Tag: miniprep plasmid isolation
Identification of proteins associated with splicing factors Ntr1, Ntr2, Brr2 and Gpl1 in the fission yeast Schizosaccharomyces pombe.
The spliceosome is a posh molecular machine assembled from many parts, which catalyzes the elimination of introns from mRNA precursors. Our earlier examine revealed that the Nrl1 (NRDE-2 like 1) protein associates with spliceosome proteins and regulates pre-mRNA splicing and homologous recombination-dependent R-loop formation within the fission yeast Schizosaccharomyces pombe. Right here, we determine proteins related…Read More
Epitope-dependent thermodynamic signature of single-domain antibodies against hen egg lysozyme
A considerable physique of labor has been carried out describing the structural options of the advanced between single-domain antibodies (VHHs) and antigens, and the preeminence for epitopes positioned at concave surfaces of the antigen. Nonetheless, the thermodynamic foundation of binding is much much less clear. Right here, we have now analyzed the energetic profiles of…Read More