CD34 + stem cell counting using labeled immobilized anti-CD34 antibody onto magnetic nanoparticles and EasyCounter BC image cytometer

CD34 + stem cell counting using labeled immobilized anti-CD34 antibody onto magnetic nanoparticles and EasyCounter BC image cytometer

The power of immobilized conjugate anti-CD34+ monoclonal antibody-dR110 and free conjugate anti-CD45+ monoclonal antibody-ATTO620 to exactly enumerate CD34+ stem cells and CD45+ cells in apheresis samples had been evaluated. The conjugates anti-CD34+ antibody-dR110 and anti-CD45+– antibody-ATTO620 had been ready.
Functionalized magnetic nanoparticles (MNPs) had been synthesized. The anti-CD34+ antibody-dR110 conjugate was immobilized on the modified MNPs utilizing a carbodiimide methodology. The stem cell depend in thawed apheresis samples was decided utilizing the free and the immobilized conjugate anti-CD34+ antibody-dR110 on MNPs and a picture cell counter EasyCounter BC.
A better stem cell depend and extra correct outcomes had been obtained with the immobilized conjugate, as a result of a separation and focus of the stem cells certain to antibody-dR110 on MNPs by exterior magnet had been carried out. Coefficients of variation of CD34+ cell depend in apheresis samples, decided by EasyCounter BC, had been ranged from 5.5 to six.9% and people of CD45+ cell depend from 3.
Eight to 4.7%. The viability of CD34+ cells was excessive from 98.5 to 99.6%. It was discovered that correlation coefficient between the stream cytometer and automated cell counter, utilizing free anti-CD34+ antibody-dR110 was 0.94, and when utilizing immobilized anti-CD34+antibody-dR110 on MNPs, the correlation coefficient was 0.97.

Floor Coating of Polytetrafluoroethylene with Extracellular Matrix and Anti-CD34 Antibodies Facilitates Endothelialization and Inhibits Platelet Adhesion Below Sheer Stress.

Expanded polytetrafluoroethylene (ePTFE) polymers don’t help endothelialization due to nonconductive traits in direction of mobile attachment. Interior floor modification of the grafts can enhance endothelialization and enhance the long-term patency charge of the ePTFE vascular grafts.
Right here we reported a way of inner-surface modification of ePTFE vascular graft with extracellular matrix (ECM) and CD34 monoclonal antibodies (CD34 mAb) to stimulate the adhesion and proliferation of circulating endothelial progenitor cells on ePTFE graft to boost graft endothelialization.
The interior floor of ECM-coated ePTFE grafts had been linked with CD34 mAb within the presence of 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide/N-hydroxysuccinimide (EDC/NHS) resolution and the physicochemical properties, floor morphology, biocompatibility, and hemocompatibility of the grafts had been studied.
The hydrophilicity of CD34 mAb-coated graft interior floor was considerably improved. Fourier remodel infrared spectroscopy evaluation confirmed ECM and CD34 mAb cross-linking within the ePTFE vascular grafts with our methodology. Scanning electron microscopy evaluation confirmed protein layer masking uniformly on the interior floor of the modified grafts.
The cell-counting kit-8 (CCK-8) assay confirmed that the modified graft has no apparent cytotoxicity. The modified graft confirmed a low hemolytic charge (0.9%) within the direct contact hemolysis check, suggesting the modification improved hemocompatibility of biopolymers. The modification additionally decreased adhesion of platelets, whereas considerably elevated the adhesion of endothelial cells on the grafts.
We conclude that our methodology permits ePTFE polymers modification with ECM and CD34 mAb, facilitates endothelialization, and inhibits platelet adhesion on the grafts, thus could enhance the long-term patency charge of the prosthetic bypass grafts.

CD34 antibody

10R-7653 50 ug
EUR 548.4
Description: Mouse monoclonal CD34 antibody

CD34 antibody

20R-2517 50 ug
EUR 269
Description: Rabbit polyclonal CD34 antibody

CD34 Antibody

21469 100ul
EUR 319

CD34 Antibody

21469-100ul 100ul
EUR 302.4

CD34 Antibody

21469-50ul 50ul
EUR 224.4

CD34 Antibody

35493 100ul
EUR 479

CD34 Antibody

35493-100ul 100ul
EUR 468

CD34 Antibody

35494 100ul
EUR 479

CD34 Antibody

35494-100ul 100ul
EUR 468

CD34 antibody

38153 100ul
EUR 439

CD34 antibody

38153-100ul 100ul
EUR 302.4

CD34 Antibody

48740 100ul
EUR 499

CD34 Antibody

48740-100ul 100ul
EUR 399.6

CD34 Antibody

48740-50ul 50ul
EUR 286.8

CD34 Antibody

E043792 100μg/100μl
EUR 255
Description: Available in various conjugation types.

CD34 Antibody

E021469 100μg/100μl
EUR 255
Description: Available in various conjugation types.

CD34 Antibody

E18-4036-1 50μg/50μl
EUR 145
Description: Available in various conjugation types.

CD34 Antibody

E18-4036-2 100μg/100μl
EUR 225
Description: Available in various conjugation types.

CD34 Antibody

E18-5149-1 50μg/50μl
EUR 145
Description: Available in various conjugation types.

CD34 Antibody

E18-5149-2 100μg/100μl
EUR 225
Description: Available in various conjugation types.

CD34 Antibody

E19-6139 100μg/100μl
EUR 225
Description: Available in various conjugation types.

CD34 Antibody

E10-20126 100μg/100μl
EUR 225
Description: Available in various conjugation types.

CD34 Antibody

F40213-0.08ML 0.08 ml
EUR 140.25
Description: CD34 is a monomeric cell surface antigen selectively expressed on human hematopoietic progenitor cells.

CD34 Antibody

F40213-0.4ML 0.4 ml
EUR 330.65
Description: CD34 is a monomeric cell surface antigen selectively expressed on human hematopoietic progenitor cells.

CD34 Antibody

F40831-0.08ML 0.08 ml
EUR 140.25
Description: CD34 is a monomeric cell surface antigen with a molecular mass of approximately 110 kD that is selectively expressed on human hematopoietic progenitor cells.

CD34 Antibody

F40831-0.4ML 0.4 ml
EUR 322.15
Description: CD34 is a monomeric cell surface antigen with a molecular mass of approximately 110 kD that is selectively expressed on human hematopoietic progenitor cells.

CD34 Antibody

E220098-1 50μg/50μl
EUR 145
Description: Available in various conjugation types.

CD34 Antibody

E220098-2 100μg/100μl
EUR 225
Description: Available in various conjugation types.

CD34 Antibody

E80108-2 100μl
EUR 225
Description: Available in various conjugation types.

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