Molecular Blood Group Screening in Donors from Arabian Countries and Iran Using High-Throughput MALDI-TOF Mass Spectrometry and PCR-SSP
Background and aims: Only little is known about blood groups other than ABO blood groups and Rhesus factors in Arabian countries and Iran. During the last years, increased migration to Central Europe has put a focus on the question how to guarantee blood supply for patients from these countries, particularly because hemoglobinopathies with the need of regular blood support are more frequent in patients from that region. Therefore, blood group allele frequencies should be determined in individuals from Arabian countries and Iran by molecular typing and compared to a German rare donor panel.
Methods: 1,111 samples including 800 individuals from Syria, 147 from Iran, 123 from the Arabian Peninsula, and 41 from Northern African countries were included in a MALDI-TOF MS assay to detect polymorphisms coding for Kk, Fy(a/b), Fy<sub>null</sub>, C<sub>w</sub>, Jk(a/b), Jo(a+/a-), Lu(a/b), Lu(8/14), Ss, Do(a/b), Co(a/b), In(a/b), Js(a/b), Kp(a/b), and variant alleles RHCE*c.697C>G and RHCE *c.733C>G. Yt(a/b), S-s-U-, Vel<sub>null</sub>, Co<sub>null</sub>, and RHCE *c.667G>T were tested by PCR-SSP.
<strong class=”sub-title”>
Results: </strong> Of the Arabian donors, 2% were homozygous for the <i>FY</i> *<i>02.01N</i> allele (Fy<sub>null</sub>), and 15.7% carried the heterozygous mutation. However, 0.8% of the German donors also carried 1 copy of the allele. 3.6% of all and 29.3% of Northern African donors were heterozygous for the <i>RHCE</i> *<i>c.733C>G</i> substitution, 0.4% of the Syrian probands were heterozygous for <i>DO</i> *<i>01/DO</i> *<i>01.-05</i>, a genotype that was lacking in German donors.
 PCR kit) The egg drop syndrome (76) PCR kit |
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| PCR-V044-48D | Bioingentech | 50T | EUR 425.80 |
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Description: An conventional PCR kit for detection of The egg drop syndrome (76) |
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The egg drop syndrome (76) PCR kit |
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| PCR-V044-96D | Bioingentech | 100T | EUR 521.50 |
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Description: An conventional PCR kit for detection of The egg drop syndrome (76) |
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 PCR Mix |
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Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol. |
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Description: A Real-Time PCR kit for detection of Egg Drop Syndrome . |
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Egg Drop Syndrome RT PCR kit |
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Description: A Real-Time PCR kit for detection of Egg Drop Syndrome . |
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Description: A Real-Time PCR kit for detection of Egg Drop Syndrome . |
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Description: Disposable Pipets; Disposable Pipets, Serological, Misc |
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Description: Real Time PCR Kit is a screening assay for a rapid and accurate detection of Egg Drop Syndrome. |
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Egg Drop Syndrome One-Step PCR kit |
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| Oneq-V026-150D | Bioingentech | 150T | EUR 838.75 |
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Description: Real Time PCR Kit is a screening assay for a rapid and accurate detection of Egg Drop Syndrome. |
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Egg Drop Syndrome One-Step PCR kit |
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Description: Real Time PCR Kit is a screening assay for a rapid and accurate detection of Egg Drop Syndrome. |
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 7077B PIPET SHRTY 1ML |
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| 7077B-1 | CORNING | 250/pk | EUR 231.00 |
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Description: Disposable Pipets; Disposable Pipets, Serological, Misc |
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Description: Disposable Pipets; Disposable Pipets, Serological, Misc |
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 RT PCR kit) The egg drop syndrome (76) RT PCR kit |
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| RTq-V044-100D | Bioingentech | 100T | EUR 628.50 |
|
|||
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Description: A Real-Time PCR kit for detection of The egg drop syndrome (76) . |
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The egg drop syndrome (76) RT PCR kit |
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| RTq-V044-150D | Bioingentech | 150T | EUR 701.00 |
|
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Description: A Real-Time PCR kit for detection of The egg drop syndrome (76) . |
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The egg drop syndrome (76) RT PCR kit |
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| RTq-V044-50D | Bioingentech | 50T | EUR 532.80 |
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Description: A Real-Time PCR kit for detection of The egg drop syndrome (76) . |
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PRICE DROP |
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 One-Step PCR kit) The egg drop syndrome (76) One-Step PCR kit |
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| Oneq-V044-100D | Bioingentech | 100T | EUR 747.40 |
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Description: Real Time PCR Kit is a screening assay for a rapid and accurate detection of The egg drop syndrome (76). |
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The egg drop syndrome (76) One-Step PCR kit |
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| Oneq-V044-150D | Bioingentech | 150T | EUR 838.75 |
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Description: Real Time PCR Kit is a screening assay for a rapid and accurate detection of The egg drop syndrome (76). |
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The egg drop syndrome (76) One-Step PCR kit |
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| Oneq-V044-50D | Bioingentech | 50T | EUR 628.50 |
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Description: Real Time PCR Kit is a screening assay for a rapid and accurate detection of The egg drop syndrome (76). |
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 Taq 5x PCR Mix 12.5 mM |
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Description: High quality Taq polymerase for different PCR variations and downstream applications. |
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Description: Bioscience Mag Beads; Magnetic PCR Normaliser |
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Description: An conventional PCR kit for detection of Poliovirus 1 (PV1) |
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Poliovirus 1 (PV1) PCR kit |
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Description: An conventional PCR kit for detection of Poliovirus 1 (PV1) |
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Description: An conventional PCR kit for detection of Psittacid herpesvirus 1 |
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Psittacid herpesvirus 1 PCR kit |
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| PCR-V014-96D | Bioingentech | 100T | EUR 521.50 |
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Description: An conventional PCR kit for detection of Psittacid herpesvirus 1 |
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Description: An conventional PCR kit for detection of Equine herpesvirus 1 |
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Equine herpesvirus 1 PCR kit |
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| PCR-V144-96D | Bioingentech | 100T | EUR 521.50 |
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Description: An conventional PCR kit for detection of Equine herpesvirus 1 |
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Description: An conventional PCR kit for detection of Bovine herpesvirus 1 |
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Bovine herpesvirus 1 PCR kit |
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Description: An conventional PCR kit for detection of Bovine herpesvirus 1 |
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Description: An conventional PCR kit for detection of Bluetongue virus 1 |
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Bluetongue virus 1 PCR kit |
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Description: An conventional PCR kit for detection of Bluetongue virus 1 |
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Description: PCR Plates & Tubes; A354 PCR Plate-Axygen |
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| PCR-96-LC480-C-NF | CORNING | 10/pk | EUR 199.00 |
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Description: PCR Plates & Tubes; A354 PCR Plate-Axygen |
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) 96 WELL PCR MICROPLATE FOR ROCHE 480 LIGHT CYCLER, WHITE (NO SEALING FILM) |
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| PCR-96-LC480-W-NF | CORNING | 10/pk | EUR 199.00 |
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Description: PCR Plates & Tubes; A354 PCR Plate-Axygen |
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 One-Step BrightGreen qRT-PCR-No Dye |
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| G471-S | ABM | 100 rxn (20 ul/rxn) | EUR 158.00 |
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| BS9291 | BBI Biotech | 5X1mL, 5ml | EUR 209.50 |
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HotStart PCR Master mix (2X, Green Dye) |
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| BS9292 | BBI Biotech | 1ml | EUR 96.40 |
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Whereas the <i>KEL</i> *<i>02.06</i> allele coding for the Js(a) phenotype was missing in Germans; 0.8% of the Syrian donors carried 1 copy of this allele. 1.8% of the Syrian but only 0.3% of the German donors were negative for <i>YT</i> *<i>01</i>. One donor from Northern Africa homo-zygously carried the <i>GYPB</i> *<i>270+5g>t</i> mutation, inducing the S-s-U+<sup>w</sup> phenotype, and in 2 German donors a <i>GYPB</i> *<i>c.161G>A</i> exchange, which induces the Mit+ phenotype, caused a <i>GYPB</i> *<i>03</i> allele dropout in the MALDI assay. The overall failure rate of the Arabian panel was 0.4%.
CRISPR-mediated knock-in in the mouse embryo using long single stranded DNA donors synthesised by biotinylated PCR
Successful gene knock-in by CRISPR-Cas9 in the mouse zygote requires three components; guideRNA, Cas9 protein and a suitable donor template, which usually comprises homology flanked insert sequence. Recently, long single stranded DNA (lssDNA) donors have emerged as a popular choice of DNA donor, outperforming dsDNA templates in terms of knock-in efficiency for gene tagging and generating conditional alleles.
The generation of these donors can be achieved through several methods that may introduce errors in the sequence, result in poor yields, and contain dsDNA contamination. We have developed our own cost-effective lssDNA synthesis methodology that results in high purity, sequence verified, low contamination lssDNA donors. We provide a detailed methodology on the design and generation of such donors for gene tagging experiments and generating conditional alleles.
BDNF Val66Met Polymorphism, the Allele-Specific Analysis by qRT-PCR – a Novel Protocol
Background: Alteration in brain-derived neurotrophic factor (BDNF) production is a marker of neuropathological conditions, which has led to the investigation of Val66Met polymorphism occurring in the human BDNF gene (BDNF). Presently, there are no reported methods available for the analysis of Val66Met impact on human BDNF functioning.
Purpose: To develop a qRT-PCR protocol for the allele-specific expression evaluation of the Val66Met polymorphism in BDNF.
Methods: Using RNA extracted from muscle samples of 9 healthy volunteers (32.9 ± 10.3 y) at rest and following a maximal effort aerobic capacity exercise test, a protocol was developed for the detection of Val66/Met66 allele-specific BDNF expression in Real-Time Quantitative Reverse Transcription PCR (qRT-PCR) – relative to housekeeping genes – and validated by absolute quantification in Droplet Digital Polymerase Chain Reaction (ddPCR).
Results: Differences in the relative values of BDNF mRNA were confirmed by ddPCR analysis. HPRT1 and B2M were the most stable genes expressed in muscle tissue among different metabolic conditions, while GAPDH revealed to be metabolic responsive. Conclusion: Our qRT-PCR protocol successfully determines the allele-specific detection and changes in BDNF expression regarding the Val66Met polymorphism.
Standardization of BCR-ABL1 p210 Monitoring: From Nested to Digital PCR
The introduction of tyrosine kinase inhibitors in 2001 as a targeted anticancer therapy has significantly improved the quality of life and survival of patients with chronic myeloid leukemia. At the same time, with the introduction of tyrosine kinase inhibitors, the need for precise monitoring of the molecular response to therapy has emerged. Starting with a qualitative polymerase chain reaction, followed by the introduction of a quantitative polymerase chain reaction to determine the exact quantity of the transcript of interest-p210 BCR-ABL1, molecular monitoring in patients with chronic myeloid leukemia was internationally standardized.
This enabled precise monitoring of the therapeutic response, unification of therapeutic protocols, and comparison of results between different laboratories. This review aims to summarize the steps in the diagnosis and molecular monitoring of p210 BCR-ABL1, as well as to consider the possible future application of a more sophisticated method such as digital polymerase chain reaction.
Droplet Digital PCR Analysis of Liquid Biopsy Samples Unveils the Diagnostic Role of hsa-miR-133a-3p and hsa-miR-375-3p in Oral Cancer
Despite the availability of screening programs, oral cancer deaths are increasing due to the lack of diagnostic biomarkers leading to late diagnosis and a poor prognosis. Therefore, there is an urgent need to discover novel effective biomarkers for this tumor. On these bases, the aim of this study was to validate the diagnostic potential of microRNAs (miRNAs) through the analysis of liquid biopsy samples obtained from ten oral cancer patients and ten healthy controls. The expression of four selected miRNAs was evaluated by using droplet digital PCR (ddPCR) in a pilot cohort of ten oral cancer patients and ten healthy donors. Bioinformatics analyses were performed to assess the functional role of these miRNAs.
The expression levels of the predicted down-regulated hsa-miR-133a-3p and hsa-miR-375-3p were significantly reduced in oral cancer patients compared to normal individuals while no significant results were obtained for the up-regulated hsa-miR-503-5p and hsa-miR-196a-5p. ROC analysis confirmed the high sensitivity and specificity of hsa-miR-375-3p and hsa-miR-133a-3p. Therefore, both miRNAs are significantly down-regulated in cancer patients and can be used as biomarkers for the early diagnosis of oral cancer. The analysis of circulating miRNAs in a larger series of patients is mandatory to confirm the results obtained in this pilot study.
) SYBR Green qPCR Master Mix (High ROX) |
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| HY-K0521 | MedChemExpress | 1 mL (100 rxns) | EUR 113.00 |
) SYBR Green qPCR Master Mix (Low ROX) |
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| HY-K0522 | MedChemExpress | 5 mL (500 rxns ) | EUR 257.00 |
SYBR Green qPCR Master Mix (No ROX) |
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| HY-K0523 | MedChemExpress | 5 mL (500 rxns ) | EUR 257.00 |
 AceQ qPCR SYBR® Green Master Mix |
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| Q111-02 | Vazyme | 500 rxn (20 μl/rxn) | EUR 221.00 |
AceQ qPCR SYBR® Green Master Mix |
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| Q111-03 | Vazyme | 2500 rxn (20 μl/rxn) | EUR 646.00 |
) 2x SYBR Green qPCR Master Mix (High ROX) |
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| B21402 | Bimake | 5 ml | EUR 224.00 |
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Description: 2x SYBR Green qPCR master mix (High ROX) utilizes a special performance-enhanced Taq DNA polymerase protected via a hot-start activation technique, and optimized qPCR buffer system to perform SYBR |
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2x SYBR Green qPCR Master Mix (High ROX) |
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| B21403 | Bimake | 25 ml | EUR 856.00 |
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Description: 2x SYBR Green qPCR master mix (High ROX) utilizes a special performance-enhanced Taq DNA polymerase protected via a hot-start activation technique, and optimized qPCR buffer system to perform SYBR |
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) 2x SYBR Green qPCR Master Mix (Low ROX) |
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| B21702 | Bimake | 5 ml | EUR 224.00 |
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Description: 2x SYBR Green qPCR master mix (Low ROX) utilizes a special performance-enhanced Taq DNA polymerase protected via a hot-start activation technique, and optimized qPCR buffer system to perform SYBR |
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2x SYBR Green qPCR Master Mix (Low ROX) |
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| B21703 | Bimake | 25 ml | EUR 856.00 |
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Description: 2x SYBR Green qPCR master mix (Low ROX) utilizes a special performance-enhanced Taq DNA polymerase protected via a hot-start activation technique, and optimized qPCR buffer system to perform SYBR |
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 ig SYBR Green qPCR 2X Master Mix - 500 Reactions |
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| 3356 | Intact Genomics | 1/EA | EUR 288.00 |
 AceQ Universal SYBR qPCR Master Mix |
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| Q511-02 | Vazyme | 500 rxn (20 μl/rxn) | EUR 221.00 |
AceQ Universal SYBR qPCR Master Mix |
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| Q511-03 | Vazyme | 2500 rxn (20 μl/rxn) | EUR 646.00 |
 ChamQ Universal SYBR qPCR Master Mix |
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| Q711-02 | Vazyme | 500 rxn (20 μl/rxn) | EUR 221.00 |
ChamQ Universal SYBR qPCR Master Mix |
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| Q711-03 | Vazyme | 2500 rxn (20 μl/rxn) | EUR 646.00 |
 miRNA Universal SYBR® qPCR Master Mix |
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| MQ101-01 | Vazyme | 125 rxn(20 μl/rxn) | EUR 138.00 |
miRNA Universal SYBR® qPCR Master Mix |
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| MQ101-02 | Vazyme | 500 rxn(20 μl/rxn) | EUR 242.00 |
 RT Master Mix for qPCR |
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| HY-K0510 | MedChemExpress | 1 mL (100 rxns) | EUR 291.00 |
 AceQ qPCR Probe Master Mix |
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| Q112-02 | Vazyme | 500 rxn (20 μl/rxn) | EUR 221.00 |
AceQ qPCR Probe Master Mix |
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| Q112-03 | Vazyme | 2500 rxn (20 μl/rxn) | EUR 646.00 |
) amfiSure Ultra Fidelity PCR Master Mix(2X) |
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| P0346-001 | GenDepot | 1ml | EUR 280.00 |
amfiSure Ultra Fidelity PCR Master Mix(2X) |
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| P0346-002 | GenDepot | 2x1ml | EUR 482.00 |
amfiSure Ultra Fidelity PCR Master Mix(2X) |
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| P0346-005 | GenDepot | 5x1ml | EUR 828.00 |
amfiSure Ultra Fidelity PCR Master Mix(2X) |
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| P0346-010 | GenDepot | 10x1ml | EUR 1360.00 |
HotStart PCR Master mix (2X, Green Dye) |
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| BS9291 | BBI Biotech | 5X1mL, 5ml | EUR 209.50 |
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HotStart PCR Master mix (2X, Green Dye) |
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| BS9292 | BBI Biotech | 1ml | EUR 96.40 |
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 PLATEMAX ULTRA CLEAR PERMANENT HEAT SEALING FILM FOR QPCR, 100/500 |
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| HS-100-QPCR | CORNING | 100/pk | EUR 318.00 |
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Description: Sealing Products; Sealing films - Axygen |
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 PLATEMAX ULTRA CLEAR PEELABLE HEAT SEALING FILM FOR QPCR. 100/500 |
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| HS-150-QPCR | CORNING | 100/pk | EUR 318.00 |
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Description: Sealing Products; Sealing films - Axygen |
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) Fast EvaGreen Master Mix for qPCR(200 rxn) |
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| 31003 | Biotium | 2x1ML | EUR 204.00 |
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Description: Minimum order quantity: 1 unit of 2x1ML |
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) Fast EvaGreen Master Mix for qPCR (500 rxn) |
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| 31003-1 | Biotium | 5x1ML | EUR 386.00 |
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Description: Minimum order quantity: 1 unit of 5x1ML |
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) Fast EvaGreen Master Mix for qPCR (5000 rxn) |
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| 31003-2 | Biotium | 50x1ML | EUR 3116.00 |
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Description: Minimum order quantity: 1 unit of 50x1ML |
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) Fast Plus EvaGreen qPCR Master Mix (200 rxn) |
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| 31020 | Biotium | 2x1mL | EUR 228.00 |
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Description: Minimum order quantity: 1 unit of 2x1mL |
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) Fast Plus EvaGreen qPCR Master Mix (500 rxn) |
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| 31020-1 | Biotium | 5x1mL | EUR 437.00 |
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Description: Minimum order quantity: 1 unit of 5x1mL |
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) Fast Plus EvaGreen qPCR Master Mix (5000 rxn) |
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| 31020-2 | Biotium | 50x1mL | EUR 3576.00 |
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Description: Minimum order quantity: 1 unit of 50x1mL |
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) RT Master Mix for qPCR (gDNA digester plus) |
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| HY-K0511 | MedChemExpress | 1 mL (100 rxns) | EUR 315.00 |
 SYBR Green I |
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| B1747-25 | Biovision | EUR 1164.00 | |
SYBR Green I |
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| B1747-5 | Biovision | EUR 387.00 | |
) Forget-Me-Notâ„¢ EvaGreen qPCR Master Mix (500 reactions) |
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| 31041-1 | Biotium | 5x1mL | EUR 272.00 |
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Description: Minimum order quantity: 1 unit of 5x1mL |
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) Forget-Me-Notâ„¢ EvaGreen qPCR Master Mix (2000 reactions) |
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| 31041-20mL | Biotium | 2x10mL | EUR 494.00 |
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Description: Minimum order quantity: 1 unit of 2x10mL |
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) Forget-Me-Notâ„¢ EvaGreen qPCR Master Mix (100 reactions) |
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| 31041-T | Biotium | 1mL | EUR 114.00 |
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Description: Minimum order quantity: 1 unit of 1mL |
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 Green qPCR SuperMix |
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| 20-abx098031 | Abbexa |
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 EZSolution? SYBR Green I |
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| B1748-1 | Biovision | EUR 620.00 | |
EZSolution? SYBR Green I |
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| B1748-500 | Biovision | EUR 430.00 | |
 Jade? Master Mix |
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| M1105-500 | Biovision | EUR 441.00 | |
 Taqman Master Mix |
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| M1121-500 | Biovision | EUR 441.00 | |
) Fast EvaGreen Master Mix for qPCR, trial size (100 rxn) |
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| 31003-T | Biotium | 1ML | EUR 123.00 |
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Description: Minimum order quantity: 1 unit of 1ML |
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) Fast Plus EvaGreen qPCR Master Mix (trial size, 100 rxn) |
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| 31020-T | Biotium | 1mL | EUR 134.00 |
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Description: Minimum order quantity: 1 unit of 1mL |
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) OPTIONAL GREEN FILTER (572NM) |
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| GDBL-GREEN | CORNING | 1/pk | EUR 1052.00 |
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Description: Lab Equipment; Axygen Branded EQ |
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 Green Taq Mix |
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| P131-01 | Vazyme | 5 ml | EUR 119.00 |
Green Taq Mix |
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| P131-02 | Vazyme | 15 ml | EUR 150.00 |
Green Taq Mix |
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| P131-03 | Vazyme | 50 ml | EUR 248.00 |
 Green qPCR SuperMix UDG |
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| 20-abx098032 | Abbexa |
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 Top Green qPCR SuperMix |
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| 20-abx098033 | Abbexa |
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 Tip Green qPCR SuperMix |
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| 20-abx098034 | Abbexa |
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) Fast Plus EvaGreen Master Mix for qPCR with Rox (200 rxn) |
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| 31014 | Biotium | 2x1mL | EUR 228.00 |
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Description: Minimum order quantity: 1 unit of 2x1mL |
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) Fast Plus EvaGreen qPCR Master Mix with low Rox (500 rxn) |
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| 31014-1 | Biotium | 5x1mL | EUR 437.00 |
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Description: Minimum order quantity: 1 unit of 5x1mL |
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) Fast Plus EvaGreen qPCR Master Mix with low Rox (5000 rxn) |
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| 31014-2 | Biotium | 50x1mL | EUR 3576.00 |
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Description: Minimum order quantity: 1 unit of 50x1mL |
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) Fast Plus EvaGreen qPCR Master Mix with high Rox (500 rxn) |
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| 31015-1 | Biotium | 5x1mL | EUR 437.00 |
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Description: Minimum order quantity: 1 unit of 5x1mL |
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) Fast Plus EvaGreen qPCR Master Mix with high Rox (5000 rxn) |
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| 31015-2 | Biotium | 50x1mL | EUR 3576.00 |
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Description: Minimum order quantity: 1 unit of 50x1mL |
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) Forget-Me-Notâ„¢ EvaGreen qPCR Master Mix with ROX (500 reactions) |
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| 31042-1 | Biotium | 5x1mL | EUR 272.00 |
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Description: Minimum order quantity: 1 unit of 5x1mL |
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) Forget-Me-Notâ„¢ EvaGreen qPCR Master Mix with ROX (200 reactions) |
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| 31042-20mL | Biotium | 2x10mL | EUR 494.00 |
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Description: Minimum order quantity: 1 unit of 2x10mL |
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) Forget-Me-Notâ„¢ EvaGreen qPCR Master Mix with ROX (100 reactions) |
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| 31042-T | Biotium | 1mL | EUR 114.00 |
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Description: Minimum order quantity: 1 unit of 1mL |
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 (500 reactions)) Forget-Me-Notâ„¢ EvaGreen qPCR Master Mix (Low ROX) (500 reactions) |
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| 9-31045 | Biotium |
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Description: Minimum order quantity: 1 unit of 5x1mL |
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 (500 reactions)) Forget-Me-Notâ„¢ EvaGreen qPCR Master Mix (High ROX) (500 reactions) |
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| 9-31046 | Biotium |
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Description: Minimum order quantity: 1 unit of 5x1mL |
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, packed in 1,25 ml vials) qPCR Master Mix DLP1 (SafeLine: dUTP), packed in 1,25 ml vials |
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| S190 | GeneOn | 100 rcs (2,5 ml) | EUR 138.00 |
qPCR Master Mix DLP1 (SafeLine: dUTP), packed in 1,25 ml vials |
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| S190L | GeneOn | 500 rcs (12,5 ml) | EUR 523.00 |
 2 × AceTaq Master Mix |
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| P411-01 | Vazyme | 1 ml | EUR 119.00 |
2 × AceTaq Master Mix |
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| P411-02 | Vazyme | 5 ml | EUR 158.00 |
2 × AceTaq Master Mix |
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| P411-03 | Vazyme | 15 ml | EUR 262.00 |
amfiSure PCR Master Mix |
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| P0311-010 | GenDepot | 2x50 rxns | EUR 126.00 |
amfiSure PCR Master Mix |
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| P0311-025 | GenDepot | 5X50 rxns | EUR 165.00 |
amfiSure PCR Master Mix |
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| P0311-050 | GenDepot | 10X50 rxns | EUR 219.00 |
amfiSure PCR Master Mix |
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| P0311-100 | GenDepot | 10x1ml | EUR 286.00 |
amfiSure PCR Master Mix |
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| P0311-125 | GenDepot | 15X50 rxns | EUR 278.00 |
amfiSure PCR Master Mix |
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| P0311-200 | GenDepot | 20x1ml | EUR 469.00 |
amfiSure PCR Master Mix |
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| P0311-250 | GenDepot | 50x50 rxns | EUR 752.00 |
amfiSure PCR Master Mix |
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| P0311-500 | GenDepot | 100x50 rxns | EUR 1258.00 |
amfiXpand PCR Master Mix |
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| P0331-010 | GenDepot | 2X50 rxns | EUR 151.00 |
amfiXpand PCR Master Mix |
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| P0331-025 | GenDepot | 5X50 rxns | EUR 273.00 |
amfiXpand PCR Master Mix |
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| P0331-050 | GenDepot | 10X50 rxns | EUR 448.00 |
amfiXpand PCR Master Mix |
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| P0331-250 | GenDepot | 50x50 rxns | EUR 1873.00 |
 2 × Taq Master Mix |
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| P111-01 | Vazyme | 5 ml | EUR 117.00 |
2 × Taq Master Mix |
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| P111-02 | Vazyme | 15 ml | EUR 146.00 |
2 × Taq Master Mix |
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| P111-03 | Vazyme | 50 ml | EUR 236.00 |
 Taqman Master Mix-iCycler |
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| M1122-500 | Biovision | EUR 441.00 | |
 Taqman Master Mix-ROX |
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| M1124-500 | Biovision | EUR 441.00 | |
 Taqman Master Mix-Multiplex |
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| M1125-500 | Biovision | EUR 441.00 | |
 Accuris Taq Master Mix |
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| PR1001-1000 | BenchMark | 1 PC | EUR 300.26 |
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Accuris Taq Master Mix |
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| PR1001-200 | BenchMark | 1 PC | EUR 121.62 |
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Accuris Taq Master Mix |
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| PR1001-S | BenchMark | 1 PC | EUR 69.79 |
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 20 profiles) LncRNA Profiler Complete qPCR Array Kit (cDNA synthesis kit, qPCR array and SYBR Green reagent) 20 profiles |
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| RA910A-1 | SBI | 20 profiles | EUR 1108.00 |
|
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: (1mL)) Fast Plus EvaGreen qPCR Master Mix with low Rox (100 rxn): (1mL) |
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| 31014-T | Biotium | 1mL | EUR 134.00 |
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Description: Minimum order quantity: 1 unit of 1mL |
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) Fast Plus EvaGreen Master Mix for qPCR with High Rox (200 rxn) |
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| 31015 | Biotium | 2x1mL | EUR 228.00 |
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Description: Minimum order quantity: 1 unit of 2x1mL |
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: (1mL)) Fast Plus EvaGreen qPCR Master Mix with High Rox (100 rxn): (1mL) |
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| 31015-T | Biotium | 1mL | EUR 134.00 |
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Description: Minimum order quantity: 1 unit of 1mL |
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, packed in 1,25 ml vials) qPCR Master Mix E1 (SafeLine: EvaGreen, dUTP), packed in 1,25 ml vials |
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| S150 | GeneOn | 100 rcs (2,5 ml) | EUR 138.00 |
qPCR Master Mix E1 (SafeLine: EvaGreen, dUTP), packed in 1,25 ml vials |
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| S150L | GeneOn | 500 rcs (12,5 ml) | EUR 523.00 |
