CD34 + stem cell counting using labeled immobilized anti-CD34 antibody onto magnetic nanoparticles and EasyCounter BC image cytometer

CD34 + stem cell counting using labeled immobilized anti-CD34 antibody onto magnetic nanoparticles and EasyCounter BC image cytometer

The power of immobilized conjugate anti-CD34+ monoclonal antibody-dR110 and free conjugate anti-CD45+ monoclonal antibody-ATTO620 to exactly enumerate CD34+ stem cells and CD45+ cells in apheresis samples had been evaluated. The conjugates anti-CD34+ antibody-dR110 and anti-CD45+– antibody-ATTO620 had been ready.
Functionalized magnetic nanoparticles (MNPs) had been synthesized. The anti-CD34+ antibody-dR110 conjugate was immobilized on the modified MNPs utilizing a carbodiimide methodology. The stem cell depend in thawed apheresis samples was decided utilizing the free and the immobilized conjugate anti-CD34+ antibody-dR110 on MNPs and a picture cell counter EasyCounter BC.
A better stem cell depend and extra correct outcomes had been obtained with the immobilized conjugate, as a result of a separation and focus of the stem cells certain to antibody-dR110 on MNPs by exterior magnet had been carried out. Coefficients of variation of CD34+ cell depend in apheresis samples, decided by EasyCounter BC, had been ranged from 5.5 to six.9% and people of CD45+ cell depend from 3.
Eight to 4.7%. The viability of CD34+ cells was excessive from 98.5 to 99.6%. It was discovered that correlation coefficient between the stream cytometer and automated cell counter, utilizing free anti-CD34+ antibody-dR110 was 0.94, and when utilizing immobilized anti-CD34+antibody-dR110 on MNPs, the correlation coefficient was 0.97.

Floor Coating of Polytetrafluoroethylene with Extracellular Matrix and Anti-CD34 Antibodies Facilitates Endothelialization and Inhibits Platelet Adhesion Below Sheer Stress.

Expanded polytetrafluoroethylene (ePTFE) polymers don’t help endothelialization due to nonconductive traits in direction of mobile attachment. Interior floor modification of the grafts can enhance endothelialization and enhance the long-term patency charge of the ePTFE vascular grafts.
Right here we reported a way of inner-surface modification of ePTFE vascular graft with extracellular matrix (ECM) and CD34 monoclonal antibodies (CD34 mAb) to stimulate the adhesion and proliferation of circulating endothelial progenitor cells on ePTFE graft to boost graft endothelialization.
The interior floor of ECM-coated ePTFE grafts had been linked with CD34 mAb within the presence of 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide/N-hydroxysuccinimide (EDC/NHS) resolution and the physicochemical properties, floor morphology, biocompatibility, and hemocompatibility of the grafts had been studied.
The hydrophilicity of CD34 mAb-coated graft interior floor was considerably improved. Fourier remodel infrared spectroscopy evaluation confirmed ECM and CD34 mAb cross-linking within the ePTFE vascular grafts with our methodology. Scanning electron microscopy evaluation confirmed protein layer masking uniformly on the interior floor of the modified grafts.
The cell-counting kit-8 (CCK-8) assay confirmed that the modified graft has no apparent cytotoxicity. The modified graft confirmed a low hemolytic charge (0.9%) within the direct contact hemolysis check, suggesting the modification improved hemocompatibility of biopolymers. The modification additionally decreased adhesion of platelets, whereas considerably elevated the adhesion of endothelial cells on the grafts.
We conclude that our methodology permits ePTFE polymers modification with ECM and CD34 mAb, facilitates endothelialization, and inhibits platelet adhesion on the grafts, thus could enhance the long-term patency charge of the prosthetic bypass grafts.

Human Cluster Of Differentiation 34 (CD34) ELISA Kit

DLR-CD34-Hu-48T 48T
EUR 498
Description: A sandwich quantitative ELISA assay kit for detection of Human Cluster Of Differentiation 34 (CD34) in samples from tissue homogenates, cell lysates or other biological fluids.

Human Cluster Of Differentiation 34 (CD34) ELISA Kit

DLR-CD34-Hu-96T 96T
EUR 647
Description: A sandwich quantitative ELISA assay kit for detection of Human Cluster Of Differentiation 34 (CD34) in samples from tissue homogenates, cell lysates or other biological fluids.

Mouse Cluster Of Differentiation 34 (CD34) ELISA Kit

DLR-CD34-Mu-48T 48T
EUR 508
Description: A sandwich quantitative ELISA assay kit for detection of Mouse Cluster Of Differentiation 34 (CD34) in samples from tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Mouse Cluster Of Differentiation 34 (CD34) ELISA Kit

DLR-CD34-Mu-96T 96T
EUR 661
Description: A sandwich quantitative ELISA assay kit for detection of Mouse Cluster Of Differentiation 34 (CD34) in samples from tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Human Cluster Of Differentiation 34 (CD34) ELISA Kit

RDR-CD34-Hu-48Tests 48 Tests
EUR 522

Human Cluster Of Differentiation 34 (CD34) ELISA Kit

RDR-CD34-Hu-96Tests 96 Tests
EUR 724

Mouse Cluster Of Differentiation 34 (CD34) ELISA Kit

RDR-CD34-Mu-48Tests 48 Tests
EUR 534

Mouse Cluster Of Differentiation 34 (CD34) ELISA Kit

RDR-CD34-Mu-96Tests 96 Tests
EUR 742

CD34 antigen (CD34) polyclonal antibody

ABP-PAB-00010 100 ug Ask for price

CD34 Antibody

CSB-PA269036-
EUR 335
Description: A polyclonal antibody against CD34. Recognizes CD34 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB;WB:1:500-1:1000

CD34 Antibody

CSB-PA269036-100ul 100ul
EUR 316
Description: A polyclonal antibody against CD34. Recognizes CD34 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB;WB:1:500-1:1000

CD34 Antibody

1-CSB-PA001451
  • EUR 222.00
  • EUR 195.00
  • 100ug
  • 50ug
Description: A polyclonal antibody against CD34. Recognizes CD34 from Human, Mouse. This antibody is Unconjugated. Tested in the following application: WB, ELISA;WB:1/500-1/2000.ELISA:1/10000

CD34 Antibody

CSB-PA004926KA01HU-
EUR 335
Description: A polyclonal antibody against CD34. Recognizes CD34 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC;WB:1:500-1:2000, IHC:1:50-1:200

CD34 Antibody

CSB-PA004926KA01HU-100ul 100ul
EUR 389
Description: A polyclonal antibody against CD34. Recognizes CD34 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC;WB:1:500-1:2000, IHC:1:50-1:200

CD34 Antibody

1-CSB-PA10509A0Rb
  • EUR 317.00
  • EUR 335.00
  • 100ug
  • 50ug
Description: A polyclonal antibody against CD34. Recognizes CD34 from Human, Mouse. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC, IF; Recommended dilution: WB:1:2000-1:5000, IHC:1:20-1:200, IF:1:50-1:200

CD34 Antibody

21469-100ul 100ul
EUR 252

CD34 Antibody

21469-50ul 50ul
EUR 187

CD34 Antibody

35493-100ul 100ul
EUR 390

CD34 Antibody

35494-100ul 100ul
EUR 390

CD34 Antibody

20-abx242939
  • EUR 467.00
  • EUR 328.00
  • 100 ul
  • 50 ul

CD34 antibody

38153-100ul 100ul
EUR 252

CD34 Antibody

43792-100ul 100ul
EUR 252

CD34 Antibody

48740-100ul 100ul
EUR 333

CD34 Antibody

48740-50ul 50ul
EUR 239

CD34 Antibody

ABD6139 100 ug
EUR 438

CD34 Antibody

ABF4036 100 ug
EUR 438

CD34 Antibody

ABF5149 100 ug
EUR 438

CD34 Antibody

DF6139 200ul
EUR 304
Description: CD34 Antibody detects endogenous levels of total CD34.

CD34 Antibody

BF0423 200ul
EUR 376
Description: CD34 antibody detects endogenous levels of total CD34.

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