMA Real-Time PCR Bacterial Viability Kit - E. coli O157:H7 (Z3276) PMAxx

31037-X 1kit
EUR 428
Description: Minimum order quantity: 1 unit of 1kit

Human Lung Cancer PCR Primer Library

HLUCPL-I 1 set
EUR 548

Human Schwann Cell PCR Primer Library

HSCH-I 1 set
EUR 548

Mouse Helicobacter pylori IgG ELISA Kit

ELA-E1387m 96 Tests
EUR 865

Rat Helicobacter pylori IgA ELISA kit

E02H0261-192T 192 tests
EUR 1270
Description: A competitive ELISA for quantitative measurement of Rat Helicobacter pylori IgA in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Helicobacter pylori IgA ELISA kit

E02H0261-48 1 plate of 48 wells
EUR 520
Description: A competitive ELISA for quantitative measurement of Rat Helicobacter pylori IgA in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Helicobacter pylori IgA ELISA kit

E02H0261-96 1 plate of 96 wells
EUR 685
Description: A competitive ELISA for quantitative measurement of Rat Helicobacter pylori IgA in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Helicobacter pylori CagA ELISA kit

E02H0262-192T 192 tests
EUR 1270
Description: A competitive ELISA for quantitative measurement of Rat Helicobacter pylori CagA in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Helicobacter pylori CagA ELISA kit

E02H0262-48 1 plate of 48 wells
EUR 520
Description: A competitive ELISA for quantitative measurement of Rat Helicobacter pylori CagA in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Helicobacter pylori CagA ELISA kit

E02H0262-96 1 plate of 96 wells
EUR 685
Description: A competitive ELISA for quantitative measurement of Rat Helicobacter pylori CagA in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Helicobacter pylori antibody ELISA kit

E02H0263-192T 192 tests
EUR 1270
Description: A competitive ELISA for quantitative measurement of Rat Helicobacter pylori antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Helicobacter pylori antibody ELISA kit

E02H0263-48 1 plate of 48 wells
EUR 520
Description: A competitive ELISA for quantitative measurement of Rat Helicobacter pylori antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Helicobacter pylori antibody ELISA kit

E02H0263-96 1 plate of 96 wells
EUR 685
Description: A competitive ELISA for quantitative measurement of Rat Helicobacter pylori antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Helicobacter pylori Hsp60 ELISA kit

E02H0267-192T 192 tests
EUR 1270
Description: A sandwich ELISA for quantitative measurement of Rat Helicobacter pylori Hsp60 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Helicobacter pylori Hsp60 ELISA kit

E02H0267-48 1 plate of 48 wells
EUR 520
Description: A sandwich ELISA for quantitative measurement of Rat Helicobacter pylori Hsp60 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Helicobacter pylori Hsp60 ELISA kit

E02H0267-96 1 plate of 96 wells
EUR 685
Description: A sandwich ELISA for quantitative measurement of Rat Helicobacter pylori Hsp60 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Helicobacter pylori IgG ELISA kit

E02H0268-192T 192 tests
EUR 1270
Description: A competitive ELISA for quantitative measurement of Rat Helicobacter pylori IgG in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Helicobacter pylori IgG ELISA kit

E02H0268-48 1 plate of 48 wells
EUR 520
Description: A competitive ELISA for quantitative measurement of Rat Helicobacter pylori IgG in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Helicobacter pylori IgG ELISA kit

E02H0268-96 1 plate of 96 wells
EUR 685
Description: A competitive ELISA for quantitative measurement of Rat Helicobacter pylori IgG in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Helicobacter pylori sIgA ELISA kit

E02H0269-192T 192 tests
EUR 1270
Description: A competitive ELISA for quantitative measurement of Rat Helicobacter pylori sIgA in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Helicobacter pylori sIgA ELISA kit

E02H0269-48 1 plate of 48 wells
EUR 520
Description: A competitive ELISA for quantitative measurement of Rat Helicobacter pylori sIgA in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Helicobacter pylori sIgA ELISA kit

E02H0269-96 1 plate of 96 wells
EUR 685
Description: A competitive ELISA for quantitative measurement of Rat Helicobacter pylori sIgA in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Helicobacter pylori IgM ELISA kit

E02H0271-192T 192 tests
EUR 1270
Description: A competitive ELISA for quantitative measurement of Rat Helicobacter pylori IgM in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Helicobacter pylori IgM ELISA kit

E02H0271-48 1 plate of 48 wells
EUR 520
Description: A competitive ELISA for quantitative measurement of Rat Helicobacter pylori IgM in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Helicobacter pylori IgM ELISA kit

E02H0271-96 1 plate of 96 wells
EUR 685
Description: A competitive ELISA for quantitative measurement of Rat Helicobacter pylori IgM in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog Helicobacter pylori IgA ELISA kit

E08H0261-192T 192 tests
EUR 1270
Description: A competitive ELISA for quantitative measurement of Canine Helicobacter pylori IgA in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog Helicobacter pylori IgA ELISA kit

E08H0261-48 1 plate of 48 wells
EUR 520
Description: A competitive ELISA for quantitative measurement of Canine Helicobacter pylori IgA in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog Helicobacter pylori IgA ELISA kit

E08H0261-96 1 plate of 96 wells
EUR 685
Description: A competitive ELISA for quantitative measurement of Canine Helicobacter pylori IgA in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog Helicobacter pylori CagA ELISA kit

E08H0262-192T 192 tests
EUR 1270
Description: A competitive ELISA for quantitative measurement of Canine Helicobacter pylori CagA in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog Helicobacter pylori CagA ELISA kit

E08H0262-48 1 plate of 48 wells
EUR 520
Description: A competitive ELISA for quantitative measurement of Canine Helicobacter pylori CagA in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog Helicobacter pylori CagA ELISA kit

E08H0262-96 1 plate of 96 wells
EUR 685
Description: A competitive ELISA for quantitative measurement of Canine Helicobacter pylori CagA in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog Helicobacter pylori antibody ELISA kit

E08H0263-192T 192 tests
EUR 1270
Description: A competitive ELISA for quantitative measurement of Canine Helicobacter pylori antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog Helicobacter pylori antibody ELISA kit

E08H0263-48 1 plate of 48 wells
EUR 520
Description: A competitive ELISA for quantitative measurement of Canine Helicobacter pylori antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog Helicobacter pylori antibody ELISA kit

E08H0263-96 1 plate of 96 wells
EUR 685
Description: A competitive ELISA for quantitative measurement of Canine Helicobacter pylori antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog Helicobacter pylori Hsp60 ELISA kit

E08H0267-192T 192 tests
EUR 1270
Description: A sandwich ELISA for quantitative measurement of Canine Helicobacter pylori Hsp60 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog Helicobacter pylori Hsp60 ELISA kit

E08H0267-48 1 plate of 48 wells
EUR 520
Description: A sandwich ELISA for quantitative measurement of Canine Helicobacter pylori Hsp60 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog Helicobacter pylori Hsp60 ELISA kit

E08H0267-96 1 plate of 96 wells
EUR 685
Description: A sandwich ELISA for quantitative measurement of Canine Helicobacter pylori Hsp60 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog Helicobacter pylori IgG ELISA kit

E08H0268-192T 192 tests
EUR 1270
Description: A competitive ELISA for quantitative measurement of Canine Helicobacter pylori IgG in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog Helicobacter pylori IgG ELISA kit

E08H0268-48 1 plate of 48 wells
EUR 520
Description: A competitive ELISA for quantitative measurement of Canine Helicobacter pylori IgG in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog Helicobacter pylori IgG ELISA kit

E08H0268-96 1 plate of 96 wells
EUR 685
Description: A competitive ELISA for quantitative measurement of Canine Helicobacter pylori IgG in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Quantification of Ammonia Oxidizing Bacterial Abundances in Environmental Samples by Quantitative-PCR

Quantitative-PCR (qPCR) enables the quantification of specific DNA targets, such as functional or phylogenetic marker genes associated with environmental samples. During each qPCR cycle, the number of copies of a gene (or region) of interest in DNA samples is determined in real time using a fluorescence-based label and compared to a standard serial dilution.
Here, we describe a qPCR method to quantify the ammonia oxidizing bacteria involved in the first step of nitrification, using the amoA gene as a proxy of their abundance. The preparation of the standards from environmental samples and qPCR is presented in detail for specifically quantifying microbial abundance in environmental samples such as soil.

Peptide Nucleic Acid (PNA) Clamps to Reduce Co-amplification of Plant DNA During PCR Amplification of 16S rRNA Genes from Endophytic Bacteria

High-throughput sequencing of universal bacterial 16S rRNA gene (16S rDNA) amplicons is a routine method for characterizing bacterial diversity in a range of environments. For eukaryotic host-associated communities, however, plastid and mitochondrial genes are often co-amplified with, and greatly outnumber, bacterial 16S rDNA.
This makes it difficult to obtain sufficient numbers of target 16S rDNA sequences to characterize the diversity of endophytic bacterial communities. This chapter describes a method that improves the amplification of bacterial 16S rDNA from plant tissues by using a peptide nucleic acid (PNA) PCR clamp. The PNA clamp selectively binds to a targeted region of the plant genome and inhibits its amplification during PCR. PNA clamps are especially useful for characterizing bacterial communities on plant tissues with lower levels of microbial colonization such as the root tips and leaves.

Retrospective Post-mortem SARS-CoV-2 RT-PCR of Autopsies with COVID-19-Suggestive Pathology Supports the Absence of Lethal Community Spread in Basel, Switzerland, before February 2020

Introduction: Coronavirus disease 2019 (COVID-19) caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has rapidly spread around the world. While the first case was recorded in Hubei in December 2019, the extent of early community spread in Central Europe before this period is unknown. A high proportion of asymptomatic cases and undocumented infections, high transmissibility, and phylogenetic genomic diversity have engendered the controversial possibility of early international community spread of SARS-CoV-2 before its emergence in China.
Methods: To assess the early presence of lethal COVID-19 in Switzerland, we retrospectively performed an analysis of deaths at University Hospital Basel between October 2019 and February 2020 (n = 310), comparing the incidence of clinical causes of death with March 2020 (n = 72), the month during which the first lethal COVID-19 cases in Basel were reported. Trends of COVID-19-suggestive sequelae, such as bronchopneumonia with organization, acute respiratory distress syndrome (ARDS), or pulmonary embolisms (PE) were evaluated.
In cases where autopsy was performed (n = 71), analogous analyses were conducted on the cause of death and pulmonary histological findings. Eight cases with a COVID-19-suggestive clinical history and histopathology between October 2019 and February 2020, and 3 cases before October 2019, were selected for SARS-CoV-2 RT-PCR.

HCV Seroconversion Panel Donor# 68896 (6 X 1 mL)

HCV10009 6 X 1 mL
EUR 986
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HCV Seroconversion Panel Donor# 65470 (7 X 1 mL)

HCV10010 7 X 1 mL
EUR 986
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HCV Seroconversion Panel Donor# 66666 (13 X 1 mL)

HCV10011 13 X 1 mL
EUR 986
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HCV Seroconversion Panel Donor# 66747 (13 X 1 mL)

HCV10012 13 X 1 mL
EUR 986
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HCV Seroconversion Panel Donor# 67356 (10 X 1 mL)

HCV10013 10 X 1 mL
EUR 986
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HCV Seroconversion Panel Donor# 68157 (4 X 1 mL)

HCV10014 4 X 1 mL
EUR 986
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HCV Seroconversion Panel Donor# 68628 (12 X 1 mL)

HCV10016 12 X 1 mL
EUR 986
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HCV Seroconversion Panel Donor# 65064 (16 X 1 mL)

HCV10017 16 X 1 mL
EUR 1548.64
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HCV Seroconversion Panel Donor# 69142 (8 X 1 mL)

HCV10019 8 X 1 mL
EUR 986
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HCV Seroconversion Panel Donor# 68744 (13 X 1 mL)

HCV10020 13 X 1 mL
EUR 986
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HCV Seroconversion Panel Donor# 68470 (14 X 1 mL)

HCV10021 14 X 1 mL
EUR 986
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HCV Seroconversion Panel Donor# 68450 (11 X 1 mL)

HCV10022 11 X 1 mL
EUR 986
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HCV Seroconversion Panel Donor# 67505 (21 X 1 mL)

HCV10023 21 X 1 mL
EUR 986
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HCV Seroconversion Panel Donor# 66719 (8 X 1 mL)

HCV10024 8 X 1 mL
EUR 986
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HCV Seroconversion Panel Donor# 66801 (11 X 1 mL)

HCV10025 11 X 1 mL
EUR 986
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HCV Seroconversion Panel Donor# 67391 (12 X 1 mL)

HCV10026 12 X 1 mL
EUR 1548.64
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HCV Seroconversion Panel Donor# 66751 (8 X 1 mL)

HCV10027 8 X 1 mL
EUR 986
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HCV Seroconversion Panel Donor# 67116 (7 X 1 mL)

HCV10028 7 X 1 mL
EUR 986
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HCV Seroconversion Panel Donor# 67539 (16 X 1 mL)

HCV10029 16 X 1 mL
EUR 986
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HCV Seroconversion Panel Donor# 65977 (8 X 1 mL)

HCV10030 8 X 1 mL
EUR 986
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HCV Seroconversion Panel Donor# 66113 (16 X 1 mL)

HCV10032 16 X 1 mL
EUR 986
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HCV Seroconversion Panel Donor# 68629 (10 X 1 mL)

HCV10033 10 X 1 mL
EUR 986
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HCV Seroconversion Panel Donor# 68223 (13 X 1 mL)

HCV10036 13 X 1 mL
EUR 986
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HCV Seroconversion Panel Donor# 65643 (10 X 1 mL)

HCV10043 10 X 1 mL
EUR 986
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HCV Seroconversion Panel Donor# 65982 (4 X 1 mL)

HCV10044 4 X 1 mL
EUR 986
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HCV Seroconversion Panel Donor# 66074 (5 X 1 mL)

HCV10045 5 X 1 mL
EUR 986
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HCV Seroconversion Panel Donor# 67766 (15 X 1 mL)

HCV10050 15 X 1 mL
EUR 986
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HCV Seroconversion Panel Donor# 67787 (14 X 1 mL)

HCV10051 14 X 1 mL
EUR 986
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HCV Seroconversion Panel Donor# 67861 (12 X 1 mL)

HCV10052 12 X 1 mL
EUR 986
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HCV Seroconversion Panel Donor# 420245049 (7 X 1 mL)

HCV10057 7 X 1 mL
EUR 1548.64
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HCV Seroconversion Panel Donor# 420299823 (7 X 1 mL)

HCV10058 7 X 1 mL
EUR 1548.64
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HCV Seroconversion Panel Donor# 490104584 (8 X 1 mL)

HCV10062 8 X 1 mL
EUR 1548.64
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HCV Seroconversion Panel Donor# 90163052 (7 X 1 mL)

HCV10071 7 X 1 mL
EUR 1548.64
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HCV Seroconversion Panel Donor# 72237 (9 X 1 mL)

HCV10165 9 X 1 mL
EUR 1548.64
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HCV Seroconversion Panel Donor# 70225 (5 X 1 mL)

HCV10185 5 X 1 mL
EUR 1548.64
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HCV Seroconversion Panel Donor# 61025 (5 X 1 mL)

HCV6217 5 X 1 mL
EUR 928.8
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HCV Seroconversion Panel Donor# 61251 (8 X 1 mL)

HCV6218 8 X 1 mL
EUR 928.8
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HCV Seroconversion Panel Donor# 60749 (14 X 1 mL)

HCV6219 14 X 1 mL
EUR 928.8
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HCV Seroconversion Panel Donor# 63792 (6 X 1 mL)

HCV6220 6 X 1 mL
EUR 928.8
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HCV Seroconversion Panel Donor# 62258 (8 X 1 mL)

HCV6221 8 X 1 mL
EUR 928.8
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HCV Seroconversion Panel Donor# 62286 (8 X 1 mL)

HCV6222 8 X 1 mL
EUR 1548.64
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HCV Seroconversion Panel Donor# 62507 (22 X 1 mL)

HCV6223 22 X 1 mL
EUR 928.8
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HCV Seroconversion Panel Donor# 62680 (6 X 1 mL)

HCV6224 6 X 1 mL
EUR 1548.64
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HCV Seroconversion Panel Donor# 62999 (19 X 1 mL)

HCV6225 19 X 1 mL
EUR 1548.64
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HCV Seroconversion Panel Donor# 63135 (12 X 1 mL)

HCV6226 12 X 1 mL
EUR 1548.64
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HCV Seroconversion Panel Donor# 63318 (7 X 1 mL)

HCV6227 7 X 1 mL
EUR 1548.64
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HCV Seroconversion Panel Donor# 62886 (12 X 1 mL)

HCV6228 12 X 1 mL
EUR 1548.64
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HCV Seroconversion Panel Donor# 62804 (8 X 1 mL)

HCV6229 8 X 1 mL
EUR 1548.64
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HCV Seroconversion Panel Donor# 63625 (8 X 1 mL)

HCV9041 8 X 1 mL
EUR 1548.64
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HCV Seroconversion Panel Donor# 63792 (6 X 1 mL)

HCV9042 6 X 1 mL
EUR 928.8
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HCV Seroconversion Panel Donor# 63869 (6 X 1 mL)

HCV9043 6 X 1 mL
EUR 928.8
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HCV Seroconversion Panel Donor# 63911 (6 X 1 mL)

HCV9044 6 X 1 mL
EUR 1548.64
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HCV Seroconversion Panel Donor# 64150 (8 X 1 mL)

HCV9045 8 X 1 mL
EUR 1548.64
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HCV Seroconversion Panel Donor# 64254 (5 X 1 mL)

HCV9046 5 X 1 mL
EUR 1548.64
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HCV Seroconversion Panel Donor# 64273 (10 X 1 mL)

HCV9047 10 X 1 mL
EUR 1548.64
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HCV Seroconversion Panel Donor# 64437 (8 X 1 mL)

HCV9048 8 X 1 mL
EUR 928.8
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HCV Seroconversion Panel Donor# 64766 (5 X 1 mL)

HCV9049 5 X 1 mL
EUR 928.8
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HCV Seroconversion Panel Donor# 66158 (13 X 1 mL)

HCV9050 13 X 1 mL
EUR 1548.64
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HCV Seroconversion Panel Donor# 66626 (8 X 1 mL)

HCV9054 8 X 1 mL
EUR 1548.64
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HCV Seroconversion Panel Donor# 66732 (11 X 1 mL)

HCV9055 11 X 1 mL
EUR 1548.64
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HCV Seroconversion Panel Donor# 66861 (11 X 1 mL)

HCV9057 11 X 1 mL
EUR 1548.64
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HCV Seroconversion Panel Donor# 66647 (5 X 1 mL)

HCV9058 5 X 1 mL
EUR 1548.64
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HCV Seroconversion Panel Donor# 22708D (5 X 1 mL)

HCV9094 5 X 1 mL
EUR 1548.64
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HCV Seroconversion Panel Donor# 1038851 (5 X 1 mL)

HCV9095 5 X 1 mL
EUR 1548.64
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HCV Pre/Post Seroconversion Panel Donor# 66822 (5 X 1 mL)

HCV10235 5 X 1 mL
EUR 512.8
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HIV Seroconversion Panel Donor# 65404 (7 X mL)

HIV9013 7 X mL
EUR 1548.64
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EBV Seroconversion Panel Donor# 283843421 (14 X 1 mL)

EBV9097 14 X 1 mL
EUR 1548.64
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