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RNA complementary to a herpesvirus alpha gene mRNA is prominent in latently infected neurons.
In preliminary makes an attempt to outline the molecular occasions chargeable for the latent state of herpes simplex virus, in situ hybridization was utilized to seek for virally encoded RNA transcripts in latently contaminated sensory neurons. The usage of cloned probes representing the whole viral genome indicated that transcripts encoded inside terminal repeats have been current. When…Read More
Combinatorial Fine-Tuning of GNA1 and GlmS Expression by 5′-Terminus Fusion Engineering Leads to Overproduction of N-Acetylglucosamine in Bacillus subtilis.
Glucosamine-6-phosphate N-acetyltransferase (GNA1) that catalyzes acetyl switch from acetyl-coenzyme A to glucosamine-6-phosphate (GlcN-6P), and glutamine-fructose-6-phosphate aminotransferase (GlmS) that catalyzes the formation of GlcN-6P from fructose-6-phosphate (Fru-6P), are two key enzymes in Bacillus subtilis for the bioproduction of N-acetylglucosamine (GlcNAc), a nutraceutical that has varied functions in healthcare. On this examine, the expression of GNA1 and…Read More