Tag: pcr steps
Identification of the ‘Haryejosaeng’ mandarin cultivar by multiplex PCR-based SNP genotyping
Development of a PCR-RFLP method for detection of D614G mutation in SARS-CoV-2 In late 2019, an outbreak of respiratory disease named COVID-19 started in the world. To date, thousands of cases of infection are reported worldwide. Most researchers focused on epidemiology and clinical features of COVID-19, and a small part of studies was performed to evaluate…
Read MoreA Novel Multiplex RT-PCR Assay for Simultaneous Detection of Dengue and Chikungunya Viruses
GLAD-PCR Assay of R(5mC)GY Sites in the Regulatory Region of Tumor-Suppressor Genes Associated with Gastric Cancer At early stages of carcinogenesis, the regulatory regions of some tumor suppressor genes become aberrantly methylated at RCGY sites, which are substrates of DNA methyltransferase Dnmt3. Identification of aberrantly methylated sites in tumor DNA is considered to be the…
Read MoreBDNF Val66Met Polymorphism, the Allele-Specific Analysis by QRT-PCR – a Novel Protocol
Molecular Blood Group Screening in Donors from Arabian Countries and Iran Using High-Throughput MALDI-TOF Mass Spectrometry and PCR-SSP Background and aims: Only little is known about blood groups other than ABO blood groups and Rhesus factors in Arabian countries and Iran. During the last years, increased migration to Central Europe has put a focus on the question…
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