Evaluating the performance of targeted sequence capture, RNA-Seq, and degenerate-primer PCR cloning for sequencing the largest mammalian multigene family.

Evaluating the performance of targeted sequence capture, RNA-Seq, and degenerate-primer PCR cloning for sequencing the largest mammalian multigene family.

Multigene households evolve from single-copy ancestral genes by way of duplication, and usually encode proteins essential to key organic processes. Molecular analyses of those gene households require high-confidence sequences, however the excessive sequence similarity of the members can create challenges for sequencing and downstream analyses. Specializing in the frequent vampire bat, Desmodus rotundus, we evaluated…

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