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CRISPR-Cas systems are widespread accessory elements across bacterial and archaeal plasmids
Many prokaryotes encode CRISPR-Cas programs as immune safety in opposition to cellular genetic parts (MGEs), but a variety of MGEs additionally harbor CRISPR-Cas parts. With a couple of exceptions, CRISPR-Cas loci encoded on MGEs are uncharted and a complete evaluation of their distribution, prevalence, variety, and performance is missing.
Right here, we systematically investigated CRISPR-Cas loci throughout the most important curated assortment of pure bacterial and archaeal plasmids. CRISPR-Cas loci are broadly however heterogeneously distributed throughout plasmids and, compared to host chromosomes, their imply prevalence per Mbp is increased and their distribution is distinct.
Moreover, the spacer content material of plasmid CRISPRs displays a robust focusing on bias in the direction of different plasmids, whereas chromosomal arrays are enriched with virus-targeting spacers. These contrasting focusing on preferences spotlight the genetic independence of plasmids and recommend a serious position for mediating plasmid-plasmid conflicts. Altogether, CRISPR-Cas are frequent accent parts of many plasmids, which is an neglected phenomenon that probably facilitates their dissemination throughout microbiomes.
Focused-antibacterial-plasmids (TAPs) combining conjugation and CRISPR/Cas programs obtain strain-specific antibacterial exercise
The worldwide emergence of drug-resistant micro organism results in the lack of efficacy of our antibiotics arsenal and severely limits the success of presently out there remedies. Right here, we developed an modern technique primarily based on targeted-antibacterial-plasmids (TAPs) that use bacterial conjugation to ship CRISPR/Cas programs exerting a strain-specific antibacterial exercise.
TAPs are extremely versatile as they are often directed in opposition to any particular genomic or plasmid DNA utilizing the customized algorithm (CSTB) that identifies applicable focusing on spacer sequences. We show the power of TAPs to induce strain-selective killing by introducing deadly double strand breaks (DSBs) into the focused genomes.
TAPs directed in opposition to a plasmid-born carbapenem resistance gene effectively resensitise the pressure to the drug. This work represents a necessary step towards the event of a substitute for antibiotic remedies, which might be used for in situ microbiota modification to eradicate focused resistant and/or pathogenic micro organism with out affecting different non-targeted bacterial species.
CRISPR–Cas and Restriction-Modification Act Additively in opposition to Conjugative Antibiotic Resistance Plasmid Switch in Enterococcus faecalis.
Enterococcus faecalis is an opportunistic pathogen and a number one reason for nosocomial infections. Conjugative pheromone-responsive plasmids are narrow-host-range cellular genetic parts (MGEs) which might be speedy disseminators of antibiotic resistance within the faecalis species.
Clustered frequently interspaced brief palindromic repeat (CRISPR)-Cas and restriction-modification confer acquired and innate immunity, respectively, in opposition to MGE acquisition in micro organism. Most multidrug-resistant E. faecalis isolates lack CRISPR-Cas and possess an orphan locus missing cas genes, CRISPR2, that’s of unknown operate.
Little is thought about restriction-modification protection in E. faecalis. Right here, we discover the speculation that multidrug-resistant E. faecalis strains are immunocompromised. We assessed MGE acquisition by E. faecalis T11, a pressure intently associated to the multidrug-resistant hospital isolate V583 however which lacks the ~620 kb of horizontally acquired genome content material that characterizes V583.
T11 possesses the E. faecalis CRISPR3-cas locus and a predicted restriction-modification system, neither of which happens in V583. We show that CRISPR-Cas and restriction-modification collectively confer a 4-log discount in acquisition of the pheromone-responsive plasmid pAM714 in biofilm matings.
Moreover, we present that the orphan CRISPR2 locus is purposeful for genome protection in opposition to one other pheromone-responsive plasmid, pCF10, solely within the presence of cas9 derived from the E. faecalis CRISPR1-cas locus, which most multidrug-resistant E. faecalis isolates lack.
Total, our work demonstrated that the lack of solely two loci led to a dramatic discount in genome protection in opposition to a clinically related MGE, highlighting the vital significance of the E. faecalis accent genome in modulating horizontal gene switch.
Our outcomes rationalize the event of antimicrobial methods that capitalize upon the immunocompromised standing of multidrug-resistant E. faecalis. IMPORTANCE Enterococcus faecalis is a bacterium that usually inhabits the gastrointestinal tracts of people and different animals. Though these micro organism are members of our native intestine flora, they’ll trigger life-threatening infections in hospitalized sufferers.
Antibiotic resistance genes seem like readily shared amongst high-risk E. faecalis strains, and multidrug resistance in these micro organism limits remedy choices for infections. Right here, we discover that CRISPR-Cas and restriction-modification programs, which operate as adaptive and innate immune programs in micro organism, considerably impression the unfold of antibiotic resistance genes in E. faecalis populations. The lack of these programs in high-risk E. faecalis means that they’re immunocompromised, a tradeoff that permits them to readily purchase new genes and adapt to new antibiotics.
CRISPR–Cas focused plasmid integration into mammalian cells through non-homologous finish becoming a member of.
Mammalian cells are broadly used for the manufacturing of therapeutic recombinant proteins, as these cells facilitate correct folding and post-translational modifications typically important for optimum exercise. Focused insertion of a plasmid harboring a gene of curiosity into the genome of mammalian cells for the expression of a desired protein is a key step in manufacturing of such biologics.
Right here we present {that a} web site particular double strand break (DSB) generated each within the genome and the donor plasmid utilizing the CRISPR-Cas9 system could be effectively used to focus on ∼5 kb plasmids into mammalian genomes through nonhomologous finish becoming a member of (NHEJ).
We have been capable of obtain efficiencies of as much as 0.17% in HEK293 cells and 0.45% in CHO cells. This method holds promise for fast and environment friendly insertion of a big international DNA sequence right into a predetermined genomic web site in mammalian cells.
Feasibility for a big scale mouse mutagenesis by injecting CRISPR/Cas plasmid into zygotes.
The recombinant clustered regulatory interspaced brief palindromic repeats (CRISPR)/Cas system has opened a brand new period for mammalian genome modifying. Right here, we constructed pX330 plasmids expressing humanized Cas9 (hCas9) and single information RNAs (sgRNAs) in opposition to mouse genes and validated them each in vitro and in vivo.
Once we randomly selected 291 goal sequences inside protein coding areas of 73 genes, a median variety of off-target candidates (precise match 13 nucleotides from 3′ goal and NGG) discovered by Bowtie software program was 9.2 ± 21.0 (~1.Eight instances greater than the estimated worth, 5.2). We subsequent validated their exercise by observing inexperienced fluorescence reconstituted by homology dependent restore (HDR) of an EGFP expression cassette in HEK293T cells.
Of the pX330 plasmids examined, 81.8% (238/291) have been discovered to be purposeful in vitro. We lastly injected the validated pX330 plasmids into mouse zygotes in its round type in opposition to 32 genes (together with two genes beforehand examined) and obtained mutant mice at a 52.9 ± 22.3% (100/196) mutation frequency. Among the many pups carrying mutations on the autosomes, 43.6% (47/96) carried the mutations in each alleles.
CAS Blocking Peptide |
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| AF9032-BP | Affbiotech | 1mg | EUR 234 |
p130 Cas Antibody |
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| AF4748 | Affbiotech | 200ul | EUR 540 |
p130 Cas Antibody |
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| AF6350 | Affbiotech | 200ul | EUR 420 |
p130 Cas Antibody |
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| AF2675 | Affbiotech | 100ul | EUR 420 |
CAS Polyclonal Antibody |
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| ES1844-100ul | ELK Biotech | 100ul | EUR 334.8 |
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Description: A Rabbit Polyclonal antibody against CAS from Human. This antibody is tested and validated for WB, ELISA, IHC, IP, IF, WB, ELISA |
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CAS Polyclonal Antibody |
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| ES1844-50ul | ELK Biotech | 50ul | EUR 248.4 |
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Description: A Rabbit Polyclonal antibody against CAS from Human. This antibody is tested and validated for WB, ELISA, IHC, IP, IF, WB, ELISA |
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p130 Cas antibody |
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| 70R-31036 | Fitzgerald | 100 ug | EUR 392.4 |
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Description: Rabbit polyclonal p130 Cas antibody |
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CAS antibody (Tyr165) |
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| 70R-31498 | Fitzgerald | 100 ug | EUR 392.4 |
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Description: Rabbit polyclonal CAS antibody (Tyr165) |
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CAS antibody (Tyr249) |
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| 70R-31500 | Fitzgerald | 100 ug | EUR 392.4 |
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Description: Rabbit polyclonal CAS antibody (Tyr249) |
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CAS Polyclonal Antibody |
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| 40671-100ul | SAB | 100ul | EUR 302.4 |
CAS Polyclonal Antibody |
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| 40671-50ul | SAB | 50ul | EUR 224.4 |
p130 Cas Antibody |
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| 20-abx012761 | Abbexa |
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p130 Cas Antibody |
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| ABF6350 | Lifescience Market | 100 ug | EUR 525.6 |
Anti-CAS Antibody |
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| A04496 | BosterBio | 100ul | EUR 476.4 |
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Description: Rabbit Polyclonal Antibody for CAS Antibody (CSE1L) detection.tested for IP, WB in Human. |
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Anti-CAS antibody |
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| STJ92006 | St John's Laboratory | 200 µl | EUR 236.4 |
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Description: Rabbit polyclonal to CAS. |
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p130 Cas Blocking Peptide |
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| AF7826-BP | Affbiotech | 1mg | EUR 234 |
p130 Cas Blocking Peptide |
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| AF7827-BP | Affbiotech | 1mg | EUR 234 |
p130 Cas Blocking Peptide |
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| AF4748-BP | Affbiotech | 1mg | EUR 234 |
p130 Cas Blocking Peptide |
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| AF6350-BP | Affbiotech | 1mg | EUR 234 |
CAS Polyclonal Conjugated Antibody |
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| C40671 | SAB | 100ul | EUR 476.4 |
p130 Cas Polyclonal Antibody |
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| ES3093-100ul | ELK Biotech | 100ul | EUR 334.8 |
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Description: A Rabbit Polyclonal antibody against p130 Cas from Human/Mouse/Rat. This antibody is tested and validated for WB, ELISA, IHC, WB, ELISA |
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p130 Cas Polyclonal Antibody |
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| ES3093-50ul | ELK Biotech | 50ul | EUR 248.4 |
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Description: A Rabbit Polyclonal antibody against p130 Cas from Human/Mouse/Rat. This antibody is tested and validated for WB, ELISA, IHC, WB, ELISA |
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p130 Cas Polyclonal Antibody |
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| ES3094-100ul | ELK Biotech | 100ul | EUR 334.8 |
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Description: A Rabbit Polyclonal antibody against p130 Cas from Human/Mouse/Rat. This antibody is tested and validated for WB, ELISA, IHC, WB, ELISA |
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p130 Cas Polyclonal Antibody |
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| ES3094-50ul | ELK Biotech | 50ul | EUR 248.4 |
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Description: A Rabbit Polyclonal antibody against p130 Cas from Human/Mouse/Rat. This antibody is tested and validated for WB, ELISA, IHC, WB, ELISA |
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p130 Cas antibody (Tyr410) |
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| 70R-31035 | Fitzgerald | 100 ug | EUR 392.4 |
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Description: Rabbit polyclonal p130 Cas antibody (Tyr410) |
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AE-1477 EzBlock CAS |
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| 2332617 | Atto | 3unit | EUR 352.8 |
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Description: Casei n for wer st er n bl ot t i ng |
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p130 Cas (pY410) Antibody |
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| abx010316-100ug | Abbexa | 100 ug | EUR 526.8 |
p130 Cas (pT249) Antibody |
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| 20-abx009713 | Abbexa |
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p130 Cas Polyclonal Antibody |
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| ABP52094-003ml | Abbkine | 0.03ml | EUR 189.6 |
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Description: A polyclonal antibody for detection of p130 Cas from Human, Mouse, Rat. This p130 Cas antibody is for WB, IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human p130 Cas around the non-phosphorylation site of Y165 |
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p130 Cas Polyclonal Antibody |
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| ABP52094-01ml | Abbkine | 0.1ml | EUR 346.8 |
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Description: A polyclonal antibody for detection of p130 Cas from Human, Mouse, Rat. This p130 Cas antibody is for WB, IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human p130 Cas around the non-phosphorylation site of Y165 |
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p130 Cas Polyclonal Antibody |
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| ABP52094-02ml | Abbkine | 0.2ml | EUR 496.8 |
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Description: A polyclonal antibody for detection of p130 Cas from Human, Mouse, Rat. This p130 Cas antibody is for WB, IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human p130 Cas around the non-phosphorylation site of Y165 |
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p130 Cas Polyclonal Antibody |
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| ABP52095-003ml | Abbkine | 0.03ml | EUR 189.6 |
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Description: A polyclonal antibody for detection of p130 Cas from Human, Mouse, Rat. This p130 Cas antibody is for WB, IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human p130 Cas around the non-phosphorylation site of Y410 |
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p130 Cas Polyclonal Antibody |
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| ABP52095-01ml | Abbkine | 0.1ml | EUR 346.8 |
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Description: A polyclonal antibody for detection of p130 Cas from Human, Mouse, Rat. This p130 Cas antibody is for WB, IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human p130 Cas around the non-phosphorylation site of Y410 |
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p130 Cas Polyclonal Antibody |
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| ABP52095-02ml | Abbkine | 0.2ml | EUR 496.8 |
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Description: A polyclonal antibody for detection of p130 Cas from Human, Mouse, Rat. This p130 Cas antibody is for WB, IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human p130 Cas around the non-phosphorylation site of Y410 |
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Anti-p130 Cas antibody |
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| STJ94853 | St John's Laboratory | 200 µl | EUR 236.4 |
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Description: Rabbit polyclonal to p130 Cas. |
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Anti-p130 Cas antibody |
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| STJ94854 | St John's Laboratory | 200 µl | EUR 236.4 |
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Description: Rabbit polyclonal to p130 Cas. |
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Donkey Anti-Sheep IgG, Salt Fractionation (Bulk) |
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| 30326-CAS-BLK | Alpha Diagnostics | 1g; bulk | Ask for price |
Phospho-p130 Cas (Tyr410) Antibody |
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| AF3350 | Affbiotech | 200ul | EUR 420 |
Phospho-p130 Cas (Tyr165) Antibody |
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| AF4448 | Affbiotech | 200ul | EUR 540 |
Phospho-p130 Cas (Tyr267) Antibody |
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| AF7326 | Affbiotech | 200ul | EUR 540 |
Phospho-p130 Cas (Tyr387) Antibody |
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| AF7327 | Affbiotech | 200ul | EUR 540 |
Phospho-p130 Cas (Tyr249) Antibody |
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| AF2375 | Affbiotech | 200ul | EUR 420 |
Statine *CAS 49642-07-1* |
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| 5109 | AAT Bioquest | 100 mg | EUR 262.8 |
Coelenterazine *CAS#: 55779-48-1* |
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| 21150 | AAT Bioquest | 250 ug | EUR 138 |
Coelenterazine *CAS#: 55779-48-1* |
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| 21156 | AAT Bioquest | 1 mg | EUR 158.4 |
Coelenterazine *CAS#: 55779-48-1* |
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| 21166 | AAT Bioquest | 10 mg | EUR 576 |
Zinquin *CAS#: 151606-29-0* |
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| 21260 | AAT Bioquest | 5 mg | EUR 158.4 |
Hydroxystilbamidine *CAS 223769-64-0* |
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| 17514 | AAT Bioquest | 10 mg | EUR 158.4 |
When off-target candidate websites have been examined in 63 mutant mice, 0.8% (3/382) have been mutated. We conclude that our methodology gives a easy, environment friendly, and cost-effective method for mammalian gene modifying that’s relevant for giant scale mutagenesis in mammals.
