Tag: shrna plasmid construction
Agrobacterium tumefaciens-mediated transformation of Pinus pinea L. cotyledons: an assessment of factors influencing the efficiency of uidA gene transfer.
This examine is the primary report of a protocol for switch and expression of overseas chimeric genes into cotyledons excised from Pinus pinea L. embryos. Agrobacterium tumefaciens EHA105 harbouring the plasmid p35SGUSint was extra infective than LBA4404 or C58 GV3850, as decided by the share of cotyledons displaying uidA expression. Elements which considerably affected the…Read More
The use of the replication region of plasmid pRS7 from Oenococcus oeni as a putative tool to generate cloning vectors for lactic acid bacteria.
A chimeric plasmid, pRS7Rep (6.1 kb), was constructed utilizing the replication area of pRS7, a big plasmid from Oenococcus oeni, and pEM64, a plasmid derived from pIJ2925 and containing a gene for resistance to chloramphenicol. pRS7Rep is a shuttle vector that replicates in Escherichia coli utilizing its pIJ2925 part and in lactic acid micro organism…Read More