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Multiplex TaqMan Real-Time PCR Assay for Sensitive Detection of Two Weevil Species (Coleoptera: Curculionidae)

Can quantitative RT-PCR for SARS-CoV-2 help in better management of patients and control of coronavirus disease 2019 pandemic

The emergence of SARS-CoV-2, the causative agent of coronavirus disease 2019 (COVID-19), represents a public health emergency of unprecedented proportion. The global containment efforts have been focused on testing, tracing of contacts and treatment (isolation) of those found COVID-19 positive. Since the whole genome sequences of a number of strains of this novel RNA virus were available in the public domain by early January 2020, a number of real-time polymerase chain reaction (RT-PCR) protocols were designed and used for diagnosis of this infection.
Most RT-PCRs are designed for qualitative COVID-19 reporting (SARS-CoV-2 detected or not detected), but have been used for semi-quantitative estimation of viral load based on cycle threshold value. Our manuscript discusses the utility of quantitative PCR testing for COVID-19 and its patient management benefits.

Multiplex TaqMan Real-Time PCR Assay for Sensitive Detection of Two Weevil Species (Coleoptera: Curculionidae)

Rapid and cost-efficient identification of Naupactus species is becoming a key process for the exportation of citrus fruit from Chile and other countries, considering the quarantine regulations for some species of the cosmopolitan genus Naupactus.
This study deals with the development of a fast and sensitive detection protocol for Naupactus cervinus (Coleoptera: Curculionidae) (Boheman) and Naupactus xanthographus (Coleoptera: Curculionidae) (Germar) based on multiplex TaqMan Real-time polymerase chain reaction. Both N. cervinus and N. xanthographus primer and probe sets achieved species-specific detection in a linear range from 1 pg/μl to 1 × 10-6 pg/μl, allowing detection of as few as 160 copies of template DNA. Non-target amplifications were not detected and a panel composed of 480 test samples had 100% coincidence with the respective morphological identification.

Validation of swab sampling and SYBR Green-based real-time PCR for the diagnosis of Cutaneous Leishmaniasis in French Guiana

 

Recent studies have highlighted the interest of non-invasive sampling procedures coupled to real-time PCR methods for detection of Leishmania species in South America. In French Guiana, sampling method still relied on skin biopsies. Non-invasive protocols should be tested on a large annual cohort to improve routine laboratory diagnosis of Cutaneous Leishmaniasis.
Therefore, we evaluated the performances of a new Leishmania detection and species identification protocol involving cotton swabs and SYBR Green real-time PCR of Hsp70 gene, coupled with Sanger sequencing. Between May 2017 and May 2018, 145 patients with ulcerated lesions compatible with Cutaneous Leishmaniasis were included in the Cayenne Hospital and its remote health centres. Each patient underwent scrapings for smear, skin biopsies for parasite culture and PCR-RFLP (RNA pol II) and cotton swabs for SYBR Green PCR. The most accurate diagnostic test was the SYBR Green PCR on swab sampling, showing a 98% sensitivity.
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SYBR Green qPCR Master Mix (No ROX)

C0006-1g 1g Ask for price
Description: SYBR Green qPCR Master Mix (No ROX)

SYBR Green qPCR Master Mix (No ROX)

C0006-1mg 1mg Ask for price
Description: SYBR Green qPCR Master Mix (No ROX)

SYBR Green qPCR Master Mix (No ROX)

C0006-50mg 50mg Ask for price
Description: SYBR Green qPCR Master Mix (No ROX)

SYBR Green qPCR Master Mix (No ROX)

C0006-5mg 5mg Ask for price
Description: SYBR Green qPCR Master Mix (No ROX)

SYBR Green qPCR Master Mix (No ROX)

HY-K0523 5 mL (500 rxns )
EUR 308.4

SYBR Green qPCR Master Mix (Low ROX)

C0008-10mg 10mg Ask for price
Description: SYBR Green qPCR Master Mix (Low ROX)

SYBR Green qPCR Master Mix (Low ROX)

C0008-1g 1g Ask for price
Description: SYBR Green qPCR Master Mix (Low ROX)

SYBR Green qPCR Master Mix (Low ROX)

C0008-1mg 1mg Ask for price
Description: SYBR Green qPCR Master Mix (Low ROX)

SYBR Green qPCR Master Mix (Low ROX)

C0008-50mg 50mg Ask for price
Description: SYBR Green qPCR Master Mix (Low ROX)

SYBR Green qPCR Master Mix (Low ROX)

C0008-5mg 5mg Ask for price
Description: SYBR Green qPCR Master Mix (Low ROX)

SYBR Green qPCR Master Mix (Low ROX)

HY-K0522 5 mL (500 rxns )
EUR 308.4

SYBR Green qPCR Master Mix (High ROX)

C0007-10mg 10mg Ask for price
Description: SYBR Green qPCR Master Mix (High ROX)

SYBR Green qPCR Master Mix (High ROX)

C0007-1g 1g Ask for price
Description: SYBR Green qPCR Master Mix (High ROX)

SYBR Green qPCR Master Mix (High ROX)

C0007-1mg 1mg Ask for price
Description: SYBR Green qPCR Master Mix (High ROX)

SYBR Green qPCR Master Mix (High ROX)

C0007-50mg 50mg Ask for price
Description: SYBR Green qPCR Master Mix (High ROX)

SYBR Green qPCR Master Mix (High ROX)

C0007-5mg 5mg Ask for price
Description: SYBR Green qPCR Master Mix (High ROX)

SYBR Green qPCR Master Mix (High ROX)

HY-K0521 1 mL (100 rxns)
EUR 135.6

2x SYBR Green qPCR Master Mix (Low ROX)

B21702 5 ml
EUR 268.8
Description: 2x SYBR Green qPCR master mix (Low ROX) utilizes a special performance-enhanced Taq DNA polymerase protected via a hot-start activation technique, and optimized qPCR buffer system to perform SYBR

2x SYBR Green qPCR Master Mix (Low ROX)

B21703 25 ml
EUR 1027.2
Description: 2x SYBR Green qPCR master mix (Low ROX) utilizes a special performance-enhanced Taq DNA polymerase protected via a hot-start activation technique, and optimized qPCR buffer system to perform SYBR

2x SYBR Green qPCR Master Mix (High ROX)

B21402 5 ml
EUR 268.8
Description: 2x SYBR Green qPCR master mix (High ROX) utilizes a special performance-enhanced Taq DNA polymerase protected via a hot-start activation technique, and optimized qPCR buffer system to perform SYBR

2x SYBR Green qPCR Master Mix (High ROX)

B21403 25 ml
EUR 1027.2
Description: 2x SYBR Green qPCR master mix (High ROX) utilizes a special performance-enhanced Taq DNA polymerase protected via a hot-start activation technique, and optimized qPCR buffer system to perform SYBR

AceQ qPCR SYBR® Green Master Mix

Q111-02 500 rxn (20 μl/rxn)
EUR 265.2

AceQ qPCR SYBR® Green Master Mix

Q111-03 2500 rxn (20 μl/rxn)
EUR 775.2

HotStart™ 2X SYBR Green qPCR Master Mix

K1070-25 25ml
EUR 336

HotStart™ 2X SYBR Green qPCR Master Mix

K1070-5 5ml
EUR 96

HotStart™ 2X SYBR Green qPCR Master Mix

K1070-50 50ml
EUR 672

ig SYBR Green qPCR 2X Master Mix - 200 Reactions

3354 200
EUR 110
Description: Intact Genomics SYBR Green qPCR 2X Master Mix master mix is a ready-to-use cocktail containing all components except primers and template, for the amplification and detection of DNA in qPCR. The Ig SYBR® Green qPCR 2x master mix with integrated chemically-modified hot start Taq DNA polymerase, SYBR® Green I fluorescent dye, ROX dye, MgCl2, dNTPs and stabilizers. This master mix is ideal for high-throughput real-time PCR screening and validation. The amplification step features a high quality hot start Taq DNA Polymerase which offers higher fidelity and better amplification.

HotStart™ Universal 2X SYBR Green qPCR Master Mix

K1170-25 25ml
EUR 336
Description: qPCR Kit, Universal SYBR Green I Master Mix (Rox)

HotStart™ Universal 2X SYBR Green qPCR Master Mix

K1170-5 5ml
EUR 96
Description: qPCR Kit, Universal SYBR Green I Master Mix (Rox)

HotStart™ Universal 2X SYBR Green qPCR Master Mix

K1170-50 50ml
EUR 672
Description: qPCR Kit, Universal SYBR Green I Master Mix (Rox)

ChamQ SYBR qPCR Master Mix

Q311-02 500 rxns (20 μl/rxn)
EUR 120.55

ChamQ SYBR qPCR Master Mix

Q311-03 2,500 rxns (20 μl/rxn)
EUR 543.5

Apex qPCR GREEN Master Mix

42-116PG 400 x 25µl Reactions/Unit
EUR 341.44
Description: 2X, Without ROX, 5ml

Apex qPCR GREEN Master Mix

42-117PG 4000 x 25µl Reactions/Unit
EUR 2926.74
Description: 2X, Without ROX, 50ml

Apex qPCR GREEN Master Mix

42-118PG 400 x 25µl Reactions/Unit
EUR 338.88
Description: 2X, Low ROX, 5ml

Apex qPCR GREEN Master Mix

42-119PG 4000 x 25µl Reactions/Unit
EUR 2926.74
Description: 2X, Low ROX, 50ml

Apex qPCR GREEN Master Mix

42-120PG 400 x 25µl Reactions/Unit
EUR 341.44
Description: 2X, High ROX, 5ml

Apex qPCR GREEN Master Mix

42-121PG 4000 x 25µl Reactions/Unit
EUR 3095.57
Description: 2X, High ROX, 50ml

ChamQ SYBR Color qPCR Master Mix

Q411-02 500 rxns (20 μl/rxn)
EUR 140.63

ChamQ SYBR Color qPCR Master Mix

Q411-03 2,500 rxns (20 μl/rxn)
EUR 634

AceQ Universal SYBR qPCR Master Mix

Q511-02 500 rxn (20 μl/rxn)
EUR 152.72

AceQ Universal SYBR qPCR Master Mix

Q511-03 2500 rxn (20 μl/rxn)
EUR 688.5

miRNA Universal SYBR qPCR Master Mix

MQ101-01 125 rxn(20 μl/rxn)
EUR 38.9

miRNA Universal SYBR qPCR Master Mix

MQ101-02 500 rxn(20 μl/rxn)
EUR 145

ChamQ Universal SYBR qPCR Master Mix

Q711-02 500 rxn (20 μl/rxn)
EUR 152.72

ChamQ Universal SYBR qPCR Master Mix

Q711-03 2500 rxn (20 μl/rxn)
EUR 688.5

ChamQ SYBR qPCR Master Mix (Without ROX)

Q321-02 500 rxns (20 μl/rxn)
EUR 120.55

ChamQ SYBR qPCR Master Mix (Without ROX)

Q321-03 2,500 rxns (20 μl/rxn)
EUR 543.5

Taq Pro Universal SYBR qPCR Master Mix

Q712-02 500 rxns (20 μl/rxn)
EUR 161

Taq Pro Universal SYBR qPCR Master Mix

Q712-03 2,500 rxns (20 μl/rxn)
EUR 725

ViPrimePLUSHigh 5X One Step RT-qPCR Green Master Mix I (SYBR® Green Dye), 100rxns

QLMM10 each Ask for price

ChamQ SYBR qPCR Master Mix (Low ROX Premixed)

Q331-02 500 rxns (20 μl/rxn)
EUR 120.55

ChamQ SYBR qPCR Master Mix (Low ROX Premixed)

Q331-03 2,500 rxns (20 μl/rxn)
EUR 543.5

ChamQ SYBR qPCR Master Mix (High ROX Premixed)

Q341-02 500 rxns (20 μl/rxn)
EUR 120.55

ChamQ SYBR qPCR Master Mix (High ROX Premixed)

Q341-03 2,500 rxns (20 μl/rxn)
EUR 543.5

ChamQ SYBR Color qPCR Master Mix (Without ROX)

Q421-02 500 rxns (20 μl/rxn)
EUR 140.63

ChamQ SYBR Color qPCR Master Mix (Without ROX)

Q421-03 2,500 rxns (20 μl/rxn)
EUR 634

ViPrimePLUS Taq qPCR Green Master Mix I, 150rxns

QLMM12 each Ask for price

ViPrimePLUS Taq qPCR Green Master Mix II, 150rxns

QLMM17 each Ask for price

ViPrimePLUS AtTaq qPCR Green Master Mix I, 150rxns

QLMM02 each Ask for price

ViPrimePLUS AtTaq qPCR Green Master Mix II, 150rxns

QLMM07 each Ask for price

ChamQ SYBR Color qPCR Master Mix (Low ROX Premixed)

Q431-02 500 rxns (20 μl/rxn)
EUR 140.63

ChamQ SYBR Color qPCR Master Mix (Low ROX Premixed)

Q431-03 2,500 rxns (20 μl/rxn)
EUR 634

ChamQ SYBR Color qPCR Master Mix (High ROX Premixed)

Q441-02 500 rxns (20 μl/rxn)
EUR 140.63

ChamQ SYBR Color qPCR Master Mix (High ROX Premixed)

Q441-03 2,500 rxns (20 μl/rxn)
EUR 634

TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green *No ROX*

17270 1 mL
EUR 203
Description: TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR.

TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green *No ROX*

17270-1mL 1 mL
EUR 99
Description: TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR.

TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green *No ROX*

17271 5 mL
EUR 508
Description: TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR.

TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green *No ROX*

17271-5mL 5 mL
EUR 308
Description: TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR.

TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green *Low ROX*

17272 1 mL
EUR 203
Description: TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR.

TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green *Low ROX*

17272-1mL 1 mL
EUR 99
Description: TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR.

TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green *Low ROX*

17273 5 mL
EUR 508
Description: TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR.

TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green *Low ROX*

17273-5mL 5 mL
EUR 308
Description: TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR.

TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green *High ROX*

17274 1 mL
EUR 203
Description: TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR.

TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green *High ROX*

17274-1mL 1 mL
EUR 99
Description: TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR.

TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green *High ROX*

17275 5 mL
EUR 508
Description: TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR.

TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green *High ROX*

17275-5mL 5 mL
EUR 308
Description: TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR.

TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green *No ROX*

17276 1 mL
EUR 203
Description: TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR.

TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green *No ROX*

17276-1mL 1 mL
EUR 99
Description: TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR.

TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green *No ROX*

17277 5 mL
EUR 508
Description: TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR.

TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green *No ROX*

17277-5mL 5 mL
EUR 308
Description: TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR.

TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green *Low ROX*

17278 1 mL
EUR 203
Description: TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR.

TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green *Low ROX*

17278-1mL 1 mL
EUR 99
Description: TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR.

TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green *Low ROX*

17279 5 mL
EUR 508
Description: TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR.

TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green *Low ROX*

17279-5mL 5 mL
EUR 308
Description: TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR.

TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green *High ROX*

17280 1 mL
EUR 203
Description: TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR.

TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green *High ROX*

17280-1mL 1 mL
EUR 99
Description: TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR.

TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green *High ROX*

17281 5 mL
EUR 508
Description: TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR.

TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green *High ROX*

17281-5mL 5 mL
EUR 308
Description: TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR.

Genious 2X SYBR Green Fast qPCR Mix

RK21204 1 mL
EUR 328.5

2X Universal SYBR Green Fast qPCR Mix

RK21203 1 mL
EUR 76.5

ViPrimePLUS Taq qPCR Green Master Mix I with ROX, 150rxns

QLMM12-R each Ask for price

ViPrimePLUS Taq qPCR Green Master Mix II with ROX, 150rxns

QLMM17-R each Ask for price

ViPrimePLUS AtTaq qPCR Green Master Mix I with ROX, 150rxns

QLMM02-R each Ask for price

ViPrimePLUS AtTaq qPCR Green Master Mix II with ROX, 150rxns

QLMM07-R each Ask for price

lyophilised real-time PCR Master Mix with SYBR® Green fluorescent DNA stain

M-PCR-173L 960 reactions x 20 µl
EUR 897
Description: lyophilised real-time PCR Master Mix with SYBR® Green fluorescent DNA stain

lyophilised real-time PCR Master Mix with SYBR® Green fluorescent DNA stain

M-PCR-173S 192 reactions x 20 µl
EUR 224
Description: lyophilised real-time PCR Master Mix with SYBR® Green fluorescent DNA stain

ViPrimePLUS Taq qPCR Green Master Mix I with Low ROX, 150rxns

QLMM12-LR each Ask for price

ViPrimePLUS Taq qPCR Green Master Mix II with Low ROX, 150rxns

QLMM17-LR each Ask for price

BrightCycle Universal SYBR Green qPCR Mix with UDG

RK21219 5mL
EUR 475

ViPrimePLUS AtTaq qPCR Green Master Mix I with Low ROX, 150rxns

QLMM02-LR each Ask for price

ViPrimePLUS AtTaq qPCR Green Master Mix II with Low ROX, 150rxns

QLMM07-LR each Ask for price

PCR Master mix (SYBR)

abx460031-1096tests 10 × 96 tests Ask for price

PCR Master mix (SYBR)

abx460031-596tests 5 × 96 tests Ask for price

PCR Master mix (SYBR)

abx460031-96tests 96 tests
EUR 337.5

Genious 2X SYBR Green Fast qPCR Mix (No ROX)

RK21205 1 ml
EUR 328.5

qPCR Master Mix

abx460014-1096tests 10 × 96 tests Ask for price

qPCR Master Mix

abx460014-596tests 5 × 96 tests Ask for price

qPCR Master Mix

abx460014-96tests 96 tests
EUR 337.5

1ml PrecisionPLUS qPCR Master Mix premixed with SYBRgreen

Z-PPLUS-SY-1ML 1ml
EUR 81
Description: Precision®PLUS

Genious 2X SYBR Green Fast qPCR Mix (Low ROX Premixed)

RK21206 1 ml
EUR 328.5

Genious 2X SYBR Green Fast qPCR Mix (High ROX Premixed)

RK21207 1 ml
EUR 93

1ml Precision FAST qPCR Master Mix premixed with SYBRgreen

Z-PFAST-SY-1ML 1ml
EUR 90
Description: Precision®FAST

2ml Precision FAST qPCR Master Mix premixed with SYBRgreen

Z-PFAST-SY-2ML 2ml
EUR 161
Description: Precision®FAST

amfiSure Ultra Fidelity PCR Master Mix(2X)

P0346-001 1ml
EUR 336

amfiSure Ultra Fidelity PCR Master Mix(2X)

P0346-002 2x1ml
EUR 578.4

amfiSure Ultra Fidelity PCR Master Mix(2X)

P0346-005 5x1ml
EUR 993.6

amfiSure Ultra Fidelity PCR Master Mix(2X)

P0346-010 10x1ml
EUR 1632

ViPrimePLUS OneStep Taq RT-qPCR Green Master Mix I, 150rxns

QLMM14 each Ask for price

ViPrimePLUS OneStep Taq RT-qPCR Green Master Mix II, 150rxns

QLMM18 each Ask for price
Mean PCR cycle threshold (Ct) was of 24.4 Ct (min=17 Ct, max=36 Ct) and was inferior to 35 Ct in 97.6% of samples. All samples positive with real-time PCR SYBR Green were successfully identified at the species level by DNA sequencing. This new method should be considered for routine diagnosis of Cutaneous Leishmaniasis in South America and especially for remote areas, as non-invasive collection tools are easier to use and require less precautions for transportation.

Development and clinical validation of a pangenotypic PCR-based assay for the detection and quantification of hepatitis E virus (Orthopevirus A genus)

 

The objective of this study was to design a pangenotypic PCR-based assay for the detection and quantification of hepatitis E virus (HEV) RNA from across the entire spectrum of described genotypes belonging to the Orthohepevirus A genus. The optimal conditions and the performance of the assay were determined by testing the WHO standard strain (6219/10) and the WHO HEV panel (8578/13).
Similarly, performance comparisons were made with two commercial assays (Real Star HEV RT-PCR 2.0 and ampliCube HEV 2.0 Quant) to detect HEV RNA at concentrations below 1,000 IU/mL with viral strains from the WHO and to test samples from 54 patients with acute hepatitis. The assay presented in this study was able to detect the entire spectrum of described genotypes belonging to the Orthohepevirus A genus, demonstrating a better performance than both commercial kits. This procedure may represent a significant improvement in the molecular diagnosis of HEV infection.

SARS-CoV-2 and RT-PCR in asymptomatic patients: Results of a cohort of workers at El Dorado International Airport in Bogotá, 2020

 

Introduction: The 2019 coronavirus pandemic (COVID-19) has caused around 25 million cases worldwide. Asymptomatic patients have been described as potential sources of transmission. However, there are difficulties to detect them and to establish their role in the dynamics of virus transmission, which hinders the implementation of prevention strategies. Objective: To describe the behavior of asymptomatic SARS-CoV-2 virus infection in a cohort of workers at the El Dorado “Luis Carlos Galán Sarmiento” International Airport in Bogotá, Colombia. Materials and methods: A prospective cohort of 212 workers from the El Dorado airport was designed. The follow-up began in June, 2020. A survey was used to characterize health and work conditions.
Every 21 day, a nasopharyngeal swab was taken to identify the presence of SARS-CoV-2 using RT-PCR. We analyzed the behavior of the cycle threshold (ORF1ab and N genes) according to the day of follow-up. Results: In the first three follow-ups of the cohort, we found an incidence of SARS-CoV-2 infection of 16.51%. The proportion of positive contacts was 14.08%. The median threshold for cycle threshold was 33.53. Conclusion: We characterized the asymptomatic SARS-CoV-2 infection in a cohort of workers. The identification of asymptomatic infected persons continues to be a challenge for epidemiological surveillance systems.
Introducción. La pandemia de COVID-19 ha ocasionado cerca de 25 millones de casos en el mundo. Se ha descrito que los pacientes asintomáticos pueden ser fuentes de transmisión. Sin embargo, es difícil detectarlos y no es claro su papel en la dinámica de transmisión del virus, lo que obstaculiza la implementación de estrategias para la prevención.
Objetivo. Describir el comportamiento de la infección asintomática por SARS-CoV-2 en una cohorte de trabajadores del Aeropuerto Internacional El Dorado “Luis Carlos Galán Sarmiento” de Bogotá, Colombia. Materiales y métodos. Se diseñó una cohorte prospectiva de trabajadores del Aeropuerto El Dorado. El seguimiento se inició en junio de 2020 con una encuesta a cada trabajador para caracterizar sus condiciones de salud y trabajo. Cada 21 días se tomó una muestra de hisopado nasofaríngeo para detectar la presencia del SARS-CoV-2 mediante reacción en cadena de la polimerasa con transcriptasa inversa (RT-PCR). Se analizó el comportamiento del umbral del ciclo (cycle threshold) de los genes ORF1ab y N según el día de seguimiento.
Resultados. En los primeros tres seguimientos de la cohorte se encontró una incidencia de la infección por SARS-CoV-2 del 16,51 %. La proporción de contactos positivos fue del 14,08 %. La mediana del umbral del ciclo fue de 33,53. Conclusión. Se determinaron las características de la infección asintomática por el SARSCoV-2 en una cohorte de trabajadores. La detección de infectados asintomáticos sigue siendo un reto para los sistemas de vigilancia epidemiológica.

Green Dye One Step qRT-PCR Master Mix For Quantitative Real Time PCR With ROX Dye

MB1004-100Reactions 100 Reactions
EUR 392.4

EpiQuik Quantitative PCR Fast Kit 

P-1029
  • Ask for price
  • Ask for price
  • Ask for price
  • 200 Reactions
  • 100 Reactions
  • 200 Reactions

Rat Real-time PCR DNA Quantitation after QPCR DNA Damage Analysis Kit

DD2R 1 Kit
EUR 550

Human Real-time PCR DNA Quantitation after QPCR DNA Damage Analysis Kit

DD2H 1 Kit
EUR 550

Mouse Real-time PCR DNA Quantitation after QPCR DNA Damage Analysis Kit

DD2M 1 Kit
EUR 550

qPCR ProbesMasterMaster mix for quantitative real-time PCR using labeled DNA probes, 2 x conc. master mix** shipped ice packs - must be shipped via overnight service

M-PCR-360L 10 x 1,25 ml
EUR 818
Description: qPCR ProbesMasterMaster mix for quantitative real-time PCR using labeled DNA probes, 2 x conc. master mix** shipped ice packs - must be shipped via overnight service

qPCR ProbesMasterMaster mix for quantitative real-time PCR using labeled DNA probes, 2 x conc. master mix** shipped ice packs - must be shipped via overnight service

M-PCR-360S 2 x 1,25 ml
EUR 205
Description: qPCR ProbesMasterMaster mix for quantitative real-time PCR using labeled DNA probes, 2 x conc. master mix** shipped ice packs - must be shipped via overnight service

qPCR ProbesMaster UNGMaster mix for quantitative real-time PCR using labeled DNA probes, 2 x conc. master mix** shipped ice packs - must be shipped via overnight service

M-PCR-363L 10 x 1,25 ml
EUR 982
Description: qPCR ProbesMaster UNGMaster mix for quantitative real-time PCR using labeled DNA probes, 2 x conc. master mix** shipped ice packs - must be shipped via overnight service

qPCR ProbesMaster UNGMaster mix for quantitative real-time PCR using labeled DNA probes, 2 x conc. master mix** shipped ice packs - must be shipped via overnight service

M-PCR-363S 2 x 1,25 ml
EUR 246
Description: qPCR ProbesMaster UNGMaster mix for quantitative real-time PCR using labeled DNA probes, 2 x conc. master mix** shipped ice packs - must be shipped via overnight service

qPCR ProbesMaster highROXMaster mix for quantitative real-time PCR using labeled DNA probes, 2x conc. master mix** shipped ice packs - must be shipped via overnight service

M-PCR-362L 10 x 1,25 ml
EUR 818
Description: qPCR ProbesMaster highROXMaster mix for quantitative real-time PCR using labeled DNA probes, 2x conc. master mix** shipped ice packs - must be shipped via overnight service

qPCR ProbesMaster highROXMaster mix for quantitative real-time PCR using labeled DNA probes, 2x conc. master mix** shipped ice packs - must be shipped via overnight service

M-PCR-362S 2 x 1,25 ml
EUR 205
Description: qPCR ProbesMaster highROXMaster mix for quantitative real-time PCR using labeled DNA probes, 2x conc. master mix** shipped ice packs - must be shipped via overnight service

qPCR ProbesMaster lowROXMaster mix for quantitative real-time PCR using labeled DNA probes, 2 x conc. master mix** shipped ice packs - must be shipped via overnight service

M-PCR-361L 10 x 1,25 ml
EUR 818
Description: qPCR ProbesMaster lowROXMaster mix for quantitative real-time PCR using labeled DNA probes, 2 x conc. master mix** shipped ice packs - must be shipped via overnight service

qPCR ProbesMaster lowROXMaster mix for quantitative real-time PCR using labeled DNA probes, 2 x conc. master mix** shipped ice packs - must be shipped via overnight service

M-PCR-361S 2 x 1,25 ml
EUR 205
Description: qPCR ProbesMaster lowROXMaster mix for quantitative real-time PCR using labeled DNA probes, 2 x conc. master mix** shipped ice packs - must be shipped via overnight service

qPCR ProbesMaster UNG lowROXMaster mix for quantitative real-time PCR using labeled DNA probes, 2 x conc. master mix** shipped ice packs - must be shipped via overnight service

M-PCR-364L 10 x 1,25 ml
EUR 982
Description: qPCR ProbesMaster UNG lowROXMaster mix for quantitative real-time PCR using labeled DNA probes, 2 x conc. master mix** shipped ice packs - must be shipped via overnight service

qPCR ProbesMaster UNG lowROXMaster mix for quantitative real-time PCR using labeled DNA probes, 2 x conc. master mix** shipped ice packs - must be shipped via overnight service

M-PCR-364S 2 x 1,25 ml
EUR 246
Description: qPCR ProbesMaster UNG lowROXMaster mix for quantitative real-time PCR using labeled DNA probes, 2 x conc. master mix** shipped ice packs - must be shipped via overnight service

qPCR ProbesMaster UNG highROXMaster mix for quantitative real-time PCR using labeled DNA probes, 2 x conc. master mix** shipped ice packs - must be shipped via overnight service

M-PCR-365L 10 x 1,25 ml
EUR 982
Description: qPCR ProbesMaster UNG highROXMaster mix for quantitative real-time PCR using labeled DNA probes, 2 x conc. master mix** shipped ice packs - must be shipped via overnight service

qPCR ProbesMaster UNG highROXMaster mix for quantitative real-time PCR using labeled DNA probes, 2 x conc. master mix** shipped ice packs - must be shipped via overnight service

M-PCR-365S 2 x 1,25 ml
EUR 246
Description: qPCR ProbesMaster UNG highROXMaster mix for quantitative real-time PCR using labeled DNA probes, 2 x conc. master mix** shipped ice packs - must be shipped via overnight service

Benedict’s Quantitative Reagent

R003-500ML 1 unit
EUR 7.09
Description: Benedict’s Quantitative Reagent

RT-PCR quantitative Covid-19 assay CE-IVD

CLO-RT-25 48 reactions
EUR 2513
Description: RT-PCR quantitative Covid-19 assay CE-IVD

Tiamulin ELISA Kit, Quantitative

DEIA036 96T
EUR 1414.8
Description: This kit is intended for quantitative analysis of tiamulin residue in animal tissue (pork and chicken, etc).

Fumonisin Quantitative Test Kit

Q603 96T
EUR 200
Description: Feed Test

T2 toxin Quantitative Test Kit

Q604 96T
EUR 200
Description: Feed Test

Zearalenone Quantitative Test Kit

Q601 96T
EUR 200
Description: Feed Test

HBV Genetype B,C&D Real Time PCR Kit

GWB-LRB023 25 tests Ask for price

HBV Genetype B,C&D Real Time PCR Kit

GWB-LRB024 25 tests Ask for price

HBV Adefovir-resistant Mutants Real Time PCR Kit

GWB-LRB019 25 tests Ask for price

HBV Adefovir-resistant Mutants Real Time PCR Kit

GWB-LRB020 25 tests Ask for price

Human RBP4 Quantitative ELISA Kit

LF-EK70030 1×96T
EUR 722.4

Aflatoxin B1 Quantitative Test Kit

Q401 96T
EUR 200
Description: Feed Test

Ochratoxin A Quantitative Test Kit

Q605 96T
EUR 200
Description: Feed Test

DNA Oxidative Damage Kit - Quantitative

DN-002 20 tests
EUR 656

HBV Precore Site 1896 Mutation Real Time PCR Kit

GWB-LRB021 25 tests Ask for price

HBV Precore Site 1896 Mutation Real Time PCR Kit

GWB-LRB022 25 tests Ask for price

Mouse NoroVirus ELISA Kit(Quantitative )

SL0661Mo -
EUR 528

Human Mumps IgG ELISA Kit (quantitative)

SL3197Hu 96 Tests
EUR 468

Rat C3 ELISA Kit, 96 tests, Quantitative

6230 1 kit
EUR 854.4

Mouse Mycoplasma Elisa kit(quantitative)

EK730424 96 Wells
EUR 0.68

Lactate ELISA Kit, 96 tests, Quantitative

10795 1 Kit
EUR 927.6

Pig IgM ELISA Kit, 96 tests, Quantitative

9080 1 kit
EUR 854.4

Rat IgA ELISA kit 96 tests, Quantitative

6410-10 1 kit
EUR 607.2

Rat IgG ELISA Kit, 96 tests, Quantitative

6420 1 kit
EUR 781.2

Rat IgE ELISA Kit, 96 tests, Quantitative

6470 1 kit
EUR 927.6

Rat IgM ELISA Kit, 96 tests, Quantitative

6480 1 Kit
EUR 854.4

Cat IgG ELISA Kit, 96 tests, Quantitative

300-100-CGG 1 kit
EUR 854.4

Cat IgM ELISA kit 96 tests, Quantitative

300-170-CGM 1 kit
EUR 854.4

Cat IgA ELISA Kit, 96 tests, Quantitative

300-180-CGA 1 kit
EUR 854.4

Cat IgE ELISA Kit, 96 tests, Quantitative

300-190-CGE 1 kit
EUR 854.4

Dog IgG ELISA Kit, 96 tests, Quantitative

400-100-DGG 1 Kit
EUR 781.2

Dog IgM ELISA Kit, 96 tests, Quantitative

400-170-DGM 1 Kit
EUR 781.2

Dog IgA ELISA Kit, 96 tests, Quantitative

400-180-DGA 1 Kit
EUR 854.4

Dog IgE ELISA Kit, 96 tests, Quantitative

400-190-DGE 1 Kit
EUR 854.4

TBE / FSME IgG quantitative

GWB-AC53EA 1x96 Assays Ask for price

Oxytocin ELISA Kit, 96 tests, Quantitative

10790 1 Kit
EUR 927.6

Goat IgM ELISA Kit, 96 tests, Quantitative

7530 1 kit
EUR 927.6

Goat IgA ELISA Kit, 96 tests, Quantitative

7540 1 kit
EUR 1000.8

Goat IgE ELISA Kit, 96 tests, Quantitative

7550 1 kit
EUR 1000.8

Rat IgG1 ELISA Kit, 96 tests, Quantitative

6430 1 Kit
EUR 854.4

Rat VEGF ELISA Kit, 96 tests, quantitative

200-840-VER 1 kit
EUR 927.6

Duck Pasteurellosis ELISA Kit(Quantitative)

SL0014Du -
EUR 528

Sheep IgM ELISA Kit, 96 tests, Quantitative

7630 1 kit
EUR 854.4

Sheep IgA ELISA Kit, 96 tests, Quantitative

7640 1 kit
EUR 1000.8

Sheep IgE ELISA Kit, 96 tests, Quantitative

7650 1 kit
EUR 1000.8

Horse IgG ELISA Kit, 96 tests, Quantitative

7730 1 kit
EUR 854.4

Horse IgM ELISA Kit, 96 tests, Quantitative

7740 1 kit
EUR 854.4

Horse IgE ELISA Kit, 96 tests, Quantitative

7750 1 kit
EUR 927.6

Mouse IgA ELISA Kit, 96 tests, Quantitative

6310 1 kit
EUR 781.2

Mouse IgG ELISA Kit, 96 tests, Quantitative

6320 1 kit
EUR 781.2

Mouse IgE ELISA Kit, 96 tests, Quantitative

6370 1 Kit
EUR 781.2

Mouse IgM ELISA Kit, 96 tests, Quantitative

6380 1 Kit
EUR 781.2

Rat IgG2a ELISA Kit, 96 tests, Quantitative

6440 1 Kit
EUR 578.4

Rat IgG2b ELISA Kit, 96 tests, Quantitative

6450 1 Kit
EUR 634.8

Human IgG ELISA Kit, 96 tests, Quantitative

1750 1 Kit
EUR 781.2

Human IgM ELISA Kit, 96 tests, Quantitative

1760 1 Kit
EUR 781.2

Human IgA ELISA Kit, 96 tests, Quantitative

1780 1 kit
EUR 781.2

SARS-COV-2 IgG ELISA Kit (Quantitative)

DEIASL019Q 96T
EUR 1039.92
Description: Serum, plasma

SARS-COV-2 IgM ELISA Kit (Quantitative)

DEIASL020Q 96T
EUR 1039.92
Description: Serum, plasma

Mouse parvovirus (PV) ELISA Kit(Quantitative )

SL0660Mo -
EUR 528

Biotin Quantitative Determination ELISA Kit

E-IR-R501-24Tests 24 Tests
EUR 150

Biotin Quantitative Determination ELISA Kit

E-IR-R501-48Tests 48 Tests
EUR 288

Biotin Quantitative Determination ELISA Kit

E-IR-R501-96Tests 96 Tests
EUR 320

Biotin Quantitative Determination ELISA Kit

E-IR-R501-96Tests10 96 Tests *10
EUR 3200

Biotin Quantitative Determination ELISA Kit

E-IR-R501-96Tests5 96 Tests *5
EUR 1600

G. Pig IgM ELISA Kit, 96 tests, Quantitative

7430 1 kit
EUR 781.2

Protein-A ELISA Kit, 480 tests, Quantitative

800-110-PRA-5 1 pk
EUR 3714

Horse Fab2 ELISA Kit, 96 tests, Quantitative

7710-Fab 1 kit
EUR 854.4

Mouse VEGF ELISA Kit, 96 tests, Quantitative

100-230-VEM 1 Kit
EUR 854.4

Mouse IgG1 ELISA Kit, 96 tests, Quantitative

6330 1 kit
EUR 781.2

Mouse IgG3 ELISA Kit, 96 tests, Quantitative

6360 1 kit
EUR 781.2

Rabbit IgM ELISA Kit, 96 tests, Quantitative

6580 1 kit
EUR 708

Vitamin A ELISA Kit, 96 tests, Quantitative

10760 1 Kit
EUR 927.6

Vitamin B1 ELISA Kit, 96 tests, Quantitative

10765 1 Kit
EUR 927.6

Vitamin C ELISA Kit, 96 tests, Quantitative

10770 1 Kit
EUR 927.6

Vitamin E ELISA Kit, 96 tests, Quantitative

10775 1 Kit
EUR 927.6

Turkey IgG ELISA Kit, 96 tests, Quantitative

8020 1 kit
EUR 781.2

Bovine IgA ELISA Kit, 96 tests, Quantitative

8075 1 Kit
EUR 854.4

Bovine IgM ELISA Kit, 96 tests, Quantitative

8080 1 Kit
EUR 854.4

Human CCL2 ELISA Kit, 96 tests, Quantitative

HCC-0L2-105 1 kit
EUR 578.4

Human CCL3 ELISA Kit, 96 tests, Quantitative

HCC-0L3-110 1 kit
EUR 578.4

Human CCL4 ELISA Kit, 96 tests, Quantitative

HCC-0L4-115 1 kit
EUR 578.4

Human CD93 ELISA Kit, 96 tests, Quantitative

HCD-093-120 1 kit
EUR 578.4

Mouse CCL2 ELISA Kit, 96 tests, Quantitative

MCC-0L2-100 1 kit
EUR 578.4

Mouse CCL3 ELISA Kit, 96 tests, Quantitative

MCC-0L3-100 1 kit
EUR 607.2

Vasopressin ELISA Kit, 96 tests, Quantitative

10800 1 Kit
EUR 927.6

Rat IL-13 ELISA Kit, 96 tests, Quantitative

RIL-013-100 1 kit
EUR 578.4

Mouse IgG2a ELISA Kit, 96 tests, Quantitative

6340 1 kit
EUR 781.2

Rat Albumin ELISA Kit, 96 tests, Quantitative

6400 1 kit
EUR 781.2

Vitamin B12 ELISA kit, 96 tests, Quantitative

0340-B12 1 Kit
EUR 927.6

Pig Insulin ELISA Kit, 96 tests, Quantitative

10420 1 Kit
EUR 927.6

D-Lactate ELISA Kit, 96 tests, Quantitative

10845 1 Kit
EUR 927.6

Dog Albumin ELISA Kit, 96 tests, Quantitative

7000-50 1 kit
EUR 781.2

Protein-A ELISA Kit, 96 tests, Quantitative

800-110-PRA 1 kit
EUR 927.6

Human CXCL3 ELISA Kit, 96 tests, Quantitative

HCXC-003-100 1 kit
EUR 578.4

Human CXCL9 ELISA Kit, 96 tests, Quantitative

HCXC-090-100 1 kit
EUR 578.4

Mouse CXCL3 ELISA Kit, 96 tests, Quantitative

MCXC-003-100 1 kit
EUR 578.4

Homocysteine ELISA kit, 96 tests, Quantitative

0370-HCY 1 kit
EUR 927.6

Prostacyclin ELISA Kit, 96 tests, Quantitative

10785 1 Kit
EUR 927.6

Human IL-4 ELISA Kit, 96 tests, Quantitative

HIL-004-105 1 kit
EUR 578.4

Human IL-5 ELISA Kit, 96 tests, Quantitative

HIL-005-110 1 kit
EUR 578.4

Human IL-9 ELISA Kit, 96 tests, Quantitative

HIL-009-115 1 kit
EUR 607.2

Human IL-8 ELISA Kit, 96 tests, Quantitative

HIL-080-170 1 kit
EUR 578.4

Mouse IL-2 ELISA Kit, 96 tests, Quantitative

MIL-002-105 1 kit
EUR 578.4

Mouse IL-4 ELISA Kit, 96 tests, Quantitative

MIL-004-110 1 kit
EUR 578.4

Mouse IL-5 ELISA Kit, 96 tests, Quantitative

MIL-005-115 1 kit
EUR 578.4

Mouse IL-9 ELISA Kit, 96 tests, Quantitative

MIL-009-120 1 kit
EUR 578.4

Rat IL-17A ELISA Kit, 96 tests, Quantitative

RIL-17A-100 1 kit
EUR 607.2

Rat Glucagon ELISA Kit, 96 tests, Quantitative

9485 1 Kit
EUR 927.6

Mouse Leptin ELISA Kit, 96 tests, Quantitative

100-130-LEM 1 Kit
EUR 854.4

Goat Insulin ELISA Kit, 96 tests, Quantitative

10730 1 Kit
EUR 927.6

Human CXCL10 ELISA Kit, 96 tests, Quantitative

HCXC-010-100 1 kit
EUR 708

Human CXCL16 ELISA Kit, 96 tests, Quantitative

HCXC-016-100 1 kit
EUR 578.4

Benedicts Solution Quantitative

RRSP900-D 500ml
EUR 6.36

Benedicts Solution Quantitative

RRSP900-E 1L
EUR 10.59

Benedicts Solution Quantitative

RRSP900-F 2.5L
EUR 21.96

Rubella Virus IgG quantitative

GWB-31CB3E 1x96 Assays Ask for price

Deoxynivalenol/Vomitoxin Quantitative Test Kit

Q602 96T
EUR 200
Description: Feed Test

TBE / FSME IgG plus quantitative

GWB-3EBB47 1x96 Assays Ask for price

Bovine IL-4 ELISA Kit, 96 tests, Quantitative

BIL-004-100 1 kit
EUR 634.8

Human IL-10 ELISA Kit, 96 tests, Quantitative

HIL-010-120 1 kit
EUR 578.4

Human IL-13 ELISA Kit, 96 tests, Quantitative

HIL-013-130 1 kit
EUR 607.2

Human IL-15 ELISA Kit, 96 tests, Quantitative

HIL-015-135 1 kit
EUR 607.2

Human IL-21 ELISA Kit, 96 tests, Quantitative

HIL-021-145 1 kit
EUR 578.4

Human IL-22 ELISA Kit, 96 tests, Quantitative

HIL-022-150 1 kit
EUR 607.2

Human IL-23 ELISA Kit, 96 tests, Quantitative

HIL-023-155 1 kit
EUR 607.2

Human IL-27 ELISA Kit, 96 tests, Quantitative

HIL-027-160 1 kit
EUR 607.2

Human IL-29 ELISA Kit, 96 tests, Quantitative

HIL-029-165 1 kit
EUR 578.4

Mouse IL-10 ELISA Kit, 96 tests, Quantitative

MIL-010-125 1 kit
EUR 578.4

Mouse IL-13 ELISA Kit, 96 tests, Quantitative

MIL-013-135 1 kit
EUR 578.4

Mouse IL-21 ELISA Kit, 96 tests, Quantitative

MIL-021-155 1 kit
EUR 607.2

Mouse IL-22 ELISA Kit, 96 tests, Quantitative

MIL-022-160 1 kit
EUR 607.2

Mouse IL-23 ELISA Kit, 96 tests, Quantitative

MIL-023-165 1 kit
EUR 607.2

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