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Multiplex TaqMan Real-Time PCR Assay for Sensitive Detection of Two Weevil Species (Coleoptera: Curculionidae)

Can quantitative RT-PCR for SARS-CoV-2 help in better management of patients and control of coronavirus disease 2019 pandemic

The emergence of SARS-CoV-2, the causative agent of coronavirus disease 2019 (COVID-19), represents a public health emergency of unprecedented proportion. The global containment efforts have been focused on testing, tracing of contacts and treatment (isolation) of those found COVID-19 positive. Since the whole genome sequences of a number of strains of this novel RNA virus were available in the public domain by early January 2020, a number of real-time polymerase chain reaction (RT-PCR) protocols were designed and used for diagnosis of this infection.
Most RT-PCRs are designed for qualitative COVID-19 reporting (SARS-CoV-2 detected or not detected), but have been used for semi-quantitative estimation of viral load based on cycle threshold value. Our manuscript discusses the utility of quantitative PCR testing for COVID-19 and its patient management benefits.

Multiplex TaqMan Real-Time PCR Assay for Sensitive Detection of Two Weevil Species (Coleoptera: Curculionidae)

Rapid and cost-efficient identification of Naupactus species is becoming a key process for the exportation of citrus fruit from Chile and other countries, considering the quarantine regulations for some species of the cosmopolitan genus Naupactus.
This study deals with the development of a fast and sensitive detection protocol for Naupactus cervinus (Coleoptera: Curculionidae) (Boheman) and Naupactus xanthographus (Coleoptera: Curculionidae) (Germar) based on multiplex TaqMan Real-time polymerase chain reaction. Both N. cervinus and N. xanthographus primer and probe sets achieved species-specific detection in a linear range from 1 pg/μl to 1 × 10-6 pg/μl, allowing detection of as few as 160 copies of template DNA. Non-target amplifications were not detected and a panel composed of 480 test samples had 100% coincidence with the respective morphological identification.

Validation of swab sampling and SYBR Green-based real-time PCR for the diagnosis of Cutaneous Leishmaniasis in French Guiana

 

Recent studies have highlighted the interest of non-invasive sampling procedures coupled to real-time PCR methods for detection of Leishmania species in South America. In French Guiana, sampling method still relied on skin biopsies. Non-invasive protocols should be tested on a large annual cohort to improve routine laboratory diagnosis of Cutaneous Leishmaniasis.
Therefore, we evaluated the performances of a new Leishmania detection and species identification protocol involving cotton swabs and SYBR Green real-time PCR of Hsp70 gene, coupled with Sanger sequencing. Between May 2017 and May 2018, 145 patients with ulcerated lesions compatible with Cutaneous Leishmaniasis were included in the Cayenne Hospital and its remote health centres. Each patient underwent scrapings for smear, skin biopsies for parasite culture and PCR-RFLP (RNA pol II) and cotton swabs for SYBR Green PCR. The most accurate diagnostic test was the SYBR Green PCR on swab sampling, showing a 98% sensitivity.
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aprofarm

SYBR Green qPCR Master Mix (Low ROX)

HY-K0522 5 mL (500 rxns )
EUR 308.4

SYBR Green qPCR Master Mix (High ROX)

HY-K0521 1 mL (100 rxns)
EUR 135.6

2x SYBR Green qPCR Master Mix (Low ROX)

B21702 5 ml
EUR 268.8
Description: 2x SYBR Green qPCR master mix (Low ROX) utilizes a special performance-enhanced Taq DNA polymerase protected via a hot-start activation technique, and optimized qPCR buffer system to perform SYBR

2x SYBR Green qPCR Master Mix (Low ROX)

B21703 25 ml
EUR 1027.2
Description: 2x SYBR Green qPCR master mix (Low ROX) utilizes a special performance-enhanced Taq DNA polymerase protected via a hot-start activation technique, and optimized qPCR buffer system to perform SYBR

2x SYBR Green qPCR Master Mix (High ROX)

B21402 5 ml
EUR 268.8
Description: 2x SYBR Green qPCR master mix (High ROX) utilizes a special performance-enhanced Taq DNA polymerase protected via a hot-start activation technique, and optimized qPCR buffer system to perform SYBR

2x SYBR Green qPCR Master Mix (High ROX)

B21403 25 ml
EUR 1027.2
Description: 2x SYBR Green qPCR master mix (High ROX) utilizes a special performance-enhanced Taq DNA polymerase protected via a hot-start activation technique, and optimized qPCR buffer system to perform SYBR

AceQ qPCR SYBR® Green Master Mix

Q111-02 500 rxn (20 μl/rxn)
EUR 265.2

AceQ qPCR SYBR® Green Master Mix

Q111-03 2500 rxn (20 μl/rxn)
EUR 775.2

ChamQ SYBR qPCR Master Mix

Q311-02 500 rxns (20 μl/rxn)
EUR 96.44

ChamQ SYBR qPCR Master Mix

Q311-03 2,500 rxns (20 μl/rxn)
EUR 434.8

ChamQ SYBR Color qPCR Master Mix

Q411-02 500 rxns (20 μl/rxn)
EUR 112.5

ChamQ SYBR Color qPCR Master Mix

Q411-03 2,500 rxns (20 μl/rxn)
EUR 507.2

AceQ Universal SYBR qPCR Master Mix

Q511-02 500 rxn (20 μl/rxn)
EUR 265.2

AceQ Universal SYBR qPCR Master Mix

Q511-03 2500 rxn (20 μl/rxn)
EUR 775.2

ChamQ Universal SYBR qPCR Master Mix

Q711-02 500 rxn (20 μl/rxn)
EUR 265.2

ChamQ Universal SYBR qPCR Master Mix

Q711-03 2500 rxn (20 μl/rxn)
EUR 775.2

ChamQ SYBR qPCR Master Mix (Without ROX)

Q321-02 500 rxns (20 μl/rxn)
EUR 96.44

ChamQ SYBR qPCR Master Mix (Without ROX)

Q321-03 2,500 rxns (20 μl/rxn)
EUR 434.8

Taq Pro Universal SYBR qPCR Master Mix

Q712-02 500 rxns (20 μl/rxn)
EUR 128.8

Taq Pro Universal SYBR qPCR Master Mix

Q712-03 2,500 rxns (20 μl/rxn)
EUR 580

miRNA Universal SYBR® qPCR Master Mix

MQ101-01 125 rxn(20 μl/rxn)
EUR 165.6

miRNA Universal SYBR® qPCR Master Mix

MQ101-02 500 rxn(20 μl/rxn)
EUR 290.4

ChamQ SYBR qPCR Master Mix (Low ROX Premixed)

Q331-02 500 rxns (20 μl/rxn)
EUR 96.44

ChamQ SYBR qPCR Master Mix (Low ROX Premixed)

Q331-03 2,500 rxns (20 μl/rxn)
EUR 434.8

ChamQ SYBR qPCR Master Mix (High ROX Premixed)

Q341-02 500 rxns (20 μl/rxn)
EUR 96.44

ChamQ SYBR qPCR Master Mix (High ROX Premixed)

Q341-03 2,500 rxns (20 μl/rxn)
EUR 434.8

ChamQ SYBR Color qPCR Master Mix (Without ROX)

Q421-02 500 rxns (20 μl/rxn)
EUR 112.5

ChamQ SYBR Color qPCR Master Mix (Without ROX)

Q421-03 2,500 rxns (20 μl/rxn)
EUR 507.2

ChamQ SYBR Color qPCR Master Mix (Low ROX Premixed)

Q431-02 500 rxns (20 μl/rxn)
EUR 112.5

ChamQ SYBR Color qPCR Master Mix (Low ROX Premixed)

Q431-03 2,500 rxns (20 μl/rxn)
EUR 507.2

ChamQ SYBR Color qPCR Master Mix (High ROX Premixed)

Q441-02 500 rxns (20 μl/rxn)
EUR 112.5

ChamQ SYBR Color qPCR Master Mix (High ROX Premixed)

Q441-03 2,500 rxns (20 μl/rxn)
EUR 507.2

TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green *No ROX*

17270-1mL 1 mL
EUR 99
Description: TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR.

TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green *No ROX*

17271-5mL 5 mL
EUR 308
Description: TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR.

TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green *Low ROX*

17272-1mL 1 mL
EUR 99
Description: TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR.

TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green *Low ROX*

17273-5mL 5 mL
EUR 308
Description: TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR.

TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green *High ROX*

17274-1mL 1 mL
EUR 99
Description: TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR.

TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green *High ROX*

17275-5mL 5 mL
EUR 308
Description: TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR.

Genious 2X SYBR Green Fast qPCR Mix

RK21204 1 mL Ask for price

TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green *No ROX*

17276-1mL 1 mL
EUR 99
Description: TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR.

TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green *No ROX*

17277-5mL 5 mL
EUR 308
Description: TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR.

TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green *Low ROX*

17278-1mL 1 mL
EUR 99
Description: TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR.

TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green *Low ROX*

17279-5mL 5 mL
EUR 308
Description: TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR.

TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green *High ROX*

17280-1mL 1 mL
EUR 99
Description: TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR.

TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green *High ROX*

17281-5mL 5 mL
EUR 308
Description: TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR.

2X Universal SYBR Green Fast qPCR Mix

RK21203 1 mL Ask for price

TAQuest™ qPCR Master Mix with Helixyte™ Green *No ROX*

17270 1 mL
EUR 99

TAQuest™ qPCR Master Mix with Helixyte™ Green *No ROX*

17271 5 mL
EUR 308

TAQuest™ qPCR Master Mix with Helixyte™ Green *Low ROX*

17272 1 mL
EUR 99

TAQuest™ qPCR Master Mix with Helixyte™ Green *Low ROX*

17273 5 mL
EUR 308

TAQuest™ qPCR Master Mix with Helixyte™ Green *High ROX*

17274 1 mL
EUR 99

TAQuest™ qPCR Master Mix with Helixyte™ Green *High ROX*

17275 5 mL
EUR 308

TAQuest™ FAST qPCR Master Mix with Helixyte™ Green *No ROX*

17276 1 mL
EUR 99

TAQuest™ FAST qPCR Master Mix with Helixyte™ Green *No ROX*

17277 5 mL
EUR 308

lyophilised real-time PCR Master Mix with SYBR® Green fluorescent DNA stain

M-PCR-173L 960 reactions x 20 µl
EUR 897
Description: lyophilised real-time PCR Master Mix with SYBR® Green fluorescent DNA stain

lyophilised real-time PCR Master Mix with SYBR® Green fluorescent DNA stain

M-PCR-173S 192 reactions x 20 µl
EUR 224
Description: lyophilised real-time PCR Master Mix with SYBR® Green fluorescent DNA stain

TAQuest™ FAST qPCR Master Mix with Helixyte™ Green *Low ROX*

17278 1 mL
EUR 99

TAQuest™ FAST qPCR Master Mix with Helixyte™ Green *Low ROX*

17279 5 mL
EUR 308

TAQuest™ FAST qPCR Master Mix with Helixyte™ Green *High ROX*

17280 1 mL
EUR 99

TAQuest™ FAST qPCR Master Mix with Helixyte™ Green *High ROX*

17281 5 mL
EUR 308

Genious 2X SYBR GREEN FAST qPCR MIX (No ROX)

RK21205 1 ml Ask for price

amfiSure Ultra Fidelity PCR Master Mix(2X)

P0346-001 1ml
EUR 336

amfiSure Ultra Fidelity PCR Master Mix(2X)

P0346-002 2x1ml
EUR 578.4

amfiSure Ultra Fidelity PCR Master Mix(2X)

P0346-005 5x1ml
EUR 993.6

amfiSure Ultra Fidelity PCR Master Mix(2X)

P0346-010 10x1ml
EUR 1632

RT Master Mix for qPCR

HY-K0510 1 mL (100 rxns)
EUR 349.2

AceQ qPCR Probe Master Mix

Q112-02 500 rxn (20 μl/rxn)
EUR 265.2

AceQ qPCR Probe Master Mix

Q112-03 2500 rxn (20 μl/rxn)
EUR 775.2

HotStart PCR Master mix (2X, Green Dye)

BS9291 5X1mL, 5ml
EUR 251.4

HotStart PCR Master mix (2X, Green Dye)

BS9292 1ml
EUR 115.68

Entrans 2X qPCR Probe Master Mix

RK21208 40 RXN Ask for price

Fast Plus EvaGreen qPCR Master Mix (5000 rxn)

31020-2 50x1mL
EUR 4291.2
Description: Minimum order quantity: 1 unit of 50x1mL

Fast Plus EvaGreen qPCR Master Mix (500 rxn)

31020-1 5x1mL
EUR 524.4
Description: Minimum order quantity: 1 unit of 5x1mL

Green-2-Go qPCR Mastermix (500X20ul Rxn)

QPCR004-S 4X1.25ml
EUR 303.25

Fast EvaGreen Master Mix for qPCR (5000 rxn)

31003-2 50x1ML
EUR 3739.2
Description: Minimum order quantity: 1 unit of 50x1ML

Fast EvaGreen Master Mix for qPCR(200 rxn)

31003 2x1ML
EUR 244.8
Description: Minimum order quantity: 1 unit of 2x1ML

Fast EvaGreen Master Mix for qPCR (500 rxn)

31003-1 5x1ML
EUR 463.2
Description: Minimum order quantity: 1 unit of 5x1ML

Green-2-Go qPCR Mastermix-ROX (500X20ul Rxn)

QPCR001-R 4X1.25ml
EUR 303.25

Forget-Me-Notâ„¢ EvaGreen qPCR Master Mix (2000 reactions)

31041-20mL 2x10mL
EUR 592.8
Description: Minimum order quantity: 1 unit of 2x10mL

RT Master Mix for qPCR (gDNA digester plus)

HY-K0511 1 mL (100 rxns)
EUR 378

Green-2-Go qPCR Mastermix-iCycler (500X20ul Rxn)

QPCR003-iC 4X1.25ml
EUR 303.25

Forget-Me-Notâ„¢ EvaGreen qPCR Master Mix (500 reactions)

31041-1 5x1mL
EUR 326.4
Description: Minimum order quantity: 1 unit of 5x1mL

Forget-Me-Notâ„¢ EvaGreen qPCR Master Mix (100 reactions)

31041-T 1mL
EUR 136.8
Description: Minimum order quantity: 1 unit of 1mL

Genorise® 2 x qPCR HotStart Master Mix

GR108002 2.0 ml
EUR 279

Green-2-Go qPCR Mastermix-no Dye (500X20ul Rxn)

QPCR006-NODYE 4X1.25ml
EUR 326.22

Green-2-Go qPCR Mastermix-Low ROX (500X20ul Rxn)

QPCR002-L 4X1.25ml
EUR 303.25

TAQuestâ„¢ qPCR Master Mix for TaqMan Probes *No ROX*

17282-1mL 1 mL
EUR 99
Description: TAQuestâ„¢ qPCR Master Mix for TaqMan Probes is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR compatible with TaqMan gene expression assays.

TAQuestâ„¢ qPCR Master Mix for TaqMan Probes *No ROX*

17283-5mL 5 mL
EUR 308
Description: TAQuestâ„¢ qPCR Master Mix for TaqMan Probes is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR compatible with TaqMan gene expression assays.

TAQuestâ„¢ qPCR Master Mix for TaqMan Probes *Low ROX*

17284-1mL 1 mL
EUR 99
Description: TAQuestâ„¢ qPCR Master Mix for TaqMan Probes is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR compatible with TaqMan gene expression assays.

TAQuestâ„¢ qPCR Master Mix for TaqMan Probes *Low ROX*

17285-5mL 5 mL
EUR 308
Description: TAQuestâ„¢ qPCR Master Mix for TaqMan Probes is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR compatible with TaqMan gene expression assays.

TAQuestâ„¢ qPCR Master Mix for TaqMan Probes *High ROX*

17286-1mL 1 mL
EUR 99
Description: TAQuestâ„¢ qPCR Master Mix for TaqMan Probes is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR compatible with TaqMan gene expression assays.

TAQuestâ„¢ qPCR Master Mix for TaqMan Probes *High ROX*

17287-5mL 5 mL
EUR 308
Description: TAQuestâ„¢ qPCR Master Mix for TaqMan Probes is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR compatible with TaqMan gene expression assays.

TAQuest™ qPCR Master Mix for TaqMan Probes *No ROX*

17282 1 mL
EUR 99

TAQuest™ qPCR Master Mix for TaqMan Probes *No ROX*

17283 5 mL
EUR 308

TAQuest™ qPCR Master Mix for TaqMan Probes *Low ROX*

17284 1 mL
EUR 99

TAQuest™ qPCR Master Mix for TaqMan Probes *Low ROX*

17285 5 mL
EUR 308

Fast Plus EvaGreen qPCR Master Mix (trial size, 100 rxn)

31020-T 1mL
EUR 160.8
Description: Minimum order quantity: 1 unit of 1mL

TAQuest™ qPCR Master Mix for TaqMan Probes *High ROX*

17286 1 mL
EUR 99

TAQuest™ qPCR Master Mix for TaqMan Probes *High ROX*

17287 5 mL
EUR 308

TAQuestâ„¢ FAST qPCR Master Mix for TaqMan Probes *No ROX*

17288-1mL 1 mL
EUR 99
Description: TAQuestâ„¢ FAST qPCR Master Mix for TaqMan Probes is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR well suited to use for TaqMan gene expression assays.

TAQuestâ„¢ FAST qPCR Master Mix for TaqMan Probes *No ROX*

17289-5mL 5 mL
EUR 308
Description: TAQuestâ„¢ FAST qPCR Master Mix for TaqMan Probes is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR well suited to use for TaqMan gene expression assays.

Green-2-Go TaqProbe qPCR Mastermix-no Dye (500X20ul Rxn)

QPCR005-NODYE 4X1.25ml
EUR 303.25

TAQuestâ„¢ FAST qPCR Master Mix for TaqMan Probes *Low ROX*

17290-1mL 1 mL
EUR 99
Description: TAQuestâ„¢ FAST qPCR Master Mix for TaqMan Probes is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR well suited to use for TaqMan gene expression assays.

TAQuestâ„¢ FAST qPCR Master Mix for TaqMan Probes *Low ROX*

17291-5mL 5 mL
EUR 308
Description: TAQuestâ„¢ FAST qPCR Master Mix for TaqMan Probes is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR well suited to use for TaqMan gene expression assays.

TAQuestâ„¢ FAST qPCR Master Mix for TaqMan Probes *High ROX*

17292-1mL 1 mL
EUR 99
Description: TAQuestâ„¢ FAST qPCR Master Mix for TaqMan Probes is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR well suited to use for TaqMan gene expression assays.

TAQuestâ„¢ FAST qPCR Master Mix for TaqMan Probes *High ROX*

17293-5mL 5 mL
EUR 308
Description: TAQuestâ„¢ FAST qPCR Master Mix for TaqMan Probes is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR well suited to use for TaqMan gene expression assays.

Fast Plus EvaGreen qPCR Master Mix with low Rox (500 rxn)

31014-1 5x1mL
EUR 524.4
Description: Minimum order quantity: 1 unit of 5x1mL

Fast Plus EvaGreen qPCR Master Mix with low Rox (5000 rxn)

31014-2 50x1mL
EUR 4291.2
Description: Minimum order quantity: 1 unit of 50x1mL

Fast Plus EvaGreen qPCR Master Mix with high Rox (500 rxn)

31015-1 5x1mL
EUR 524.4
Description: Minimum order quantity: 1 unit of 5x1mL

Fast Plus EvaGreen qPCR Master Mix with high Rox (5000 rxn)

31015-2 50x1mL
EUR 4291.2
Description: Minimum order quantity: 1 unit of 50x1mL

TAQuest™ FAST qPCR Master Mix for TaqMan Probes *No ROX*

17288 1 mL
EUR 99

TAQuest™ FAST qPCR Master Mix for TaqMan Probes *No ROX*

17289 5 mL
EUR 308

qPCR Master Mix DLP1 (SafeLine: dUTP), packed in 1,25 ml vials

S190 100 rcs (2,5 ml)
EUR 165.6

qPCR Master Mix DLP1 (SafeLine: dUTP), packed in 1,25 ml vials

S190L 500 rcs (12,5 ml)
EUR 627.6

TAQuest™ FAST qPCR Master Mix for TaqMan Probes *Low ROX*

17290 1 mL
EUR 99

TAQuest™ FAST qPCR Master Mix for TaqMan Probes *Low ROX*

17291 5 mL
EUR 308

TAQuest™ FAST qPCR Master Mix for TaqMan Probes *High ROX*

17292 1 mL
EUR 99

TAQuest™ FAST qPCR Master Mix for TaqMan Probes *High ROX*

17293 5 mL
EUR 308

Fast EvaGreen Master Mix for qPCR, trial size (100 rxn)

31003-T 1ML
EUR 147.6
Description: Minimum order quantity: 1 unit of 1ML

Fast Plus EvaGreen qPCR Master Mix with low Rox (100 rxn): (1mL)

31014-T 1mL
EUR 160.8
Description: Minimum order quantity: 1 unit of 1mL
Mean PCR cycle threshold (Ct) was of 24.4 Ct (min=17 Ct, max=36 Ct) and was inferior to 35 Ct in 97.6% of samples. All samples positive with real-time PCR SYBR Green were successfully identified at the species level by DNA sequencing. This new method should be considered for routine diagnosis of Cutaneous Leishmaniasis in South America and especially for remote areas, as non-invasive collection tools are easier to use and require less precautions for transportation.

Development and clinical validation of a pangenotypic PCR-based assay for the detection and quantification of hepatitis E virus (Orthopevirus A genus)

 

The objective of this study was to design a pangenotypic PCR-based assay for the detection and quantification of hepatitis E virus (HEV) RNA from across the entire spectrum of described genotypes belonging to the Orthohepevirus A genus. The optimal conditions and the performance of the assay were determined by testing the WHO standard strain (6219/10) and the WHO HEV panel (8578/13).
Similarly, performance comparisons were made with two commercial assays (Real Star HEV RT-PCR 2.0 and ampliCube HEV 2.0 Quant) to detect HEV RNA at concentrations below 1,000 IU/mL with viral strains from the WHO and to test samples from 54 patients with acute hepatitis. The assay presented in this study was able to detect the entire spectrum of described genotypes belonging to the Orthohepevirus A genus, demonstrating a better performance than both commercial kits. This procedure may represent a significant improvement in the molecular diagnosis of HEV infection.

SARS-CoV-2 and RT-PCR in asymptomatic patients: Results of a cohort of workers at El Dorado International Airport in Bogotá, 2020

 

Introduction: The 2019 coronavirus pandemic (COVID-19) has caused around 25 million cases worldwide. Asymptomatic patients have been described as potential sources of transmission. However, there are difficulties to detect them and to establish their role in the dynamics of virus transmission, which hinders the implementation of prevention strategies. Objective: To describe the behavior of asymptomatic SARS-CoV-2 virus infection in a cohort of workers at the El Dorado “Luis Carlos Galán Sarmiento” International Airport in Bogotá, Colombia. Materials and methods: A prospective cohort of 212 workers from the El Dorado airport was designed. The follow-up began in June, 2020. A survey was used to characterize health and work conditions.
Every 21 day, a nasopharyngeal swab was taken to identify the presence of SARS-CoV-2 using RT-PCR. We analyzed the behavior of the cycle threshold (ORF1ab and N genes) according to the day of follow-up. Results: In the first three follow-ups of the cohort, we found an incidence of SARS-CoV-2 infection of 16.51%. The proportion of positive contacts was 14.08%. The median threshold for cycle threshold was 33.53. Conclusion: We characterized the asymptomatic SARS-CoV-2 infection in a cohort of workers. The identification of asymptomatic infected persons continues to be a challenge for epidemiological surveillance systems.
Introducción. La pandemia de COVID-19 ha ocasionado cerca de 25 millones de casos en el mundo. Se ha descrito que los pacientes asintomáticos pueden ser fuentes de transmisión. Sin embargo, es difícil detectarlos y no es claro su papel en la dinámica de transmisión del virus, lo que obstaculiza la implementación de estrategias para la prevención.
Objetivo. Describir el comportamiento de la infección asintomática por SARS-CoV-2 en una cohorte de trabajadores del Aeropuerto Internacional El Dorado “Luis Carlos Galán Sarmiento” de Bogotá, Colombia. Materiales y métodos. Se diseñó una cohorte prospectiva de trabajadores del Aeropuerto El Dorado. El seguimiento se inició en junio de 2020 con una encuesta a cada trabajador para caracterizar sus condiciones de salud y trabajo. Cada 21 días se tomó una muestra de hisopado nasofaríngeo para detectar la presencia del SARS-CoV-2 mediante reacción en cadena de la polimerasa con transcriptasa inversa (RT-PCR). Se analizó el comportamiento del umbral del ciclo (cycle threshold) de los genes ORF1ab y N según el día de seguimiento.
Resultados. En los primeros tres seguimientos de la cohorte se encontró una incidencia de la infección por SARS-CoV-2 del 16,51 %. La proporción de contactos positivos fue del 14,08 %. La mediana del umbral del ciclo fue de 33,53. Conclusión. Se determinaron las características de la infección asintomática por el SARSCoV-2 en una cohorte de trabajadores. La detección de infectados asintomáticos sigue siendo un reto para los sistemas de vigilancia epidemiológica.

qPCR ProbesMasterMaster mix for quantitative real-time PCR using labeled DNA probes, 2 x conc. master mix** shipped ice packs - must be shipped via overnight service

M-PCR-360S 2 x 1,25 ml
EUR 205
Description: qPCR ProbesMasterMaster mix for quantitative real-time PCR using labeled DNA probes, 2 x conc. master mix** shipped ice packs - must be shipped via overnight service

qPCR ProbesMaster UNGMaster mix for quantitative real-time PCR using labeled DNA probes, 2 x conc. master mix** shipped ice packs - must be shipped via overnight service

M-PCR-363L 10 x 1,25 ml
EUR 982
Description: qPCR ProbesMaster UNGMaster mix for quantitative real-time PCR using labeled DNA probes, 2 x conc. master mix** shipped ice packs - must be shipped via overnight service

qPCR ProbesMaster UNGMaster mix for quantitative real-time PCR using labeled DNA probes, 2 x conc. master mix** shipped ice packs - must be shipped via overnight service

M-PCR-363S 2 x 1,25 ml
EUR 246
Description: qPCR ProbesMaster UNGMaster mix for quantitative real-time PCR using labeled DNA probes, 2 x conc. master mix** shipped ice packs - must be shipped via overnight service

qPCR ProbesMaster highROXMaster mix for quantitative real-time PCR using labeled DNA probes, 2x conc. master mix** shipped ice packs - must be shipped via overnight service

M-PCR-362L 10 x 1,25 ml
EUR 818
Description: qPCR ProbesMaster highROXMaster mix for quantitative real-time PCR using labeled DNA probes, 2x conc. master mix** shipped ice packs - must be shipped via overnight service

qPCR ProbesMaster highROXMaster mix for quantitative real-time PCR using labeled DNA probes, 2x conc. master mix** shipped ice packs - must be shipped via overnight service

M-PCR-362S 2 x 1,25 ml
EUR 205
Description: qPCR ProbesMaster highROXMaster mix for quantitative real-time PCR using labeled DNA probes, 2x conc. master mix** shipped ice packs - must be shipped via overnight service

qPCR ProbesMaster lowROXMaster mix for quantitative real-time PCR using labeled DNA probes, 2 x conc. master mix** shipped ice packs - must be shipped via overnight service

M-PCR-361L 10 x 1,25 ml
EUR 818
Description: qPCR ProbesMaster lowROXMaster mix for quantitative real-time PCR using labeled DNA probes, 2 x conc. master mix** shipped ice packs - must be shipped via overnight service

qPCR ProbesMaster lowROXMaster mix for quantitative real-time PCR using labeled DNA probes, 2 x conc. master mix** shipped ice packs - must be shipped via overnight service

M-PCR-361S 2 x 1,25 ml
EUR 205
Description: qPCR ProbesMaster lowROXMaster mix for quantitative real-time PCR using labeled DNA probes, 2 x conc. master mix** shipped ice packs - must be shipped via overnight service

qPCR ProbesMaster UNG lowROXMaster mix for quantitative real-time PCR using labeled DNA probes, 2 x conc. master mix** shipped ice packs - must be shipped via overnight service

M-PCR-364L 10 x 1,25 ml
EUR 982
Description: qPCR ProbesMaster UNG lowROXMaster mix for quantitative real-time PCR using labeled DNA probes, 2 x conc. master mix** shipped ice packs - must be shipped via overnight service

qPCR ProbesMaster UNG lowROXMaster mix for quantitative real-time PCR using labeled DNA probes, 2 x conc. master mix** shipped ice packs - must be shipped via overnight service

M-PCR-364S 2 x 1,25 ml
EUR 246
Description: qPCR ProbesMaster UNG lowROXMaster mix for quantitative real-time PCR using labeled DNA probes, 2 x conc. master mix** shipped ice packs - must be shipped via overnight service

qPCR ProbesMaster UNG highROXMaster mix for quantitative real-time PCR using labeled DNA probes, 2 x conc. master mix** shipped ice packs - must be shipped via overnight service

M-PCR-365L 10 x 1,25 ml
EUR 982
Description: qPCR ProbesMaster UNG highROXMaster mix for quantitative real-time PCR using labeled DNA probes, 2 x conc. master mix** shipped ice packs - must be shipped via overnight service

qPCR ProbesMaster UNG highROXMaster mix for quantitative real-time PCR using labeled DNA probes, 2 x conc. master mix** shipped ice packs - must be shipped via overnight service

M-PCR-365S 2 x 1,25 ml
EUR 246
Description: qPCR ProbesMaster UNG highROXMaster mix for quantitative real-time PCR using labeled DNA probes, 2 x conc. master mix** shipped ice packs - must be shipped via overnight service

Tiamulin ELISA Kit, Quantitative

DEIA036 96T
EUR 1414.8
Description: This kit is intended for quantitative analysis of tiamulin residue in animal tissue (pork and chicken, etc).

Human RBP4 Quantitative ELISA Kit

LF-EK70030 1×96T
EUR 722.4

Rat C3 ELISA Kit, 96 tests, Quantitative

6230 1 kit
EUR 854.4

Cat IgG ELISA Kit, 96 tests, Quantitative

300-100-CGG 1 kit
EUR 854.4

Cat IgM ELISA kit 96 tests, Quantitative

300-170-CGM 1 kit
EUR 854.4

Cat IgA ELISA Kit, 96 tests, Quantitative

300-180-CGA 1 kit
EUR 854.4

Cat IgE ELISA Kit, 96 tests, Quantitative

300-190-CGE 1 kit
EUR 854.4

Dog IgG ELISA Kit, 96 tests, Quantitative

400-100-DGG 1 Kit
EUR 781.2

Dog IgM ELISA Kit, 96 tests, Quantitative

400-170-DGM 1 Kit
EUR 781.2

Dog IgA ELISA Kit, 96 tests, Quantitative

400-180-DGA 1 Kit
EUR 854.4

Dog IgE ELISA Kit, 96 tests, Quantitative

400-190-DGE 1 Kit
EUR 854.4

Rat IgG ELISA Kit, 96 tests, Quantitative

6420 1 kit
EUR 781.2

Rat IgE ELISA Kit, 96 tests, Quantitative

6470 1 kit
EUR 927.6

Rat IgM ELISA Kit, 96 tests, Quantitative

6480 1 Kit
EUR 854.4

Rat IgA ELISA kit 96 tests, Quantitative

6410-10 1 kit
EUR 607.2

Pig IgM ELISA Kit, 96 tests, Quantitative

9080 1 kit
EUR 854.4

Lactate ELISA Kit, 96 tests, Quantitative

10795 1 Kit
EUR 927.6

Rat VEGF ELISA Kit, 96 tests, quantitative

200-840-VER 1 kit
EUR 927.6

Goat IgG ELISA Kit, 96 tests, Quantitative

7520 1 kit
EUR 781.2

Goat IgM ELISA Kit, 96 tests, Quantitative

7530 1 kit
EUR 927.6

Goat IgA ELISA Kit, 96 tests, Quantitative

7540 1 kit
EUR 1000.8

Goat IgE ELISA Kit, 96 tests, Quantitative

7550 1 kit
EUR 1000.8

Rat IgG1 ELISA Kit, 96 tests, Quantitative

6430 1 Kit
EUR 854.4

Oxytocin ELISA Kit, 96 tests, Quantitative

10790 1 Kit
EUR 927.6

Human IgG ELISA Kit, 96 tests, Quantitative

1750 1 Kit
EUR 781.2

Human IgM ELISA Kit, 96 tests, Quantitative

1760 1 Kit
EUR 781.2

Human IgA ELISA Kit, 96 tests, Quantitative

1780 1 kit
EUR 781.2

Sheep IgG ELISA Kit, 96 tests, Quantitative

7620 1 Kit
EUR 781.2

Sheep IgM ELISA Kit, 96 tests, Quantitative

7630 1 kit
EUR 854.4

Sheep IgA ELISA Kit, 96 tests, Quantitative

7640 1 kit
EUR 1000.8

Sheep IgE ELISA Kit, 96 tests, Quantitative

7650 1 kit
EUR 1000.8

Horse IgA ELISA Kit, 96 tests, Quantitative

7720 1 kit
EUR 854.4

Horse IgG ELISA Kit, 96 tests, Quantitative

7730 1 kit
EUR 854.4

Horse IgM ELISA Kit, 96 tests, Quantitative

7740 1 kit
EUR 854.4

Horse IgE ELISA Kit, 96 tests, Quantitative

7750 1 kit
EUR 927.6

Camel IgG ELISA Kit, 96 tests, Quantitative

7820 1 kit
EUR 854.4

Mouse IgA ELISA Kit, 96 tests, Quantitative

6310 1 kit
EUR 781.2

Mouse IgG ELISA Kit, 96 tests, Quantitative

6320 1 kit
EUR 781.2

Mouse IgE ELISA Kit, 96 tests, Quantitative

6370 1 Kit
EUR 781.2

Mouse IgM ELISA Kit, 96 tests, Quantitative

6380 1 Kit
EUR 781.2

Rat IgG2a ELISA Kit, 96 tests, Quantitative

6440 1 Kit
EUR 578.4

Rat IgG2b ELISA Kit, 96 tests, Quantitative

6450 1 Kit
EUR 634.8

G. Pig IgA ELISA Kit, 96 tests, Quantitative

7410 1 kit
EUR 854.4

G. Pig IgG ELISA Kit, 96 tests, Quantitative

7420 1 kit
EUR 781.2

G. Pig IgM ELISA Kit, 96 tests, Quantitative

7430 1 kit
EUR 781.2

Mouse VEGF ELISA Kit, 96 tests, Quantitative

100-230-VEM 1 Kit
EUR 854.4

Vitamin A ELISA Kit, 96 tests, Quantitative

10760 1 Kit
EUR 927.6

Vitamin B1 ELISA Kit, 96 tests, Quantitative

10765 1 Kit
EUR 927.6

Vitamin C ELISA Kit, 96 tests, Quantitative

10770 1 Kit
EUR 927.6

Vitamin E ELISA Kit, 96 tests, Quantitative

10775 1 Kit
EUR 927.6

Bovine IgG ELISA Kit, 96 tests, Quantitative

8010 1 Kit
EUR 781.2

Turkey IgG ELISA Kit, 96 tests, Quantitative

8020 1 kit
EUR 781.2

Horse Fab2 ELISA Kit, 96 tests, Quantitative

7710-Fab 1 kit
EUR 854.4

Mouse IgG1 ELISA Kit, 96 tests, Quantitative

6330 1 kit
EUR 781.2

Mouse IgG3 ELISA Kit, 96 tests, Quantitative

6360 1 kit
EUR 781.2

Monkey IgA ELISA Kit, 96 tests, Quantitative

7010 1 Kit
EUR 854.4

Rabbit IgA ELISA Kit, 96 tests, Quantitative

6510 1 kit
EUR 927.6

Rabbit IgM ELISA Kit, 96 tests, Quantitative

6580 1 kit
EUR 708

Bovine IgA ELISA Kit, 96 tests, Quantitative

8075 1 Kit
EUR 854.4

Bovine IgM ELISA Kit, 96 tests, Quantitative

8080 1 Kit
EUR 854.4

Mouse CCL2 ELISA Kit, 96 tests, Quantitative

MCC-0L2-100 1 kit
EUR 578.4

Mouse CCL3 ELISA Kit, 96 tests, Quantitative

MCC-0L3-100 1 kit
EUR 607.2

Human CCL2 ELISA Kit, 96 tests, Quantitative

HCC-0L2-105 1 kit
EUR 578.4

Human CCL3 ELISA Kit, 96 tests, Quantitative

HCC-0L3-110 1 kit
EUR 578.4

Human CCL4 ELISA Kit, 96 tests, Quantitative

HCC-0L4-115 1 kit
EUR 578.4

Human CD93 ELISA Kit, 96 tests, Quantitative

HCD-093-120 1 kit
EUR 578.4

Protein-A ELISA Kit, 480 tests, Quantitative

800-110-PRA-5 1 pk
EUR 3714

Rat IL-13 ELISA Kit, 96 tests, Quantitative

RIL-013-100 1 kit
EUR 578.4

Vasopressin ELISA Kit, 96 tests, Quantitative

10800 1 Kit
EUR 927.6

Pig Insulin ELISA Kit, 96 tests, Quantitative

10420 1 Kit
EUR 927.6

Vitamin B12 ELISA kit, 96 tests, Quantitative

0340-B12 1 Kit
EUR 927.6

D-Lactate ELISA Kit, 96 tests, Quantitative

10845 1 Kit
EUR 927.6

Protein-A ELISA Kit, 96 tests, Quantitative

800-110-PRA 1 kit
EUR 927.6

Mouse IgG2a ELISA Kit, 96 tests, Quantitative

6340 1 kit
EUR 781.2

Mouse IgG2b ELISA Kit, 96 tests, Quantitative

6350 1 kit
EUR 781.2

Dog Albumin ELISA Kit, 96 tests, Quantitative

7000-50 1 kit
EUR 781.2

Chicken IgM ELISA Kit, 96 tests, Quantitative

6040 1 kit
EUR 781.2

Rat Albumin ELISA Kit, 96 tests, Quantitative

6400 1 kit
EUR 781.2

Mouse CXCL3 ELISA Kit, 96 tests, Quantitative

MCXC-003-100 1 kit
EUR 578.4

Human CXCL3 ELISA Kit, 96 tests, Quantitative

HCXC-003-100 1 kit
EUR 578.4

Human CXCL9 ELISA Kit, 96 tests, Quantitative

HCXC-090-100 1 kit
EUR 578.4

Mouse IL-2 ELISA Kit, 96 tests, Quantitative

MIL-002-105 1 kit
EUR 578.4

Mouse IL-4 ELISA Kit, 96 tests, Quantitative

MIL-004-110 1 kit
EUR 578.4

Mouse IL-5 ELISA Kit, 96 tests, Quantitative

MIL-005-115 1 kit
EUR 578.4

Mouse IL-9 ELISA Kit, 96 tests, Quantitative

MIL-009-120 1 kit
EUR 578.4

Human IL-4 ELISA Kit, 96 tests, Quantitative

HIL-004-105 1 kit
EUR 578.4

Human IL-5 ELISA Kit, 96 tests, Quantitative

HIL-005-110 1 kit
EUR 578.4

Human IL-9 ELISA Kit, 96 tests, Quantitative

HIL-009-115 1 kit
EUR 607.2

Human IL-8 ELISA Kit, 96 tests, Quantitative

HIL-080-170 1 kit
EUR 578.4

Rat IL-17A ELISA Kit, 96 tests, Quantitative

RIL-17A-100 1 kit
EUR 607.2

Homocysteine ELISA kit, 96 tests, Quantitative

0370-HCY 1 kit
EUR 927.6

Prostacyclin ELISA Kit, 96 tests, Quantitative

10785 1 Kit
EUR 927.6

Mouse Leptin ELISA Kit, 96 tests, Quantitative

100-130-LEM 1 Kit
EUR 854.4

Goat Insulin ELISA Kit, 96 tests, Quantitative

10730 1 Kit
EUR 927.6

Rat Glucagon ELISA Kit, 96 tests, Quantitative

9485 1 Kit
EUR 927.6

Human CXCL10 ELISA Kit, 96 tests, Quantitative

HCXC-010-100 1 kit
EUR 708

Human CXCL16 ELISA Kit, 96 tests, Quantitative

HCXC-016-100 1 kit
EUR 578.4

Bovine IL-4 ELISA Kit, 96 tests, Quantitative

BIL-004-100 1 kit
EUR 634.8

Mouse IL-10 ELISA Kit, 96 tests, Quantitative

MIL-010-125 1 kit
EUR 578.4

Mouse IL-13 ELISA Kit, 96 tests, Quantitative

MIL-013-135 1 kit
EUR 578.4

Mouse IL-21 ELISA Kit, 96 tests, Quantitative

MIL-021-155 1 kit
EUR 607.2

Mouse IL-22 ELISA Kit, 96 tests, Quantitative

MIL-022-160 1 kit
EUR 607.2

Mouse IL-23 ELISA Kit, 96 tests, Quantitative

MIL-023-165 1 kit
EUR 607.2

Mouse IL-25 ELISA Kit, 96 tests, Quantitative

MIL-025-170 1 kit
EUR 607.2

Mouse IL-27 ELISA Kit, 96 tests, Quantitative

MIL-027-175 1 kit
EUR 607.2

Mouse IL-33 ELISA Kit, 96 tests, Quantitative

MIL-033-180 1 kit
EUR 607.2

Human IL-10 ELISA Kit, 96 tests, Quantitative

HIL-010-120 1 kit
EUR 578.4

Human IL-13 ELISA Kit, 96 tests, Quantitative

HIL-013-130 1 kit
EUR 607.2

Human IL-15 ELISA Kit, 96 tests, Quantitative

HIL-015-135 1 kit
EUR 607.2

Human IL-21 ELISA Kit, 96 tests, Quantitative

HIL-021-145 1 kit
EUR 578.4

Human IL-22 ELISA Kit, 96 tests, Quantitative

HIL-022-150 1 kit
EUR 607.2

Human IL-23 ELISA Kit, 96 tests, Quantitative

HIL-023-155 1 kit
EUR 607.2

Human IL-27 ELISA Kit, 96 tests, Quantitative

HIL-027-160 1 kit
EUR 607.2

Human IL-29 ELISA Kit, 96 tests, Quantitative

HIL-029-165 1 kit
EUR 578.4

Human Estrone ELISA Kit, 96 tests, Quantitative

1925 1 kit
EUR 708

Rat Clusterin ELISA kit 96 tests, Quantitative

610-110-RCL 1 Kit
EUR 927.6

Rat Hemopexin ELISA kit 96 tests, Quantitative

610-710-HPX 1 Kit
EUR 927.6

Mouse Albumin ELISA Kit, 96 tests, Quantitative

6300 1 kit
EUR 854.4

Pig Myoglobin ELISA kit 96 tests, Quantitative

600-640-PMY 1 Kit
EUR 1000.8

Rat Myoglobin ELISA kit 96 tests, Quantitative

600-660-RMY 1 kit
EUR 1000.8

Human Glucose ELISA Kit, 96 tests, Quantitative

9575 1 Kit
EUR 927.6

Sheep IgG-Fc ELISA Kit, 96 tests, Quantitative

7615-Fc 1 kit
EUR 854.4

Horse IgG-Fc ELISA Kit, 96 tests, Quantitative

7715-Fc 1 kit
EUR 854.4

Mouse GM-CSF ELISA Kit, 96 tests, Quantitative

MGM-CSF-100 1 kit
EUR 578.4

Mouse IL-17F ELISA Kit, 96 tests, Quantitative

MIL-017-140 1 kit
EUR 607.2

Mouse IL-17A ELISA Kit, 96 tests, Quantitative

MIL-17A-185 1 kit
EUR 578.4

Human GM-CSF ELISA Kit, 96 tests, Quantitative

HGM-CSF-100 1 kit
EUR 578.4

Human IL-17A ELISA Kit, 96 tests, Quantitative

HIL-17A-175 1 kit
EUR 578.4

Human IL-17F ELISA Kit, 96 tests, Quantitative

HIL-17F-185 1 kit
EUR 607.2

Corticosterone ELISA Kit, 96 tests, Quantitative

CRS-100 1 kit
EUR 854.4

Alpha-Fetoprotein, AFP ELISA Kit (Quantitative)

DEIA080 96T
EUR 602.4
Description: This AFP ELISA kit is an enzyme linked immunosorbent assay (ELISA) for in vitro quantitative determination of α-fetoprotein (AFP) concentrations in the range of 2-400ng/mL in human serum or plasma samples.

Goat Prolactin ELISA Kit, 96 tests, Quantitative

10735 1 Kit
EUR 927.6

Human Cortisol ELISA Kit, 96 tests, Quantitative

1850 1 kit
EUR 634.8

Monkey Albumin ELISA Kit, 96 tests, Quantitative

7000-10 1 Kit
EUR 927.6

Rat Thiostatin ELISA kit 96 tests, Quantitative

600-730-RTS 1 Kit
EUR 927.6

Human Thrombin ELISA Kit, 96 tests, Quantitative

9625 1 Kit
EUR 927.6

Mouse Ferritin ELISA Kit, 96 tests, Quantitative

9320 1 Kit
EUR 927.6

Mouse Glucagon ELISA Kit, 96 tests, Quantitative

9335 1 Kit
EUR 1074

Rat IL-1 beta ELISA Kit, 96 tests, Quantitative

RIL-01B-100 1 kit
EUR 578.4

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