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Multiplex TaqMan Real-Time PCR Assay for Sensitive Detection of Two Weevil Species (Coleoptera: Curculionidae)

Can quantitative RT-PCR for SARS-CoV-2 help in better management of patients and control of coronavirus disease 2019 pandemic

The emergence of SARS-CoV-2, the causative agent of coronavirus disease 2019 (COVID-19), represents a public health emergency of unprecedented proportion. The global containment efforts have been focused on testing, tracing of contacts and treatment (isolation) of those found COVID-19 positive. Since the whole genome sequences of a number of strains of this novel RNA virus were available in the public domain by early January 2020, a number of real-time polymerase chain reaction (RT-PCR) protocols were designed and used for diagnosis of this infection.
Most RT-PCRs are designed for qualitative COVID-19 reporting (SARS-CoV-2 detected or not detected), but have been used for semi-quantitative estimation of viral load based on cycle threshold value. Our manuscript discusses the utility of quantitative PCR testing for COVID-19 and its patient management benefits.

Multiplex TaqMan Real-Time PCR Assay for Sensitive Detection of Two Weevil Species (Coleoptera: Curculionidae)

Rapid and cost-efficient identification of Naupactus species is becoming a key process for the exportation of citrus fruit from Chile and other countries, considering the quarantine regulations for some species of the cosmopolitan genus Naupactus.
This study deals with the development of a fast and sensitive detection protocol for Naupactus cervinus (Coleoptera: Curculionidae) (Boheman) and Naupactus xanthographus (Coleoptera: Curculionidae) (Germar) based on multiplex TaqMan Real-time polymerase chain reaction. Both N. cervinus and N. xanthographus primer and probe sets achieved species-specific detection in a linear range from 1 pg/μl to 1 × 10-6 pg/μl, allowing detection of as few as 160 copies of template DNA. Non-target amplifications were not detected and a panel composed of 480 test samples had 100% coincidence with the respective morphological identification.

Validation of swab sampling and SYBR Green-based real-time PCR for the diagnosis of Cutaneous Leishmaniasis in French Guiana

 

Recent studies have highlighted the interest of non-invasive sampling procedures coupled to real-time PCR methods for detection of Leishmania species in South America. In French Guiana, sampling method still relied on skin biopsies. Non-invasive protocols should be tested on a large annual cohort to improve routine laboratory diagnosis of Cutaneous Leishmaniasis.
Therefore, we evaluated the performances of a new Leishmania detection and species identification protocol involving cotton swabs and SYBR Green real-time PCR of Hsp70 gene, coupled with Sanger sequencing. Between May 2017 and May 2018, 145 patients with ulcerated lesions compatible with Cutaneous Leishmaniasis were included in the Cayenne Hospital and its remote health centres. Each patient underwent scrapings for smear, skin biopsies for parasite culture and PCR-RFLP (RNA pol II) and cotton swabs for SYBR Green PCR. The most accurate diagnostic test was the SYBR Green PCR on swab sampling, showing a 98% sensitivity.
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SYBR Green qPCR Master Mix (No ROX)

C0006-1g 1g Ask for price
Description: SYBR Green qPCR Master Mix (No ROX)

SYBR Green qPCR Master Mix (No ROX)

C0006-1mg 1mg Ask for price
Description: SYBR Green qPCR Master Mix (No ROX)

SYBR Green qPCR Master Mix (No ROX)

C0006-50mg 50mg Ask for price
Description: SYBR Green qPCR Master Mix (No ROX)

SYBR Green qPCR Master Mix (No ROX)

C0006-5mg 5mg Ask for price
Description: SYBR Green qPCR Master Mix (No ROX)

SYBR Green qPCR Master Mix (No ROX)

HY-K0523 5 mL (500 rxns )
EUR 308.4

SYBR Green qPCR Master Mix (Low ROX)

C0008-10mg 10mg Ask for price
Description: SYBR Green qPCR Master Mix (Low ROX)

SYBR Green qPCR Master Mix (Low ROX)

C0008-1g 1g Ask for price
Description: SYBR Green qPCR Master Mix (Low ROX)

SYBR Green qPCR Master Mix (Low ROX)

C0008-1mg 1mg Ask for price
Description: SYBR Green qPCR Master Mix (Low ROX)

SYBR Green qPCR Master Mix (Low ROX)

C0008-50mg 50mg Ask for price
Description: SYBR Green qPCR Master Mix (Low ROX)

SYBR Green qPCR Master Mix (Low ROX)

C0008-5mg 5mg Ask for price
Description: SYBR Green qPCR Master Mix (Low ROX)

SYBR Green qPCR Master Mix (Low ROX)

HY-K0522 5 mL (500 rxns )
EUR 308.4

SYBR Green qPCR Master Mix (High ROX)

C0007-10mg 10mg Ask for price
Description: SYBR Green qPCR Master Mix (High ROX)

SYBR Green qPCR Master Mix (High ROX)

C0007-1g 1g Ask for price
Description: SYBR Green qPCR Master Mix (High ROX)

SYBR Green qPCR Master Mix (High ROX)

C0007-1mg 1mg Ask for price
Description: SYBR Green qPCR Master Mix (High ROX)

SYBR Green qPCR Master Mix (High ROX)

C0007-50mg 50mg Ask for price
Description: SYBR Green qPCR Master Mix (High ROX)

SYBR Green qPCR Master Mix (High ROX)

C0007-5mg 5mg Ask for price
Description: SYBR Green qPCR Master Mix (High ROX)

SYBR Green qPCR Master Mix (High ROX)

HY-K0521 1 mL (100 rxns)
EUR 135.6

2x SYBR Green qPCR Master Mix (Low ROX)

B21702 5 ml
EUR 268.8
Description: 2x SYBR Green qPCR master mix (Low ROX) utilizes a special performance-enhanced Taq DNA polymerase protected via a hot-start activation technique, and optimized qPCR buffer system to perform SYBR

2x SYBR Green qPCR Master Mix (Low ROX)

B21703 25 ml
EUR 1027.2
Description: 2x SYBR Green qPCR master mix (Low ROX) utilizes a special performance-enhanced Taq DNA polymerase protected via a hot-start activation technique, and optimized qPCR buffer system to perform SYBR

2x SYBR Green qPCR Master Mix (High ROX)

B21402 5 ml
EUR 268.8
Description: 2x SYBR Green qPCR master mix (High ROX) utilizes a special performance-enhanced Taq DNA polymerase protected via a hot-start activation technique, and optimized qPCR buffer system to perform SYBR

2x SYBR Green qPCR Master Mix (High ROX)

B21403 25 ml
EUR 1027.2
Description: 2x SYBR Green qPCR master mix (High ROX) utilizes a special performance-enhanced Taq DNA polymerase protected via a hot-start activation technique, and optimized qPCR buffer system to perform SYBR

AceQ qPCR SYBR® Green Master Mix

Q111-02 500 rxn (20 μl/rxn)
EUR 265.2

AceQ qPCR SYBR® Green Master Mix

Q111-03 2500 rxn (20 μl/rxn)
EUR 775.2

HotStart™ 2X SYBR Green qPCR Master Mix

K1070-25 25ml
EUR 336

HotStart™ 2X SYBR Green qPCR Master Mix

K1070-5 5ml
EUR 96

HotStart™ 2X SYBR Green qPCR Master Mix

K1070-50 50ml
EUR 672

ig SYBR Green qPCR 2X Master Mix - 200 Reactions

3354 200
EUR 110
Description: Intact Genomics SYBR Green qPCR 2X Master Mix master mix is a ready-to-use cocktail containing all components except primers and template, for the amplification and detection of DNA in qPCR. The Ig SYBR® Green qPCR 2x master mix with integrated chemically-modified hot start Taq DNA polymerase, SYBR® Green I fluorescent dye, ROX dye, MgCl2, dNTPs and stabilizers. This master mix is ideal for high-throughput real-time PCR screening and validation. The amplification step features a high quality hot start Taq DNA Polymerase which offers higher fidelity and better amplification.

HotStart™ Universal 2X SYBR Green qPCR Master Mix

K1170-25 25ml
EUR 336
Description: qPCR Kit, Universal SYBR Green I Master Mix (Rox)

HotStart™ Universal 2X SYBR Green qPCR Master Mix

K1170-5 5ml
EUR 96
Description: qPCR Kit, Universal SYBR Green I Master Mix (Rox)

HotStart™ Universal 2X SYBR Green qPCR Master Mix

K1170-50 50ml
EUR 672
Description: qPCR Kit, Universal SYBR Green I Master Mix (Rox)

Maxima SYBR Green/ROX qPCR Master Mix - 200 x 25 µL rxns

THK0221 each Ask for price

Maxima SYBR Green/ROX qPCR Master Mix - 1,000 x 25 µL rxns

THK0222 each Ask for price

Maxima SYBR Green/ROX qPCR Master Mix - 4,000 x 25 µL rxns

THK0223 each
EUR 3324.5

Maxima SYBR Green/Fluorescein qPCR Master Mix - 200 x 25 µL rxns

THK0241 each Ask for price

Maxima SYBR Green/Fluorescein qPCR Master Mix - 1,000 x 25 µL rxns

THK0242 each Ask for price

Maxima SYBR Green/Fluorescein qPCR Master Mix - 4,000 x 25 µL rxns

THK0243 each Ask for price

Maxima SYBR Green qPCR Master Mix, ROX Solution provided - 200 x 25 µL rxns

THK0251 each
EUR 232.17

Maxima SYBR Green qPCR Master Mix, ROX Solution provided - 1,000 x 25 µL rxns

THK0252 each
EUR 1046.4

Maxima SYBR Green qPCR Master Mix, ROX Solution provided - 4,000 x 25 µL rxns

THK0253 each Ask for price

BioEasy Master Mix (SYBR Green)

TRI-B25L1 200T
EUR 403.2

ChamQ SYBR qPCR Master Mix

Q311-02 500 rxns (20 μl/rxn)
EUR 120.55

ChamQ SYBR qPCR Master Mix

Q311-03 2,500 rxns (20 μl/rxn)
EUR 543.5

ChamQ SYBR qPCR Master Mix

Q311-00-100rxns 100 rxns
EUR 1.09

ChamQ SYBR qPCR Master Mix

Q311-02-500rxns 500 rxns
EUR 124.83

ChamQ SYBR qPCR Master Mix

Q311-03-2500rxns 2,500 rxns
EUR 562.79

Apex qPCR GREEN Master Mix

42-116PG 400 x 25µl Reactions/Unit
EUR 341.44
Description: 2X, Without ROX, 5ml

Apex qPCR GREEN Master Mix

42-117PG 4000 x 25µl Reactions/Unit
EUR 2926.74
Description: 2X, Without ROX, 50ml

Apex qPCR GREEN Master Mix

42-118PG 400 x 25µl Reactions/Unit
EUR 338.88
Description: 2X, Low ROX, 5ml

Apex qPCR GREEN Master Mix

42-119PG 4000 x 25µl Reactions/Unit
EUR 2926.74
Description: 2X, Low ROX, 50ml

Apex qPCR GREEN Master Mix

42-120PG 400 x 25µl Reactions/Unit
EUR 341.44
Description: 2X, High ROX, 5ml

Apex qPCR GREEN Master Mix

42-121PG 4000 x 25µl Reactions/Unit
EUR 3095.57
Description: 2X, High ROX, 50ml

BioEasy Master Mix (SYBR Green, No ROX)

TRI-B25L1D 200T
EUR 403.2

BioEasy Master Mix (SYBR Green, low ROX)

TRI-B25L1C 200T
EUR 403.2

BioEasy Master Mix (SYBR Green, high ROX)

TRI-B25L1B 200T
EUR 403.2

ChamQ SYBR Color qPCR Master Mix

Q411-02 500 rxns (20 μl/rxn)
EUR 140.63

ChamQ SYBR Color qPCR Master Mix

Q411-03 2,500 rxns (20 μl/rxn)
EUR 634

ChamQ SYBR Color qPCR Master Mix

Q411-00-100rxns 100 rxns
EUR 1.09

ChamQ SYBR Color qPCR Master Mix

Q411-02-500rxns 500 rxns
EUR 145.62

ChamQ SYBR Color qPCR Master Mix

Q411-03-2500rxns 2,500 rxns
EUR 656.51

AceQ Universal SYBR qPCR Master Mix

Q511-02 500 rxn (20 μl/rxn)
EUR 152.72

AceQ Universal SYBR qPCR Master Mix

Q511-03 2500 rxn (20 μl/rxn)
EUR 688.5

AceQ Universal SYBR qPCR Master Mix

Q511-00-100rxns 100 rxns
EUR 1.09

AceQ Universal SYBR qPCR Master Mix

Q511-02-500rxns 500 rxns
EUR 158.14

AceQ Universal SYBR qPCR Master Mix

Q511-03-2500rxns 2,500 rxns
EUR 712.94

miRNA Universal SYBR qPCR Master Mix

MQ101-01 125 rxn(20 μl/rxn)
EUR 38.9

miRNA Universal SYBR qPCR Master Mix

MQ101-02 500 rxn(20 μl/rxn)
EUR 145

ChamQ Universal SYBR qPCR Master Mix

Q711-02 500 rxn (20 μl/rxn)
EUR 152.72

ChamQ Universal SYBR qPCR Master Mix

Q711-03 2500 rxn (20 μl/rxn)
EUR 688.5

miRNA Universal SYBR qPCR Master Mix

MQ101-00-100rxns 100 rxns
EUR 1.09

miRNA Universal SYBR qPCR Master Mix

MQ101-01-125rxns 125 rxns
EUR 40.28

miRNA Universal SYBR qPCR Master Mix

MQ101-02-500rxns 500 rxns
EUR 150.15

ChamQ Universal SYBR qPCR Master Mix

Q711-00-100rxns 100 rxns
EUR 1.09

ChamQ Universal SYBR qPCR Master Mix

Q711-02-500rxns 500 rxns
EUR 158.14

ChamQ Universal SYBR qPCR Master Mix

Q711-03-2500rxns 2,500 rxns
EUR 712.94

2×SYBR Green qPCR Mix

MBS8579840-200Tests 200Tests
EUR 285

2×SYBR Green qPCR Mix

MBS8579840-50Tests 50Tests
EUR 180

2×SYBR Green qPCR Mix

MBS8579840-5x200Tests 5x200Tests
EUR 1230

2×SYBR Green qPCR Mix

MBS8579840-5x50Tests 5x50Tests
EUR 770

ChamQ SYBR qPCR Master Mix (Without ROX)

Q321-02 500 rxns (20 μl/rxn)
EUR 120.55

ChamQ SYBR qPCR Master Mix (Without ROX)

Q321-03 2,500 rxns (20 μl/rxn)
EUR 543.5

Taq Pro Universal SYBR qPCR Master Mix

Q712-02 500 rxns (20 μl/rxn)
EUR 161

Taq Pro Universal SYBR qPCR Master Mix

Q712-03 2,500 rxns (20 μl/rxn)
EUR 725

ChamQ SYBR qPCR Master Mix (Without ROX)

Q321-02-500rxns 500 rxns
EUR 124.83

ChamQ SYBR qPCR Master Mix (Without ROX)

Q321-03-2500rxns 2,500 rxns
EUR 562.79

Taq Pro Universal SYBR qPCR Master Mix

Q712-00-100rxns 100 rxns
EUR 1.09

Taq Pro Universal SYBR qPCR Master Mix

Q712-02-500rxns 500 rxns
EUR 166.72

Taq Pro Universal SYBR qPCR Master Mix

Q712-03-2500rxns 2,500 rxns
EUR 750.74

ViPrimePLUSHigh 5X One Step RT-qPCR Green Master Mix I (SYBR® Green Dye), 100rxns

QLMM10 each Ask for price

HotStart™ 2X FAST Green qPCR Master Mix

K1171-25 25ml
EUR 424
Description: Reverse Transcription & PCR|qPCR/Real-time PCR

HotStart™ 2X FAST Green qPCR Master Mix

K1171-5 5ml
EUR 132
Description: Reverse Transcription & PCR|qPCR/Real-time PCR

HotStart™ 2X FAST Green qPCR Master Mix

K1171-50 50ml
EUR 826.4
Description: Reverse Transcription & PCR|qPCR/Real-time PCR

ChamQ SYBR qPCR Master Mix (Low ROX Premixed)

Q331-02 500 rxns (20 μl/rxn)
EUR 120.55

ChamQ SYBR qPCR Master Mix (Low ROX Premixed)

Q331-03 2,500 rxns (20 μl/rxn)
EUR 543.5

ChamQ SYBR qPCR Master Mix (Low ROX Premixed)

Q331-02-500rxns 500 rxns
EUR 124.83

ChamQ SYBR qPCR Master Mix (Low ROX Premixed)

Q331-03-2500rxns 2,500 rxns
EUR 562.79

ChamQ SYBR qPCR Master Mix (High ROX Premixed)

Q341-02 500 rxns (20 μl/rxn)
EUR 120.55

ChamQ SYBR qPCR Master Mix (High ROX Premixed)

Q341-03 2,500 rxns (20 μl/rxn)
EUR 543.5

ChamQ SYBR Color qPCR Master Mix (Without ROX)

Q421-02 500 rxns (20 μl/rxn)
EUR 140.63

ChamQ SYBR Color qPCR Master Mix (Without ROX)

Q421-03 2,500 rxns (20 μl/rxn)
EUR 634

ChamQ SYBR qPCR Master Mix (High ROX Premixed)

Q341-02-500rxns 500 rxns
EUR 124.83

ChamQ SYBR qPCR Master Mix (High ROX Premixed)

Q341-03-2500rxns 2,500 rxns
EUR 562.79

ChamQ SYBR Color qPCR Master Mix (Without ROX)

Q421-02-500rxns 500 rxns
EUR 145.62

ChamQ SYBR Color qPCR Master Mix (Without ROX)

Q421-03-2500rxns 2,500 rxns
EUR 656.51

HotStart™ Universal 2X FAST Green qPCR Master Mix

K1172-25 25ml
EUR 424
Description: Reverse Transcription & PCR|qPCR/Real-time PCR

HotStart™ Universal 2X FAST Green qPCR Master Mix

K1172-5 5ml
EUR 132
Description: Reverse Transcription & PCR|qPCR/Real-time PCR

HotStart™ Universal 2X FAST Green qPCR Master Mix

K1172-50 50ml
EUR 826.4
Description: Reverse Transcription & PCR|qPCR/Real-time PCR

ViPrimePLUS Taq qPCR Green Master Mix I, 150rxns

QLMM12 each Ask for price

ViPrimePLUS Taq qPCR Green Master Mix II, 150rxns

QLMM17 each Ask for price

ViPrimePLUS AtTaq qPCR Green Master Mix I, 150rxns

QLMM02 each Ask for price

ViPrimePLUS AtTaq qPCR Green Master Mix II, 150rxns

QLMM07 each Ask for price

ChamQ SYBR Color qPCR Master Mix (Low ROX Premixed)

Q431-02 500 rxns (20 μl/rxn)
EUR 140.63

ChamQ SYBR Color qPCR Master Mix (Low ROX Premixed)

Q431-03 2,500 rxns (20 μl/rxn)
EUR 634

ChamQ SYBR Color qPCR Master Mix (Low ROX Premixed)

Q431-02-500rxns 500 rxns
EUR 145.62

ChamQ SYBR Color qPCR Master Mix (Low ROX Premixed)

Q431-03-2500rxns 2,500 rxns
EUR 656.51

ChamQ SYBR Color qPCR Master Mix (High ROX Premixed)

Q441-02 500 rxns (20 μl/rxn)
EUR 140.63

ChamQ SYBR Color qPCR Master Mix (High ROX Premixed)

Q441-03 2,500 rxns (20 μl/rxn)
EUR 634

ChamQ SYBR Color qPCR Master Mix (High ROX Premixed)

Q441-02-500rxns 500 rxns
EUR 145.62

ChamQ SYBR Color qPCR Master Mix (High ROX Premixed)

Q441-03-2500rxns 2,500 rxns
EUR 656.51

TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green *No ROX*

17270 1 mL
EUR 203
Description: TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR.

TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green *No ROX*

17270-1mL 1 mL
EUR 99
Description: TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR.
Mean PCR cycle threshold (Ct) was of 24.4 Ct (min=17 Ct, max=36 Ct) and was inferior to 35 Ct in 97.6% of samples. All samples positive with real-time PCR SYBR Green were successfully identified at the species level by DNA sequencing. This new method should be considered for routine diagnosis of Cutaneous Leishmaniasis in South America and especially for remote areas, as non-invasive collection tools are easier to use and require less precautions for transportation.

Development and clinical validation of a pangenotypic PCR-based assay for the detection and quantification of hepatitis E virus (Orthopevirus A genus)

 

The objective of this study was to design a pangenotypic PCR-based assay for the detection and quantification of hepatitis E virus (HEV) RNA from across the entire spectrum of described genotypes belonging to the Orthohepevirus A genus. The optimal conditions and the performance of the assay were determined by testing the WHO standard strain (6219/10) and the WHO HEV panel (8578/13).
Similarly, performance comparisons were made with two commercial assays (Real Star HEV RT-PCR 2.0 and ampliCube HEV 2.0 Quant) to detect HEV RNA at concentrations below 1,000 IU/mL with viral strains from the WHO and to test samples from 54 patients with acute hepatitis. The assay presented in this study was able to detect the entire spectrum of described genotypes belonging to the Orthohepevirus A genus, demonstrating a better performance than both commercial kits. This procedure may represent a significant improvement in the molecular diagnosis of HEV infection.

SARS-CoV-2 and RT-PCR in asymptomatic patients: Results of a cohort of workers at El Dorado International Airport in Bogotá, 2020

 

Introduction: The 2019 coronavirus pandemic (COVID-19) has caused around 25 million cases worldwide. Asymptomatic patients have been described as potential sources of transmission. However, there are difficulties to detect them and to establish their role in the dynamics of virus transmission, which hinders the implementation of prevention strategies. Objective: To describe the behavior of asymptomatic SARS-CoV-2 virus infection in a cohort of workers at the El Dorado “Luis Carlos Galán Sarmiento” International Airport in Bogotá, Colombia. Materials and methods: A prospective cohort of 212 workers from the El Dorado airport was designed. The follow-up began in June, 2020. A survey was used to characterize health and work conditions.
Every 21 day, a nasopharyngeal swab was taken to identify the presence of SARS-CoV-2 using RT-PCR. We analyzed the behavior of the cycle threshold (ORF1ab and N genes) according to the day of follow-up. Results: In the first three follow-ups of the cohort, we found an incidence of SARS-CoV-2 infection of 16.51%. The proportion of positive contacts was 14.08%. The median threshold for cycle threshold was 33.53. Conclusion: We characterized the asymptomatic SARS-CoV-2 infection in a cohort of workers. The identification of asymptomatic infected persons continues to be a challenge for epidemiological surveillance systems.
Introducción. La pandemia de COVID-19 ha ocasionado cerca de 25 millones de casos en el mundo. Se ha descrito que los pacientes asintomáticos pueden ser fuentes de transmisión. Sin embargo, es difícil detectarlos y no es claro su papel en la dinámica de transmisión del virus, lo que obstaculiza la implementación de estrategias para la prevención.
Objetivo. Describir el comportamiento de la infección asintomática por SARS-CoV-2 en una cohorte de trabajadores del Aeropuerto Internacional El Dorado “Luis Carlos Galán Sarmiento” de Bogotá, Colombia. Materiales y métodos. Se diseñó una cohorte prospectiva de trabajadores del Aeropuerto El Dorado. El seguimiento se inició en junio de 2020 con una encuesta a cada trabajador para caracterizar sus condiciones de salud y trabajo. Cada 21 días se tomó una muestra de hisopado nasofaríngeo para detectar la presencia del SARS-CoV-2 mediante reacción en cadena de la polimerasa con transcriptasa inversa (RT-PCR). Se analizó el comportamiento del umbral del ciclo (cycle threshold) de los genes ORF1ab y N según el día de seguimiento.
Resultados. En los primeros tres seguimientos de la cohorte se encontró una incidencia de la infección por SARS-CoV-2 del 16,51 %. La proporción de contactos positivos fue del 14,08 %. La mediana del umbral del ciclo fue de 33,53. Conclusión. Se determinaron las características de la infección asintomática por el SARSCoV-2 en una cohorte de trabajadores. La detección de infectados asintomáticos sigue siendo un reto para los sistemas de vigilancia epidemiológica.

HBV Real-TM Quant Real Time PCR Test for quantitative detection of HBV (25 μl Reaction Mix)

V5-100-2FRT 100
EUR 1362.5

HDV Real-TM Quant Real Time PCR Test for quantitative detection of HDV

V3-100-2FRT 100
EUR 1358.14

CMV Real-TM Quant Real Time PCR Test for quantitative detection of CMV

V7-100-2FRT 100
EUR 898.16

EBV Real-TM Quant Real Time PCR test for quantitative detection of EBV

V9-100FRT 100
EUR 876.36

EBV Real-TM Quant Real Time PCR test for quantitative detection of EBV

V9-50FRT 50
EUR 560.26

HHV6 Real-TM Quant Real Time PCR test for quantitative detection of HHV6

V10-100FRT 100
EUR 651.82

HHV7 Real-TM Quant Real Time PCR test for quantitative detection of HHV7

V17-100FRT 100
EUR 1338.52

HBV Real-TM Quant Dx Real Time PCR Test for quantitative detection of HBV (96 ready to use lyophilized tubes - 50 μl Reaction Mix)

V5-96-3FRT 96
EUR 1100.9

Lysteria monocytogenes Real-TM Quant Real Time PCR kit for quantitative detection L.monocytogenes

B14-50FRT 50
EUR 457.8

Green Dye One Step qRT-PCR Master Mix For Quantitative Real Time PCR With ROX Dye

MB1004 100 reactions
EUR 239
Description:

Boster's Green Dye One Step qRT-PCR Master Mix contains all the reagents necessary for reverse transcription and PCR amplification to occur in a single PCR re-action tube, without the template. The Master Mix contains a qRT-PCR Enzyme Mix and a Green Dye qPCR MasterMix, including proprietary Reverse Transcriptase, Ribonuclease Inhibitor, dNTPs and a finely balanced ratio of Oligo (dT)s and Random Primers. The Master Mix also has the high specificity of hot start polymerase. This Master Mix offers the user an efficient and reliable alternative to conventional “two-step” qRT-PCR. Gene-specific primers must be used along with this kit.

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More about our proprietary Reverse Transcriptase:

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The native Reverse Transcriptase has RNase H capacity and degrades mRNA. Using a series of strategic targeted mutations, our scientists successfully nullified the RNase H activity of our RT enzyme, thus preventing RNA degradation during first-strand cDNA synthesis, resulting in higher yields and increase in the achievable length of synthesized cDNA. The engineered Reverse Transcriptase also contains a fidelity‐enhancing sub-unit which ensures superior accuracy in reverse transcription.

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Green Dye One Step qRT-PCR Master Mix For Quantitative Real Time PCR With ROX Dye

MB1004-100Reactions 100 Reactions
EUR 392.4

Pseudomonas aeruginosa Quant Real-TM Real Time PCR kit for quantitative detection of Pseudomonas aeruginosa

B76-50FRT 50
EUR 494.86

Streptococcus pyogenes Real-TM Quant Real Time PCR kit for quantitative detection of Streptococcus pyogenes

B82-100FRT 100
EUR 651.82

HPV 16/18 Real-TM Quant Real Time PCR test for quantitative detection of HPV 16 and 18

V12-100FRT 100
EUR 763

HCV Real-TM Quant Real Time PCR Test for quantitative detection of HCV (25 μl Reaction Mix)

V1-100-2FRT 100
EUR 784.8

Streptococcus B Real-TM Quant Real Time PCR test for quantitative detection of Streptococcus B

B77-100FRT 100
EUR 651.82

JCV/BKV Virus Real-TM Quant Real Time PCR test for quantitative detection of JC Virus and BK virus

V71-100FRT 100
EUR 1079.1

Legionella pneumophila Real-TM Real Time PCR test for quantitative detection of Legionella pneumophila

B50-50FRT 50
EUR 538.46

CMV-EBV-HHV6 Quant Real-TM Multiplex Real Time PCR test for quantitative detection of CMV, EBV, HHV6

V48-100FRT 100
EUR 1233.88

Candidosis Real-TM-Real Time PCR kit for quantitative detection of C.albicans, C.glabrata, C.krusei,C.parapsilosis, C. tropicansi

F5-100FRT 100
EUR 1257.86

Gardnerella vaginalis/Lactobac. species Real-TM Quant Real Time PCR test for quantitative detection of G.vaginalis and Lactobacillus

R-B7-100FRT 100
EUR 741.2

HIV Real-TM Quant Dx Real Time PCR Test for quantitative detection of HIV 96 ready to use lyophilized tubes - 50 μl Reaction Mix

V0-96-3FRT 96
EUR 1549.98

HCV Real-TM Quant Dx Real Time PCR Test for quantitative detection of HCV 96 ready to use lyophilized tubes - 50 μl Reaction Mix

V1-96-3FRT 96
EUR 1414.82

HBV PCR Fluorescence Quantitative Detection Kit

TRI-B01M1B 48T
EUR 403.2

HBV PCR Fluorescence Quantitative Detection Kit

TRI-B01S1B 32T
EUR 277.2

HPV genotypes 14 Real-TM Quant Real Time PCR test for quantitative detection and genotyping of HPV (16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, 59, 66 and 68)

V67-100FRT 100
EUR 1571.78

HPV genotypes 21 Real-TM Quant Real Time PCR test for quantitative detection and genotyping of HPV (6, 11, 16, 18, 26, 31, 33, 35, 39, 44, 45, 51, 52, 53, 56, 58, 59, 66, 68, 73, 82)

V21-100FRT 96
EUR 2594.2

HPV 14 Screen & 16,18,45 Typing Real-TM Quant Real Time PCR test for quantitative detection of 14 HPV (16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, 59, 66 and 68) and genotyping of HPV 16, 18, 45

V31-100-F-FRT 100
EUR 1325.44

HBV PCR Fluorescence Quantitative Diagnostic Kit(with internal control)

TRI-B01M1G 48T
EUR 700.56

EpiQuik Quantitative PCR Fast Kit 

P-1029
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  • 200 Reactions
  • 100 Reactions
  • 200 Reactions

Human HBV preS1Ab (HBV preS1Ab) quantitative detection ELISA Kit

MBS3809945-10x96StripWells 10x96-Strip-Wells
EUR 6545

Human HBV preS1Ab (HBV preS1Ab) quantitative detection ELISA Kit

MBS3809945-5x96StripWells 5x96-Strip-Wells
EUR 3325

Human HBV preS1Ab (HBV preS1Ab) quantitative detection ELISA Kit

MBS3809945-96StripWells 96-Strip-Wells
EUR 720

Human HBV preS2Ab (HBV preS2Ab) quantitative detection ELISA Kit

MBS3809946-10x96StripWells 10x96-Strip-Wells
EUR 6545

Human HBV preS2Ab (HBV preS2Ab) quantitative detection ELISA Kit

MBS3809946-5x96StripWells 5x96-Strip-Wells
EUR 3325

Human HBV preS2Ab (HBV preS2Ab) quantitative detection ELISA Kit

MBS3809946-96StripWells 96-Strip-Wells
EUR 720

HCV PCR Fluorescence Quantitative Detection Kit

TRI-B02M1B 48T
EUR 932.4

HCV PCR Fluorescence Quantitative Detection Kit

TRI-B02S1B 32T
EUR 630

Rat Real-time PCR DNA Quantitation after QPCR DNA Damage Analysis Kit

DD2R 1 Kit
EUR 550

Fluorescent quantitative PCR calibration dye

TRI-A41S1 96T
EUR 370.44

TRUEscript RT MasterMix (for real time PCR)(First strand reverse transcription premixed mix/fluorescence quantitation)

MBS355987-100Tests 100Tests
EUR 410

TRUEscript RT MasterMix (for real time PCR)(First strand reverse transcription premixed mix/fluorescence quantitation)

MBS355987-50Tests 50Tests
EUR 300

TRUEscript RT MasterMix (for real time PCR)(First strand reverse transcription premixed mix/fluorescence quantitation)

MBS355987-5x100Tests 5x100Tests
EUR 1550

Human Real-time PCR DNA Quantitation after QPCR DNA Damage Analysis Kit

DD2H 1 Kit
EUR 550

Mouse Real-time PCR DNA Quantitation after QPCR DNA Damage Analysis Kit

DD2M 1 Kit
EUR 550

qPCR ProbesMasterMaster mix for quantitative real-time PCR using labeled DNA probes, 2 x conc. master mix** shipped ice packs - must be shipped via overnight service

M-PCR-360L 10 x 1,25 ml
EUR 818
Description: qPCR ProbesMasterMaster mix for quantitative real-time PCR using labeled DNA probes, 2 x conc. master mix** shipped ice packs - must be shipped via overnight service

qPCR ProbesMasterMaster mix for quantitative real-time PCR using labeled DNA probes, 2 x conc. master mix** shipped ice packs - must be shipped via overnight service

M-PCR-360S 2 x 1,25 ml
EUR 205
Description: qPCR ProbesMasterMaster mix for quantitative real-time PCR using labeled DNA probes, 2 x conc. master mix** shipped ice packs - must be shipped via overnight service

qPCR ProbesMaster UNGMaster mix for quantitative real-time PCR using labeled DNA probes, 2 x conc. master mix** shipped ice packs - must be shipped via overnight service

M-PCR-363L 10 x 1,25 ml
EUR 982
Description: qPCR ProbesMaster UNGMaster mix for quantitative real-time PCR using labeled DNA probes, 2 x conc. master mix** shipped ice packs - must be shipped via overnight service

qPCR ProbesMaster UNGMaster mix for quantitative real-time PCR using labeled DNA probes, 2 x conc. master mix** shipped ice packs - must be shipped via overnight service

M-PCR-363S 2 x 1,25 ml
EUR 246
Description: qPCR ProbesMaster UNGMaster mix for quantitative real-time PCR using labeled DNA probes, 2 x conc. master mix** shipped ice packs - must be shipped via overnight service

qPCR ProbesMaster highROXMaster mix for quantitative real-time PCR using labeled DNA probes, 2x conc. master mix** shipped ice packs - must be shipped via overnight service

M-PCR-362L 10 x 1,25 ml
EUR 818
Description: qPCR ProbesMaster highROXMaster mix for quantitative real-time PCR using labeled DNA probes, 2x conc. master mix** shipped ice packs - must be shipped via overnight service

qPCR ProbesMaster highROXMaster mix for quantitative real-time PCR using labeled DNA probes, 2x conc. master mix** shipped ice packs - must be shipped via overnight service

M-PCR-362S 2 x 1,25 ml
EUR 205
Description: qPCR ProbesMaster highROXMaster mix for quantitative real-time PCR using labeled DNA probes, 2x conc. master mix** shipped ice packs - must be shipped via overnight service

qPCR ProbesMaster lowROXMaster mix for quantitative real-time PCR using labeled DNA probes, 2 x conc. master mix** shipped ice packs - must be shipped via overnight service

M-PCR-361L 10 x 1,25 ml
EUR 818
Description: qPCR ProbesMaster lowROXMaster mix for quantitative real-time PCR using labeled DNA probes, 2 x conc. master mix** shipped ice packs - must be shipped via overnight service

qPCR ProbesMaster lowROXMaster mix for quantitative real-time PCR using labeled DNA probes, 2 x conc. master mix** shipped ice packs - must be shipped via overnight service

M-PCR-361S 2 x 1,25 ml
EUR 205
Description: qPCR ProbesMaster lowROXMaster mix for quantitative real-time PCR using labeled DNA probes, 2 x conc. master mix** shipped ice packs - must be shipped via overnight service

Staphylococcus aureus PCR Fluorescence Quantitative Detection kit

TRI-B39M1 48T
EUR 498.96

Staphylococcus aureus PCR Fluorescence Quantitative Detection kit

TRI-B39S1 24T
EUR 289.8

qPCR ProbesMaster UNG lowROXMaster mix for quantitative real-time PCR using labeled DNA probes, 2 x conc. master mix** shipped ice packs - must be shipped via overnight service

M-PCR-364L 10 x 1,25 ml
EUR 982
Description: qPCR ProbesMaster UNG lowROXMaster mix for quantitative real-time PCR using labeled DNA probes, 2 x conc. master mix** shipped ice packs - must be shipped via overnight service

qPCR ProbesMaster UNG lowROXMaster mix for quantitative real-time PCR using labeled DNA probes, 2 x conc. master mix** shipped ice packs - must be shipped via overnight service

M-PCR-364S 2 x 1,25 ml
EUR 246
Description: qPCR ProbesMaster UNG lowROXMaster mix for quantitative real-time PCR using labeled DNA probes, 2 x conc. master mix** shipped ice packs - must be shipped via overnight service

qPCR ProbesMaster UNG highROXMaster mix for quantitative real-time PCR using labeled DNA probes, 2 x conc. master mix** shipped ice packs - must be shipped via overnight service

M-PCR-365L 10 x 1,25 ml
EUR 982
Description: qPCR ProbesMaster UNG highROXMaster mix for quantitative real-time PCR using labeled DNA probes, 2 x conc. master mix** shipped ice packs - must be shipped via overnight service

qPCR ProbesMaster UNG highROXMaster mix for quantitative real-time PCR using labeled DNA probes, 2 x conc. master mix** shipped ice packs - must be shipped via overnight service

M-PCR-365S 2 x 1,25 ml
EUR 246
Description: qPCR ProbesMaster UNG highROXMaster mix for quantitative real-time PCR using labeled DNA probes, 2 x conc. master mix** shipped ice packs - must be shipped via overnight service

Pseudomonas aeruginosa PCR Fluorescence Quantitative Detection kit

TRI-B41M1 48T
EUR 498.96

Pseudomonas aeruginosa PCR Fluorescence Quantitative Detection kit

TRI-B41S1 24T
EUR 289.8

Listeria monocytogenes PCR Fluorescence Quantitative Detection kit

TRI-B54M1 48T
EUR 498.96

Listeria monocytogenes PCR Fluorescence Quantitative Detection kit

TRI-B54S1 24T
EUR 289.8

Escherichia coli O157 PCR Fluorescence Quantitative Detection kit

TRI-B38M1 48T
EUR 498.96

Escherichia coli O157 PCR Fluorescence Quantitative Detection kit

TRI-B38S1 24T
EUR 289.8

Vibrio Parahemo/yticus PCR Fluorescence Quantitative Detection kit

TRI-B55M1 48T
EUR 498.96

Vibrio Parahemo/yticus PCR Fluorescence Quantitative Detection kit

TRI-B55S1 24T
EUR 289.8

HIV RT-PCR Fluorescence Quantitative Detection Kit

TRI-B24M1C 48T
EUR 1630.44

HIV RT-PCR Fluorescence Quantitative Detection Kit

TRI-B24S1C 32T
EUR 1086.12

MRSA Quant Real-TM Real Time PCR kit

B78-100FRT 100
EUR 784.8

Benedict’s Quantitative Reagent

R003-500ML 1 unit
EUR 7.09
Description: Benedict’s Quantitative Reagent

Mycobacterium tuberculoisis (TB) PCR Fluorescence Quantitative Diagnostic Kit

TRI-B19M1 48T
EUR 680.4

Mycobacterium tuberculoisis (TB) PCR Fluorescence Quantitative Diagnostic Kit

TRI-B19S1 32T
EUR 478.8

RT-PCR quantitative Covid-19 assay CE-IVD

CLO-RT-25 48 reactions
EUR 2513
Description: RT-PCR quantitative Covid-19 assay CE-IVD

Tiamulin ELISA Kit, Quantitative

DEIA036 96T
EUR 1414.8
Description: This kit is intended for quantitative analysis of tiamulin residue in animal tissue (pork and chicken, etc).

Fumonisin Quantitative Test Kit

Q603 96T
EUR 200
Description: Feed Test

Benedict`s Reagent Quantitative

82949 500 ml
EUR 2.6
Description: Part A

T2 toxin Quantitative Test Kit

Q604 96T
EUR 200
Description: Feed Test

Zearalenone Quantitative Test Kit

Q601 96T
EUR 200
Description: Feed Test

Human RBP4 Quantitative ELISA Kit

LF-EK70030 1×96T
EUR 722.4

Aflatoxin B1 Quantitative Test Kit

Q401 96T
EUR 200
Description: Feed Test

Ochratoxin A Quantitative Test Kit

Q605 96T
EUR 200
Description: Feed Test

Biotin quantitative Determination Kit

MBS2563934-5x96Tests 5x96Tests
EUR 1360

Biotin quantitative Determination Kit

MBS2563934-96Tests 96Tests
EUR 305

Total protein quantitative assay kit

BC032-100tube96samples 100tube/96samples
EUR 110

Total protein quantitative Assay Kit

SH0172 100T/96Sample
EUR 160

Total protein quantitative Assay Kit

SH0309-1 50T/48Sample
EUR 150

Total protein quantitative Assay Kit

SH0309-2 100T/96Sample
EUR 160

DNA Oxidative Damage Kit - Quantitative

DN-002 20 tests
EUR 656

HCV/HBV/HIV1/HIV2 Real-TM Real Time PCR kit

V62-100FRT 100
EUR 1560.88

Mouse NoroVirus ELISA Kit(Quantitative )

SL0661Mo -
EUR 528

TRUEscript RT kit (With gDNA Eraser)(With genome removal, for fluorescence quantitative PCR)

MBS355988-100Tests 100Tests
EUR 465

TRUEscript RT kit (With gDNA Eraser)(With genome removal, for fluorescence quantitative PCR)

MBS355988-50Tests 50Tests
EUR 355

TRUEscript RT kit (With gDNA Eraser)(With genome removal, for fluorescence quantitative PCR)

MBS355988-5x100Tests 5x100Tests
EUR 2095

Mouse Mycoplasma Elisa kit(quantitative)

EK730424 96 Wells
EUR 0.68

Human Mumps IgG ELISA Kit (quantitative)

SL3197Hu 96 Tests
EUR 468

TBE / FSME IgG quantitative

GWB-AC53EA 1x96 Assays Ask for price

Rat C3 ELISA Kit, 96 tests, Quantitative

6230 1 kit
EUR 854.4

HCV RT-PCR Fluorescence Quantitative Diagnostic Kit(with internal control)

TRI-B02M1F 48T
EUR 1310.4

THERMOscript RT kit (With gDNA Eraser)(With genome removal, for fluorescence quantitative PCR)

MBS355990-100Tests 100Tests
EUR 565

THERMOscript RT kit (With gDNA Eraser)(With genome removal, for fluorescence quantitative PCR)

MBS355990-50Tests 50Tests
EUR 400

THERMOscript RT kit (With gDNA Eraser)(With genome removal, for fluorescence quantitative PCR)

MBS355990-5x100Tests 5x100Tests
EUR 2545

Lactate ELISA Kit, 96 tests, Quantitative

10795 1 Kit
EUR 927.6

Pig IgM ELISA Kit, 96 tests, Quantitative

9080 1 kit
EUR 854.4

Rat IgA ELISA kit 96 tests, Quantitative

6410-10 1 kit
EUR 607.2

Rat IgG ELISA Kit, 96 tests, Quantitative

6420 1 kit
EUR 781.2

Rat IgE ELISA Kit, 96 tests, Quantitative

6470 1 kit
EUR 927.6

Rat IgM ELISA Kit, 96 tests, Quantitative

6480 1 Kit
EUR 854.4

Cat IgG ELISA Kit, 96 tests, Quantitative

300-100-CGG 1 kit
EUR 854.4

Cat IgM ELISA kit 96 tests, Quantitative

300-170-CGM 1 kit
EUR 854.4

Cat IgA ELISA Kit, 96 tests, Quantitative

300-180-CGA 1 kit
EUR 854.4

Cat IgE ELISA Kit, 96 tests, Quantitative

300-190-CGE 1 kit
EUR 854.4

Dog IgG ELISA Kit, 96 tests, Quantitative

400-100-DGG 1 Kit
EUR 781.2

Dog IgM ELISA Kit, 96 tests, Quantitative

400-170-DGM 1 Kit
EUR 781.2

Dog IgA ELISA Kit, 96 tests, Quantitative

400-180-DGA 1 Kit
EUR 854.4

Dog IgE ELISA Kit, 96 tests, Quantitative

400-190-DGE 1 Kit
EUR 854.4

Oxytocin ELISA Kit, 96 tests, Quantitative

10790 1 Kit
EUR 927.6

Goat IgM ELISA Kit, 96 tests, Quantitative

7530 1 kit
EUR 927.6

Goat IgA ELISA Kit, 96 tests, Quantitative

7540 1 kit
EUR 1000.8

Goat IgE ELISA Kit, 96 tests, Quantitative

7550 1 kit
EUR 1000.8

Rat IgG1 ELISA Kit, 96 tests, Quantitative

6430 1 Kit
EUR 854.4

Rat VEGF ELISA Kit, 96 tests, quantitative

200-840-VER 1 kit
EUR 927.6

Duck Pasteurellosis ELISA Kit(Quantitative)

SL0014Du -
EUR 528

Sheep IgM ELISA Kit, 96 tests, Quantitative

7630 1 kit
EUR 854.4

Sheep IgA ELISA Kit, 96 tests, Quantitative

7640 1 kit
EUR 1000.8

Sheep IgE ELISA Kit, 96 tests, Quantitative

7650 1 kit
EUR 1000.8

Horse IgG ELISA Kit, 96 tests, Quantitative

7730 1 kit
EUR 854.4

Horse IgM ELISA Kit, 96 tests, Quantitative

7740 1 kit
EUR 854.4

Horse IgE ELISA Kit, 96 tests, Quantitative

7750 1 kit
EUR 927.6

Mouse IgA ELISA Kit, 96 tests, Quantitative

6310 1 kit
EUR 781.2

Mouse IgG ELISA Kit, 96 tests, Quantitative

6320 1 kit
EUR 781.2

Mouse IgE ELISA Kit, 96 tests, Quantitative

6370 1 Kit
EUR 781.2

Mouse IgM ELISA Kit, 96 tests, Quantitative

6380 1 Kit
EUR 781.2

Rat IgG2a ELISA Kit, 96 tests, Quantitative

6440 1 Kit
EUR 578.4

Rat IgG2b ELISA Kit, 96 tests, Quantitative

6450 1 Kit
EUR 634.8

Human IgG ELISA Kit, 96 tests, Quantitative

1750 1 Kit
EUR 781.2

Human IgM ELISA Kit, 96 tests, Quantitative

1760 1 Kit
EUR 781.2

Human IgA ELISA Kit, 96 tests, Quantitative

1780 1 kit
EUR 781.2

SARS-COV-2 IgG ELISA Kit (Quantitative)

DEIASL019Q 96T
EUR 1039.92
Description: Serum, plasma

SARS-COV-2 IgM ELISA Kit (Quantitative)

DEIASL020Q 96T
EUR 1039.92
Description: Serum, plasma

Biotin Quantitative Determination ELISA Kit

E-IR-R501-24Tests 24 Tests
EUR 150

Biotin Quantitative Determination ELISA Kit

E-IR-R501-48Tests 48 Tests
EUR 288

Biotin Quantitative Determination ELISA Kit

E-IR-R501-96Tests 96 Tests
EUR 320

Biotin Quantitative Determination ELISA Kit

E-IR-R501-96Tests10 96 Tests *10
EUR 3200

Biotin Quantitative Determination ELISA Kit

E-IR-R501-96Tests5 96 Tests *5
EUR 1600

Mouse parvovirus (PV) ELISA Kit(Quantitative )

SL0660Mo -
EUR 528

Benedicts Solution Quantitative

RRSP900-D 500ml
EUR 6.36

Benedicts Solution Quantitative

RRSP900-E 1L
EUR 10.59

Benedicts Solution Quantitative

RRSP900-F 2.5L
EUR 21.96

Horse Fab2 ELISA Kit, 96 tests, Quantitative

7710-Fab 1 kit
EUR 854.4

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