A p53/CPEB2 negative feedback loop regulates renal cancer cell proliferation and migration

A p53/CPEB2 negative feedback loop regulates renal cancer cell proliferation and migration

The tumor suppressor p53 transactivates the expression of a number of genes to exert its multifaceted features and in the end maintains genome stability. Thus, most cancers cells develop numerous mechanisms to decrease p53 expression and bypass the cell cycle checkpoint.
On this examine, we recognized the gene encoding RNA-binding protein cytoplasmic polyadenylation element-binding protein 2 (CPEB2) as a p53 goal. In flip, CPEB2 decreases p53 messenger RNA stability and translation to fine-tune p53 expression. Particularly, we confirmed that CPEB2 binds the cytoplasmic polyadenylation components within the p53 3′-untranslated area, and the RNA recognition motif and zinc finger (ZF) domains of CPEB2 are required for this binding.
Moreover, we discovered that CPEB2 was upregulated in renal most cancers tissues and promotes the renal most cancers cell proliferation and migration. The oncogenic impact of CPEB2 is partially depending on unfavorable suggestions regulation of p53. General, we establish a novel regulatory suggestions loop between p53 and CPEB2 and show that CPEB2 promotes tumor development by inactivating p53, suggesting that CPEB2 is a possible therapeutic goal in human renal most cancers.

The RNA Binding Protein CPEB2 Regulates Hormone Sensing in Mammary Gland Improvement and Luminal Breast Most cancers

Organogenesis is directed by coordinated cell proliferation and differentiation packages. The hierarchical networks of transcription components driving mammary gland growth and performance have been broadly studied. Nevertheless, the contribution of posttranscriptional gene expression reprogramming stays largely unexplored.
The three’ untranslated areas of messenger RNAs (mRNAs) include combinatorial ensembles of cis-regulatory components that outline transcript-specific regulation of protein synthesis by means of their cognate RNA binding proteins. We analyze the contribution of the RNA binding cytoplasmic polyadenylation element-binding (CPEB) protein household, which collectively regulate mRNA translation for about 30% of the genome.
We discover that CPEB2 is required for the combination of hormonal signaling by controlling the protein expression from a subset of ER/PR- regulated transcripts. Moreover, CPEB2 is vital for the event of ER-positive breast tumors. This work uncovers a beforehand unknown gene expression regulation stage in breast morphogenesis and tumorigenesis, coordinating sequential transcriptional and posttranscriptional layers of gene expression regulation.

Corrigendum to: Cytoplasmic polyadenylation component binding protein 2 (CPEB2) is required for tight-junction meeting for institution of porcine trophectoderm epithelium.

Cytoplasmic polyadenylation component binding protein (CPEB) is an RNA-binding protein that promotes elongation of poly(A) tails and regulates mRNA translation. CPEB depletion in mammary epithelium is understood to disrupt tight-junction (TJ) meeting by way of mislocalisation of tight junction protein 1 (TJP1), however the function of CPEB within the organic features related to TJs has not but been studied.
The target of this examine was to analyze the roles of CPEB2 throughout porcine parthenote growth. CPEB2 was detected in each the nuclei and apical cytoplasm on the 4- and 8-cell phases and was localised to cell-cell contact after the initiation of the morula stage. Its depletion led to retarded blastocyst formation attributable to impaired TJ meeting.
Furthermore, transcription of TJ-associated genes, together with TJP1, Coxsackie virus and adenovirus receptor (CXADR) and occludin (OCLN), was not affected, however the corresponding proteins weren’t correctly localised on the apical cell membrane in morulae, suggesting that CPEB2 confers mRNA stability or determines subcellular localisation for translation.
Remarkably lowered relative ranges of TJP1 transcripts bearing the three’-untranslated area have been famous, indicating that CPEB2 mediates TJP1 mRNA stability. In conclusion, our findings show that due to its regulation of TJP1, CPEB2 is required for TJ meeting throughout porcine blastocyst growth.

Splice variants of cytosolic polyadenylation element-binding protein 2 (CPEB2) differentially regulate pathways linked to most cancers metastasis.

The translational regulator cytosolic polyadenylation element-binding protein 2 (CPEB2) has two isoforms, CPEB2A and CPEB2B, derived by various splicing of RNA right into a mature kind that both contains or excludes exon 4. Beforehand, we reported that this splicing occasion is extremely dysregulated in aggressive types of breast cancers, which overexpress CPEB2B.
The lack of CPEB2A with a concomitant enhance in CPEB2B was additionally required for breast most cancers cells to withstand cell demise due to detachment (anoikis resistance) and metastasize in vivo To look at the mechanism by which CPEB2 isoforms mediate opposing results on cancer-related phenotypes, we used subsequent technology sequencing of triple unfavorable breast most cancers cells by which the isoforms have been particularly down-regulated.
Down-regulation of the CPEB2B isoform inhibited pathways driving the epithelial-to-mesenchymal transition and hypoxic response, whereas down-regulation of the CPEB2A isoform didn’t have this impact. Inspecting key nodes of those pathways confirmed that CPEB2B induced the expression of regulatory DNA trans-factors (e.g. HIF1α and TWIST1).
Particularly, CPEB2B functioned as a translational activator of TWIST1 and HIF1α. Purposeful research confirmed that particular down-regulation of both HIF1α or TWIST1 inhibited the flexibility of CPEB2B to induce the acquisition of anoikis resistance and drive metastasis. General, this examine demonstrates that CPEB2 various splicing is a significant regulator of key mobile pathways linked to anoikis resistance and metastasis.
 A p53/CPEB2 negative feedback loop regulates renal cancer cell proliferation and migration

TUG1 mediates methotrexate resistance in colorectal most cancers by way of miR-186/CPEB2 axis.

Colorectal most cancers (CRC) is a standard malignancy, most of which stay unresponsive to chemotherapy. Methotrexate (MTX) is likely one of the earliest cytotoxic medication and serves as an anti-metabolite and anti-folate chemotherapy for numerous varieties of most cancers. Nevertheless, MTX resistance prevents its medical software in most cancers remedy.
Thereby, overcoming the drug resistance is an alternate technique to maximise the efficacy of MTX therapies in clinics. Lengthy non-coding RNAs (lncRNAs) have gained widespread consideration lately. Increasingly more evidences have proven that lncRNAs play regulatory roles in numerous organic actions and illness development together with drug resistance in most cancers cells.
Right here, we noticed lncRNA TUG1 was related to the MTX resistant in colorectal most cancers cells. Firstly, quantitative evaluation indicated that TUG1 was considerably elevated in tumors which have been proof against MTX remedy. TUG1 knockdown re-sensitized the MTX resistance in colorectal most cancers cells, which have been MTX-resistant colorectal cell line.

CPEB2 siRNA

20-abx912652
  • EUR 551.00
  • EUR 732.00
  • 15 nmol
  • 30 nmol
  • Shipped within 5-10 working days.

CPEB2 Blocking Peptide

33R-2191 100 ug
EUR 180
Description: A synthetic peptide for use as a blocking control in assays to test for specificity of CPEB2 antibody, catalog no. 70R-1357

CPEB2 Blocking Peptide

33R-2978 100 ug
EUR 180
Description: A synthetic peptide for use as a blocking control in assays to test for specificity of CPEB2 antibody, catalog no. 70R-4851

CPEB2 Blocking Peptide

33R-8661 100 ug
EUR 180
Description: A synthetic peptide for use as a blocking control in assays to test for specificity of CPEB2 antibody, catalog no. 70R-4879

CPEB2 Antibody, HRP conjugated

1-CSB-PA767205LB01HU
  • EUR 317.00
  • EUR 335.00
  • 100ug
  • 50ug
  • Form: Liquid
  • Buffer: Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 >95%, Protein G purified
Description: A polyclonal antibody against CPEB2. Recognizes CPEB2 from Human. This antibody is HRP conjugated. Tested in the following application: ELISA

CPEB2 Antibody, FITC conjugated

1-CSB-PA767205LC01HU
  • EUR 317.00
  • EUR 335.00
  • 100ug
  • 50ug
  • Form: Liquid
  • Buffer: Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 >95%, Protein G purified
Description: A polyclonal antibody against CPEB2. Recognizes CPEB2 from Human. This antibody is FITC conjugated. Tested in the following application: ELISA

CPEB2 Antibody, Biotin conjugated

1-CSB-PA767205LD01HU
  • EUR 317.00
  • EUR 335.00
  • 100ug
  • 50ug
  • Form: Liquid
  • Buffer: Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 >95%, Protein G purified
Description: A polyclonal antibody against CPEB2. Recognizes CPEB2 from Human. This antibody is Biotin conjugated. Tested in the following application: ELISA

Mouse Cpeb2 ELISA KIT

ELI-26419m 96 Tests
EUR 865

Human CPEB2 shRNA Plasmid

20-abx965101
  • EUR 801.00
  • EUR 1121.00
  • 150 µg
  • 300 µg
  • Shipped within 15-20 working days.

Human CPEB2 ELISA KIT

ELI-33931h 96 Tests
EUR 824

CPEB2 ORF Vector (Human) (pORF)

ORF017672 1.0 ug DNA
EUR 405

Cpeb2 ORF Vector (Mouse) (pORF)

ORF041911 1.0 ug DNA
EUR 506

Cpeb2 ORF Vector (Mouse) (pORF)

ORF041912 1.0 ug DNA
EUR 506

CPEB2 ELISA Kit (Mouse) (OKCA01653)

OKCA01653 96 Wells
EUR 846
Description: Description of target: May play a role in translational regulation of stored mRNAs in transcriptionally inactive haploid spermatids. Binds to poly(U) RNA oligomers. ;Species reactivity: Mouse;Application: ;Assay info: Assay Methodology: Quantitative Sanadwich ELISA;Sensitivity: 7 pg/mL

CPEB2 sgRNA CRISPR Lentivector set (Human)

K0499201 3 x 1.0 ug
EUR 339

Cpeb2 sgRNA CRISPR Lentivector set (Mouse)

K3133201 3 x 1.0 ug
EUR 339

CPEB2 sgRNA CRISPR Lentivector (Human) (Target 1)

K0499202 1.0 ug DNA
EUR 154

CPEB2 sgRNA CRISPR Lentivector (Human) (Target 2)

K0499203 1.0 ug DNA
EUR 154

CPEB2 sgRNA CRISPR Lentivector (Human) (Target 3)

K0499204 1.0 ug DNA
EUR 154

Cpeb2 sgRNA CRISPR Lentivector (Mouse) (Target 1)

K3133202 1.0 ug DNA
EUR 154

Cpeb2 sgRNA CRISPR Lentivector (Mouse) (Target 2)

K3133203 1.0 ug DNA
EUR 154

Cpeb2 sgRNA CRISPR Lentivector (Mouse) (Target 3)

K3133204 1.0 ug DNA
EUR 154

CPEB2 Protein Vector (Mouse) (pPB-C-His)

PV167642 500 ng
EUR 1065

CPEB2 Protein Vector (Mouse) (pPB-N-His)

PV167643 500 ng
EUR 1065

CPEB2 Protein Vector (Mouse) (pPM-C-HA)

PV167644 500 ng
EUR 1065

CPEB2 Protein Vector (Mouse) (pPM-C-His)

PV167645 500 ng
EUR 1065

CPEB2 Protein Vector (Mouse) (pPB-C-His)

PV167646 500 ng
EUR 1065

CPEB2 Protein Vector (Mouse) (pPB-N-His)

PV167647 500 ng
EUR 1065

CPEB2 Protein Vector (Mouse) (pPM-C-HA)

PV167648 500 ng
EUR 1065

CPEB2 Protein Vector (Mouse) (pPM-C-His)

PV167649 500 ng
EUR 1065

CPEB2 Protein Vector (Human) (pPB-C-His)

PV070685 500 ng
EUR 552

CPEB2 Protein Vector (Human) (pPB-N-His)

PV070686 500 ng
EUR 552

CPEB2 Protein Vector (Human) (pPM-C-HA)

PV070687 500 ng
EUR 552

CPEB2 Protein Vector (Human) (pPM-C-His)

PV070688 500 ng
EUR 552

Cpeb2 3'UTR GFP Stable Cell Line

TU154292 1.0 ml Ask for price

Cpeb2 3'UTR Luciferase Stable Cell Line

TU104292 1.0 ml Ask for price

CPEB2 3'UTR GFP Stable Cell Line

TU054935 1.0 ml
EUR 4617

CPEB2 3'UTR Luciferase Stable Cell Line

TU004935 1.0 ml
EUR 4617

Cytoplasmic Polyadenylation Element Binding Protein 2 (CPEB2) Antibody

20-abx318745
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug
  • Shipped within 5-10 working days.

Cytoplasmic Polyadenylation Element Binding Protein 2 (CPEB2) Antibody (HRP)

20-abx316337
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug
  • Shipped within 5-10 working days.

Cytoplasmic Polyadenylation Element Binding Protein 2 (CPEB2) Antibody (FITC)

20-abx316338
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug
  • Shipped within 5-10 working days.

Cytoplasmic Polyadenylation Element Binding Protein 2 (CPEB2) Antibody (Biotin)

20-abx316339
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug
  • Shipped within 5-10 working days.
Moreover, bioinformatics evaluation confirmed that miR-186 may immediately bind to TUG1, suggesting TUG1 would possibly labored as a ceRNA to sponge miR-186. Extensively, our examine additionally confirmed that CPEB2 was the direct goal of miR-186 in colorectal most cancers cells. Taken collectively, our examine means that lncRNA TUG1 mediates MTX resistance in colorectal most cancers by way of miR-186/CPEB2 axis.

Leave a Reply

Your email address will not be published. Required fields are marked *