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A Novel Multiplex RT-PCR Assay for Simultaneous Detection of Dengue and Chikungunya Viruses

by Preston | Posted on

GLAD-PCR Assay of R(5mC)GY Sites in the Regulatory Region of Tumor-Suppressor Genes Associated with Gastric Cancer

 

At early stages of carcinogenesis, the regulatory regions of some tumor suppressor genes become aberrantly methylated at RCGY sites, which are substrates of DNA methyltransferase Dnmt3. Identification of aberrantly methylated sites in tumor DNA is considered to be the first step in the development of epigenetic PCR test systems for early diagnosis of cancer. Recently, we have developed a GLAD-PCR assay, a method for detecting the R(5mC)GY site in the genome position of interest even at significant excess of DNA molecules with a non-methylated RCGY site in this location.

 

The aim of the present work is to use the GLAD-PCR assay to detect the aberrantly methylated R(5mC)GY sites in the regulatory regions of tumor suppressor genes (brinp1, bves, cacna2d3, cdh11, cpeb1, epha7, fgf2, galr1, gata4, hopx, hs3st2, irx1, lrrc3b, pcdh10, rprm, runx3, sfrp2, sox17, tcf21, tfpi2, wnt5a, zfp82, and znf331) in DNA samples obtained from gastric cancer (GC) tissues. The study of the DNA samples derived from 29 tumor and 25 normal gastric tissue samples demonstrated a high diagnostic potential of the selected RCGY sites in the regulatory regions of the irx1, cacna2d3, and epha7 genes; the total indices of sensitivity and specificity for GC detection being 96.6% and 100%, respectively.

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Ureaplasma parvum RT PCR kit
RTq-H458-150D 150T
EUR 701
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Description: A Real-Time PCR kit for detection of Ureaplasma parvum .

Ureaplasma parvum RT PCR kit
RTq-H458-50D 50T
EUR 532.8
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Description: A Real-Time PCR kit for detection of Ureaplasma parvum .

MDV Real-Time PCR Kit
PD65-06 96t
EUR 1553.6
Description: Please check the datasheet of MDV Real-Time PCR Kit before using the test.

ALV Real-Time PCR Kit
PD65-15 96t
EUR 1724.4
Description: Please check the datasheet of ALV Real-Time PCR Kit before using the test.

Ureaplasma parvum One-Step PCR kit
Oneq-H458-100D 100T
EUR 747.4
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Description: Real Time PCR Kit is a screening assay for a rapid and accurate detection of Ureaplasma parvum.

Ureaplasma parvum One-Step PCR kit
Oneq-H458-150D 150T
EUR 838.75
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Description: Real Time PCR Kit is a screening assay for a rapid and accurate detection of Ureaplasma parvum.

Ureaplasma parvum One-Step PCR kit
Oneq-H458-50D 50T
EUR 628.5
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Description: Real Time PCR Kit is a screening assay for a rapid and accurate detection of Ureaplasma parvum.

Cryptosporidium parvum PCR kit
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EUR 425.8
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Description: An conventional PCR kit for detection of Cryptosporidium parvum

Cryptosporidium parvum PCR kit
PCR-H486-96D 100T
EUR 521.5
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Description: An conventional PCR kit for detection of Cryptosporidium parvum

0.2ML CLEAR STRIP CAPS FOR REAL TIME PCR
PCR-2CP-RT-C 125/pk
EUR 223
Description: PCR Plates & Tubes; PCR Strip Tubes and Strip Caps - Axygen

Ureaplasma sp PCR kit
PCR-H459-48D 50T
EUR 425.8
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Description: An conventional PCR kit for detection of Ureaplasma sp

Ureaplasma sp PCR kit
PCR-H459-96D 100T
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Description: An conventional PCR kit for detection of Ureaplasma sp

Ureaplasma urealyticum PCR kit
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EUR 425.8
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Ureaplasma urealyticum PCR kit
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Ureaplasma Urealyticum Parvum Protein
20-abx260307
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hsa-mir-521 Real-time RT-PCR Detection Kit
20-abx097600
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dsGreen for Real-Time PCR, 100×, 50 mL
91010 50 mL
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81010 10 mL
EUR 869

dsGreen for Real-Time PCR, 100×, 5 mL
71010 5 mL
EUR 625

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51010 1.5 mL
EUR 331

dsGreen for Real-Time PCR, 100×, 1 mL
41010 1 mL
EUR 249

dsGreen for Real-Time PCR, 100×, 100 uL
11010 100 uL
EUR 59

dsGreen for Real-Time PCR, 100×, 2 mL
61010 2 mL
EUR 363

PMA Real-Time PCR Bacterial Viability Kit - Salmonella enterica (invA)
31033 1kit
EUR 428
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PMA Real-Time PCR Bacterial Viability Kit - Mycobacterium tuberculosis (groEL2)
31034 1kit
EUR 399
Description: Minimum order quantity: 1 unit of 1kit

PMA Real-Time PCR Bacterial Viability Kit - Staphylococcus aureus (nuc)
31035 1kit
EUR 399
Description: Minimum order quantity: 1 unit of 1kit

PMA Real-Time PCR Bacterial Viability Kit - Staphylococcus aureus (mecA)
31036 1kit
EUR 399
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PMA Real-Time PCR Bacterial Viability Kit - E. coli (uidA)
31050 1kit
EUR 428
Description: Minimum order quantity: 1 unit of 1kit

PMA Real-Time PCR Bacterial Viability Kit - Listeria monocytogenes (hly)
31051 1kit
EUR 399
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PMA Real-Time PCR Bacterial Viability Kit - Legionella pneumophila (mip)
31053 1kit
EUR 428
Description: Minimum order quantity: 1 unit of 1kit

Cryptosporidium parvum RT PCR kit
RTq-H486-100D 100T
EUR 628.5
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Description: A Real-Time PCR kit for detection of Cryptosporidium parvum .

Cryptosporidium parvum RT PCR kit
RTq-H486-150D 150T
EUR 701
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Description: A Real-Time PCR kit for detection of Cryptosporidium parvum .

Cryptosporidium parvum RT PCR kit
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EUR 532.8
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Ureaplasma parvum multi-banded antigen Antibody
abx022297-1mg 1 mg
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Ureaplasma parvum multi-banded antigen Antibody
abx022298-1mg 1 mg
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Ureaplasma parvum multi-banded antigen Antibody
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PMA Real-Time PCR Bacterial Viability Kit - Salmonella enterica (invA) PMAxx
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EUR 428
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PMA Real-Time PCR Bacterial Viability Kit - E. coli (uidA) PMAxx
31050-X 1kit
EUR 428
Description: Minimum order quantity: 1 unit of 1kit

PMA Real-Time PCR Bacterial Viability Kit - Listeria monocytogenes (hly) PMAxx
31051-X 1kit
EUR 428
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Novel Coronavirus COVID-19 (2019-nCoV) Real Time RT-PCR Kit
RR-0478-02 25 tests/kit
EUR 991
  • For use with PE5700, MJ-Opticon & other single color systems, ABI7000, ABI7300, ABI7500, ABI7900, ABI StepOne, StepOne plus, MJ-Opticon2, MJ-chromo4, MX3000P, MX3005P, Smart Cycler II, Rotor-Gene 6000, LightCycler 480, CFX 96, Life 96, Slan 96, iCycl
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Description: Novel Coronavirus (2019-nCoV) Real Time RT-PCR Kit is used for the qualitative detection of a novel coronavirus, which was identified in 2019 at Wuhan City, Hubei Province, China, in upper respiratory tract specimens (nasopharyngeal extracts, deep cough sputum, etc.) and lower respiratory tract specimens (alveoli irrigation fluid, etc.) by real time PCR systems.

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hsa-mir-521 Real-Time RT-PCR Detection and U6 Calibration Kit
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PMA Real-Time PCR Bacterial Viability Kit - E. coli O157:H7 (Z3276)
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Human Real-time PCR DNA Quantitation after QPCR DNA Damage Analysis Kit
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Recombinant Ureaplasma parvum MB (serovar 1) [His]
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EUR 637

PCR Mycoplasma Detection Kit
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Cryptosporidium parvum One-Step PCR kit
Oneq-H486-100D 100T
EUR 747.4
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Cryptosporidium parvum One-Step PCR kit
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Cryptosporidium parvum One-Step PCR kit
Oneq-H486-50D 50T
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Human Lung Cancer PCR Primer Library
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Human Schwann Cell PCR Primer Library
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qPCR plate-foil universal (for Real-time PCR, Light Cycler 480)
A26979 1x100 pcs
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TuMinute PCR Clean-Up Kit
P-1005 100 Samples
EUR 253
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Eztime Real - time PCRPremix 2x,Taqman
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AXYPREP MAG PCR CLEAN UP KIT- 1 ML - (SAMPLE SIZE)
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AXYPREP MAG PCR CLEAN UP KIT- 5 ML - 110 PREPS
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EUR 146
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AXYPREP MAG PCR CLEAN UP KIT- 50 ML - 1110 PREPS
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Ureaplasma sp RT PCR kit
RTq-H459-100D 100T
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Ureaplasma sp RT PCR kit
RTq-H459-150D 150T
EUR 701
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Description: A Real-Time PCR kit for detection of Ureaplasma sp .

Ureaplasma sp RT PCR kit
RTq-H459-50D 50T
EUR 532.8
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Description: A Real-Time PCR kit for detection of Ureaplasma sp .

Ureaplasma urealyticum RT PCR kit
RTq-H460-100D 100T
EUR 628.5
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Description: A Real-Time PCR kit for detection of Ureaplasma urealyticum .

Ureaplasma urealyticum RT PCR kit
RTq-H460-150D 150T
EUR 701
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Description: A Real-Time PCR kit for detection of Ureaplasma urealyticum .

Ureaplasma urealyticum RT PCR kit
RTq-H460-50D 50T
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Description: A Real-Time PCR kit for detection of Ureaplasma urealyticum .

PMA Real-Time PCR Bacterial Viability Kit - E. coli O157:H7 (Z3276) PMAxx
31037-X 1kit
EUR 428
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Eztime Real - time PCRPremix 2x,SYBR Green
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Eztime Real - time PCRPremix 2x,Taqman, ROX
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MicroElute PCR Clean Up Kit (50prep)
FAMPK-001 50 preps
EUR 126

MicroElute PCR Clean Up Kit (200prep)
FAMPK-001-1 200 preps
EUR 187

PCR Clean-Up Mini Kit (50prep)
FAPCK-001 50 preps
EUR 120

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FAPCK-001-1 200 preps
EUR 169

PCR Clean-Up Mini Kit (300prep)
FAPCK-001-2 300 preps
EUR 189

0,1ML THIN WALL TUBES 8 PER STRIP. CLEAR & REAL TIME STRIP CAPS
PCR-0108-LP-RT-C 125/pk
EUR 709
Description: PCR Plates & Tubes; PCR Strip Tubes and Strip Caps - Axygen

0,1ML THIN WALL TUBES 8 PER STRIP. WHITE & REAL TIME STRIP CAPS
PCR-0108-LP-RT-W 125/pk
EUR 770
Description: PCR Plates & Tubes; PCR Strip Tubes and Strip Caps - Axygen

Green Dye One Step qRT-PCR Master Mix For Quantitative Real Time PCR With ROX Dye
MB1004-100Reactions 100 Reactions
EUR 327

Ureaplasma sp One-Step PCR kit
Oneq-H459-100D 100T
EUR 747.4
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Ureaplasma sp One-Step PCR kit
Oneq-H459-150D 150T
EUR 838.75
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Ureaplasma sp One-Step PCR kit
Oneq-H459-50D 50T
EUR 628.5
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Ureaplasma urealyticum One-Step PCR kit
Oneq-H460-100D 100T
EUR 747.4
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Description: Real Time PCR Kit is a screening assay for a rapid and accurate detection of Ureaplasma urealyticum.

Ureaplasma urealyticum One-Step PCR kit
Oneq-H460-150D 150T
EUR 838.75
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Description: Real Time PCR Kit is a screening assay for a rapid and accurate detection of Ureaplasma urealyticum.

Ureaplasma urealyticum One-Step PCR kit
Oneq-H460-50D 50T
EUR 628.5
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Description: Real Time PCR Kit is a screening assay for a rapid and accurate detection of Ureaplasma urealyticum.

hsa-mir-34a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097657
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hsa-mir-34b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097659
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hsa-mir-92a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097661
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hsa-mir-92b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097662
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hsa-mir-93 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097663
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  • EUR 523.00
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hsa-mir-95 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097664
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  • EUR 523.00
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hsa-mir-96 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097665
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hsa-mir-98 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097666
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hsa-mir-99a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097667
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hsa-mir-99b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097669
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hsa-mir-100 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097671
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hsa-mir-101 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097673
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hsa-mir-103 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097675
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hsa-mir-105 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097676
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hsa-mir-106a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097677
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  • 100 rxns
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hsa-mir-106b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097678
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
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  • 50 rxns
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hsa-mir-107 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097679
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
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  • 50 rxns
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hsa-mir-124 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097680
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
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  • 50 rxns
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hsa-mir-125a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097681
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
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  • 50 rxns
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hsa-mir-127 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097686
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
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  • 50 rxns
  • Shipped within 3 weeks.

hsa-mir-129 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097689
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
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  • 50 rxns
  • Shipped within 3 weeks.

hsa-mir-130a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097690
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
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  • 50 rxns
  • Shipped within 3 weeks.

hsa-mir-130b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097691
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
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  • 50 rxns
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hsa-mir-132 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097692
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
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  • 50 rxns
  • Shipped within 3 weeks.

hsa-mir-133a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097693
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
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hsa-mir-133b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097694
  • EUR 732.00
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  • EUR 523.00
  • 100 rxns
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  • 50 rxns
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hsa-mir-134 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097695
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
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  • 50 rxns
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hsa-mir-135b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097696
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  • EUR 523.00
  • 100 rxns
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  • 50 rxns
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hsa-mir-137 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097699
  • EUR 732.00
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  • EUR 523.00
  • 100 rxns
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  • 50 rxns
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hsa-mir-138 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097700
  • EUR 732.00
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  • EUR 523.00
  • 100 rxns
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  • 50 rxns
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hsa-mir-139 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097701
  • EUR 732.00
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  • EUR 523.00
  • 100 rxns
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  • 50 rxns
  • Shipped within 3 weeks.

hsa-mir-140 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097702
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
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  • 50 rxns
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hsa-mir-141 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097703
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns
  • Shipped within 3 weeks.

hsa-mir-143 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097706
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns
  • Shipped within 3 weeks.

hsa-mir-145 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097708
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns
  • Shipped within 3 weeks.

hsa-mir-146a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097709
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns
  • Shipped within 3 weeks.

hsa-mir-149 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097711
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns
  • Shipped within 3 weeks.

hsa-mir-150 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097712
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns
  • Shipped within 3 weeks.

hsa-mir-151 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097714
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns
  • Shipped within 3 weeks.

hsa-mir-152 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097715
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns
  • Shipped within 3 weeks.

hsa-mir-153 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097716
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns
  • Shipped within 3 weeks.

hsa-mir-154 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097717
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns
  • Shipped within 3 weeks.

hsa-mir-181a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097719
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns
  • Shipped within 3 weeks.

hsa-mir-181b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097720
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns
  • Shipped within 3 weeks.

hsa-mir-181c Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097721
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns
  • Shipped within 3 weeks.

hsa-mir-181d Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097722
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns
  • Shipped within 3 weeks.

hsa-mir-182 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097723
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns
  • Shipped within 3 weeks.

hsa-mir-183 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097724
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns
  • Shipped within 3 weeks.

hsa-mir-184 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097725
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns
  • Shipped within 3 weeks.

hsa-mir-185 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097726
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns
  • Shipped within 3 weeks.

hsa-mir-190a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097728
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns
  • Shipped within 3 weeks.

A Novel Multiplex RT-PCR Assay for Simultaneous Detection of Dengue and Chikungunya Viruses

 

The goal of the study was to develop a specific, sensitive, and cost-effective molecular RT-PCR diagnostic assay for the rapid and simultaneous detection of the serotypes of dengue virus (DENV) and Chikungunya virus (CHIKV) from sera of suspected febrile patients. A single-tube, single-step multiplex RT-PCR (mRT-PCR) assay was designed for the detection of viral genomes from clinical and field samples.

 

Specificity and sensitivity of the mRT-PCR assay were evaluated against six different combinations using two reverse transcriptases (AMV-RT and RT-Ace) and three DNA polymerases (LA-Taq, rTaq, and Tth). Among the six combinations, the AMV-RT and LA-Taq combination was more specific and sensitive than other enzyme combinations for detecting viral genomes of DENV-1, DENV-2, DENV-3, and DENV-4 (p < 0.01), and for detecting viral genomes of CHIKV (p < 0.05). The detection limits of the mRT-PCR were 10 focus forming units (FFU) for CHIKV and 1 FFU, 20 FFU, 0.1 FFU, and 10 FFU for DENV-1, DENV-2, DENV-3, and DENV-4, respectively.

 

The primers used for the mRT-PCR did not show any cross-reactivity among the serotypes of DENV or CHIKV. Specificity and sensitivity of the newly developed mRT-PCR were validated using serum samples collected from febrile patients during dengue outbreaks in Bangladesh. The sensitivity for serotype detection of DENV and CHIKV was superior to the virus isolation method and the antigen detection method using the Dengue NS1-Ag assay. This novel mRT-PCR method can be used for molecular epidemiological surveillance of DENV and CHIKV in epidemic and endemic countries.

 

Development and evaluation of duplex TaqMan real-time PCR assay for detection and differentiation of wide-type and MGF505-2R gene-deleted African swine fever viruses

 

Background: African swine fever (ASF) is the most important disease to the pigs and cause serious economic losses to the countries with large-scale swine production. Vaccines are recognized as the most useful tool to prevent and control ASF virus (ASFV) infection. Currently, the MGF505 and MGF360 gene-deleted ASFVs or combined with CD2v deletion were confirmed to be the most promising vaccine candidates. Thus, it is essential to develop a diagnosis method to discriminate wide-type strain from the vaccines used.
Results: In this study, we established a duplex TaqMan real-time PCR based on the B646L gene and MGF505-2R gene. The sequence alignment showed that the targeted regions of primers and probes are highly conserved in the genotype II ASFVs. The duplex real-time assay can specifically detect B646L and MGF505-2R gene single or simultaneously without cross-reaction with other porcine viruses tested. The limit of detection was 5.8 copies and 3.0 copies for the standard plasmids containing B646L and MGF505-2R genes, respectively. Clinical samples were tested in parallel by duplex real-time PCR and a commercial ASFV detection kit. The detection results of these two assays against B646L gene were well consistent.
Conclusion: We successfully developed and evaluated a duplex TaqMan real-time PCR method which can effectively distinguish the wide type and MGF505 gene-deleted ASFVs. It would be a useful tool for the clinical diagnosis and control of ASF.

Gene Expression Analysis by Real-Time PCR in Nasal Brushings of Adult Patients with Allergic Rhinitis, Suspected Allergic Rhinitis, and Nonallergic Rhinitis

 

Background: Epithelial gene expression in allergic rhinitis patients has been evaluated by microarray. However, gene expression in patients with nonallergic rhinitis and suspected allergic rhinitis who reported allergen-related nasal symptoms but presented a negative atopic test was unknown.
Objectives: The aim of this study was to observe and compare epithelial gene expression in patients with allergic rhinitis, suspected allergic rhinitis, and nonallergic rhinitis.
Methods: Nasal brushings were collected from healthy controls and from patients with allergic rhinitis, suspected allergic rhinitis, and nonallergic rhinitis. The expressions of 20 genes selected from a previous microarray study were measured by real-time PCR. Associations of these genes with allergen type, disease duration and severity, the grade of nasal smear eosinophilia, and serum total IgE were analyzed.
Results: Twelve genes were confirmed to be upregulated in current adult allergic rhinitis patients allergic to multiple allergens, and 10 of them were also increased in the suspected allergic rhinitis and nonallergic rhinitis groups. TFF3 and ITLN1 expressions were increased in allergic rhinitis and suspected allergic rhinitis, but not nonallergic rhinitis. Different expressions between the allergic rhinitis and nonallergic rhinitis groups were found for 3 genes: CST1, TFF3, and ITLN1. In the allergic rhinitis patients, all 12 genes were upregulated in the seasonal and perennial groups; 9 of these 12 genes were also upregulated in the mixed group. In suspected allergic rhinitis patients, all 12 genes were upregulated in the perennial group; 8 of these 12 genes were also upregulated in the seasonal group and only 5 in the mixed group. No gene expression was associated with disease duration and serum total IgE. GCNT3 was positively correlated with the grade of nasal smear eosinophilia in the suspected allergic rhinitis group. Different genes were found to be associated with disease severity in different rhinitis groups.
Conclusions: Patients with allergic rhinitis, suspected allergic rhinitis, and nonallergic rhinitis showed much similarity with regard to epithelial gene expression; most genes were related to Th2 inflammation. CST1, TFF3, and ITLN1 might have the ability to differentiate allergic rhinitis from nonallergic rhinitis. Understanding the mechanisms underlying different types of rhinitis may be helpful for rhinitis diagnosis and treatment.

Application of Short Pre-enrichment, and Double Chemistry Real-Time PCR, Combining Fluorescent Probes and an Intercalating Dye, for Same-Day Detection and Confirmation of Salmonella spp. and Escherichia coli O157 in Ground Beef and Chicken Samples

 

 

Molecular methods, particularly those based on real-time PCR (qPCR), have become a popular approach to detect pathogens in food samples. This technique may take advantage of hydrolysis fluorescent probes for increased specificity. Even though suitable, this approach loses the capacity of performing result confirmation by melt curve analysis.

 

 

In the current study, we developed an alternative approach, combining fluorescent probes along with an intercalating dye (SYBR Green) in order to simultaneously detect, and confirm the result, of two foodborne pathogens (Salmonella spp. and Escherichia coli O157). This new approach named double chemistry qPCR was combined with a short pre-enrichment in order to obtain a multiplex “same-day” detection method for the selected pathogens. The evaluation of the novel method in spiked food samples (ground beef and chicken breast) obtained values of relative sensitivity, specificity, and accuracy higher than 95%, and Cohen’s kappa of 0.92, with a Limit of

 

Detection95 below 5 cfu/25 g, demonstrating its reliability. In addition to this, the method was challenged by inoculating heat-stressed bacteria as well as dead ones. It was observed that it was also possible to detect stressed bacteria with an initial inoculation level below 10 cfu/25 g. Also, it was noticed that high initial concentration of either pathogen (higher than 104 cfu/25 g) was needed in order to generate false positive results due to the presence of dead bacteria, thus the method presents potential for its application in the specific detection of live microorganisms.

 

ALV Real-Time PCR Kit
PD65-15 96t
EUR 1724.4
Description: Please check the datasheet of ALV Real-Time PCR Kit before using the test.

0.2ML CLEAR STRIP CAPS FOR REAL TIME PCR
PCR-2CP-RT-C 125/pk
EUR 223
Description: PCR Plates & Tubes; PCR Strip Tubes and Strip Caps - Axygen

Neisseria gonorrhoeae RT PCR kit
RTq-H450-100D 100T
EUR 628.5
  • Contact us in order to know the reactivity of the kit.
Description: A Real-Time PCR kit for detection of Neisseria gonorrhoeae .

Neisseria gonorrhoeae RT PCR kit
RTq-H450-150D 150T
EUR 701
  • Contact us in order to know the reactivity of the kit.
Description: A Real-Time PCR kit for detection of Neisseria gonorrhoeae .

Neisseria gonorrhoeae RT PCR kit
RTq-H450-50D 50T
EUR 532.8
  • Contact us in order to know the reactivity of the kit.
Description: A Real-Time PCR kit for detection of Neisseria gonorrhoeae .

Neisseria meningitidis PCR kit
PCR-H421-48D 50T
EUR 425.8
  • Contact us in order to know the reactivity of the kit.
Description: An conventional PCR kit for detection of Neisseria meningitidis

Neisseria meningitidis PCR kit
PCR-H421-96D 100T
EUR 521.5
  • Contact us in order to know the reactivity of the kit.
Description: An conventional PCR kit for detection of Neisseria meningitidis

Neisseria sp. PCR kit
PCR-H422-48D 50T
EUR 425.8
  • Contact us in order to know the reactivity of the kit.
Description: An conventional PCR kit for detection of Neisseria sp.

Neisseria sp. PCR kit
PCR-H422-96D 100T
EUR 521.5
  • Contact us in order to know the reactivity of the kit.
Description: An conventional PCR kit for detection of Neisseria sp.

Neisseria gonorrhoeae One-Step PCR kit
Oneq-H450-100D 100T
EUR 747.4
  • Contact us in order to know the reactivity of the kit.
Description: Real Time PCR Kit is a screening assay for a rapid and accurate detection of Neisseria gonorrhoeae.

Neisseria gonorrhoeae One-Step PCR kit
Oneq-H450-150D 150T
EUR 838.75
  • Contact us in order to know the reactivity of the kit.
Description: Real Time PCR Kit is a screening assay for a rapid and accurate detection of Neisseria gonorrhoeae.

Neisseria gonorrhoeae One-Step PCR kit
Oneq-H450-50D 50T
EUR 628.5
  • Contact us in order to know the reactivity of the kit.
Description: Real Time PCR Kit is a screening assay for a rapid and accurate detection of Neisseria gonorrhoeae.

hsa-mir-521 Real-time RT-PCR Detection Kit
20-abx097600
  • EUR 551.00
  • EUR 787.00
  • EUR 398.00
  • 100 rxns
  • 200 rxns
  • 50 rxns
  • Shipped within 3 weeks.

dsGreen for Real-Time PCR, 100×, 50 mL
91010 50 mL
EUR 3169

dsGreen for Real-Time PCR, 100×, 10 mL
81010 10 mL
EUR 869

dsGreen for Real-Time PCR, 100×, 5 mL
71010 5 mL
EUR 625

dsGreen for Real-Time PCR, 100×, 1.5 mL
51010 1.5 mL
EUR 331

dsGreen for Real-Time PCR, 100×, 1 mL
41010 1 mL
EUR 249

dsGreen for Real-Time PCR, 100×, 100 uL
11010 100 uL
EUR 59

dsGreen for Real-Time PCR, 100×, 2 mL
61010 2 mL
EUR 363

PMA Real-Time PCR Bacterial Viability Kit - Salmonella enterica (invA)
31033 1kit
EUR 428
Description: Minimum order quantity: 1 unit of 1kit

PMA Real-Time PCR Bacterial Viability Kit - Mycobacterium tuberculosis (groEL2)
31034 1kit
EUR 399
Description: Minimum order quantity: 1 unit of 1kit

PMA Real-Time PCR Bacterial Viability Kit - Staphylococcus aureus (nuc)
31035 1kit
EUR 399
Description: Minimum order quantity: 1 unit of 1kit

PMA Real-Time PCR Bacterial Viability Kit - Staphylococcus aureus (mecA)
31036 1kit
EUR 399
Description: Minimum order quantity: 1 unit of 1kit

PMA Real-Time PCR Bacterial Viability Kit - E. coli (uidA)
31050 1kit
EUR 428
Description: Minimum order quantity: 1 unit of 1kit

PMA Real-Time PCR Bacterial Viability Kit - Listeria monocytogenes (hly)
31051 1kit
EUR 399
Description: Minimum order quantity: 1 unit of 1kit

PMA Real-Time PCR Bacterial Viability Kit - Legionella pneumophila (mip)
31053 1kit
EUR 428
Description: Minimum order quantity: 1 unit of 1kit

PMA Real-Time PCR Bacterial Viability Kit - Salmonella enterica (invA) PMAxx
31033-X 1kit
EUR 428
Description: Minimum order quantity: 1 unit of 1kit

PMA Real-Time PCR Bacterial Viability Kit - E. coli (uidA) PMAxx
31050-X 1kit
EUR 428
Description: Minimum order quantity: 1 unit of 1kit

PMA Real-Time PCR Bacterial Viability Kit - Listeria monocytogenes (hly) PMAxx
31051-X 1kit
EUR 428
Description: Minimum order quantity: 1 unit of 1kit

Novel Coronavirus COVID-19 (2019-nCoV) Real Time RT-PCR Kit
RR-0478-02 25 tests/kit
EUR 991
  • For use with PE5700, MJ-Opticon & other single color systems, ABI7000, ABI7300, ABI7500, ABI7900, ABI StepOne, StepOne plus, MJ-Opticon2, MJ-chromo4, MX3000P, MX3005P, Smart Cycler II, Rotor-Gene 6000, LightCycler 480, CFX 96, Life 96, Slan 96, iCycl
  • Show more
Description: Novel Coronavirus (2019-nCoV) Real Time RT-PCR Kit is used for the qualitative detection of a novel coronavirus, which was identified in 2019 at Wuhan City, Hubei Province, China, in upper respiratory tract specimens (nasopharyngeal extracts, deep cough sputum, etc.) and lower respiratory tract specimens (alveoli irrigation fluid, etc.) by real time PCR systems.

hsa-mir-521 Real-Time RT-PCR Detection and U6 Calibration Kit
20-abx097601
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns
  • Shipped within 3 weeks.

PMA Real-Time PCR Bacterial Viability Kit - E. coli O157:H7 (Z3276)
31037 1kit
EUR 428
Description: Minimum order quantity: 1 unit of 1kit

Human Real-time PCR DNA Quantitation after QPCR DNA Damage Analysis Kit
DD2H 1 Kit
EUR 430

Mouse Real-time PCR DNA Quantitation after QPCR DNA Damage Analysis Kit
DD2M 1 Kit
EUR 430

Rat Real-time PCR DNA Quantitation after QPCR DNA Damage Analysis Kit
DD2R 1 Kit
EUR 430

PCR Mycoplasma Detection Kit
M034-Kit Kit
EUR 266

Human Lung Cancer PCR Primer Library
HLUCPL-I 1 set
EUR 548

Human Schwann Cell PCR Primer Library
HSCH-I 1 set
EUR 548

qPCR plate-foil universal (for Real-time PCR, Light Cycler 480)
A26979 1x100 pcs
EUR 186

Neisseria Gonorrhoeae Antibody
abx022105-02mg 0.2 mg
EUR 676
  • Shipped within 5-10 working days.

Neisseria Gonorrhoeae Antibody
abx022106-1mg 1 mg
EUR 913
  • Shipped within 5-10 working days.

Neisseria Gonorrhoeae Antibody
abx022107-02mg 0.2 mg
EUR 676
  • Shipped within 5-10 working days.

Neisseria Gonorrhoeae Antibody
abx022108-1mg 1 mg
EUR 982
  • Shipped within 5-10 working days.

Neisseria Gonorrhoeae Antibody
abx023101-1ml 1 ml
EUR 599
  • Shipped within 5-10 working days.

Neisseria gonorrhoeae Antibody
abx023851-1mg 1 mg
EUR 1066
  • Shipped within 5-10 working days.

Neisseria gonorrhoeae Antibody
abx023852-1mg 1 mg
EUR 1066
  • Shipped within 5-10 working days.

Neisseria gonorrhoeae Antibody
abx023853-1mg 1 mg
EUR 1066
  • Shipped within 5-10 working days.

Neisseria gonorrhoeae antibody
10-1329 1 mg
EUR 259
Description: Mouse monoclonal Neisseria gonorrhoeae antibody

Neisseria gonorrhoeae antibody
10-1330 1 mg
EUR 259
Description: Mouse monoclonal Neisseria gonorrhoeae antibody

Neisseria Gonorrhoeae Antibody
10-2759 500 ug
EUR 219
Description: Monoclonal Neisseria Gonorrhoeae Antibody

Neisseria Gonorrhoeae Antibody
10-2760 500 ug
EUR 219
Description: Monoclonal Neisseria Gonorrhoeae Antibody

Neisseria Gonorrhoeae Antibody
10-2761 500 ug
EUR 219
Description: Monoclonal Neisseria Gonorrhoeae Antibody

Neisseria gonorrhoeae antibody
10-N10A 500 ug
EUR 489
Description: Mouse monoclonal Neisseria gonorrhoeae antibody

Neisseria gonorrhoeae antibody
10-N10B 500 ug
EUR 489
Description: Mouse monoclonal Neisseria gonorrhoeae antibody

Neisseria gonorrhoeae antibody
10R-7778 500 ug
EUR 532
Description: Mouse monoclonal Neisseria gonorrhoeae antibody

Neisseria gonorrhoeae protein
30-1875 1 ml
EUR 535
Description: Purified Native Neisseria gonorrhoeae protein

Neisseria gonorrhoeae antibody
20C-CR2125RP 1 ml
EUR 554
Description: Rabbit polyclonal Neisseria gonorrhoeae antibody

Neisseria gonorrhoeae antibody
20-NR08 1 mg
EUR 140
Description: Rabbit polyclonal Neisseria gonorrhoeae antibody

Eztime Real - time PCRPremix 2x,Taqman
FYT105-100P 100 Preps 1.25 ml Ask for price

PMA Real-Time PCR Bacterial Viability Kit - E. coli O157:H7 (Z3276) PMAxx
31037-X 1kit
EUR 428
Description: Minimum order quantity: 1 unit of 1kit

Eztime Real - time PCRPremix 2x,SYBR Green
FYT103-100P 100 Preps 1.25 ml Ask for price

Eztime Real - time PCRPremix 2x,SYBR Green
FYT103-400P 400 Preps 1.25 ml x 4 Ask for price

Eztime Real - time PCRPremix 2x,SYBRGreen , ROX
FYT104-100P 100 Preps 1.25 ml Ask for price

Eztime Real - time PCRPremix 2x,SYBRGreen , ROX
FYT104-400P 400 Preps 1.25 ml x 4 Ask for price

Eztime Real - time PCRPremix 2x,Taqman, ROX
FYT105-400P 400 Preps 1.25 ml x 4 Ask for price

Eztime Real - time PCRPremix 2x,Taqman, ROX
FYT106-100P 100 Preps 1.25 ml Ask for price

Neisseria Gonorrhoeae Antibody (Biotin)
abx023098-1ml 1 ml
EUR 599
  • Shipped within 5-10 working days.

Neisseria Gonorrhoeae Antibody (HRP)
abx023100-1ml 1 ml
EUR 599
  • Shipped within 5-10 working days.

Neisseria gonorrhoeae antibody (FITC)
61C-CR2125M2F 100 ug
EUR 402
Description: Mouse monoclonal Neisseria gonorrhoeae antibody (FITC)

0,1ML THIN WALL TUBES 8 PER STRIP. CLEAR & REAL TIME STRIP CAPS
PCR-0108-LP-RT-C 125/pk
EUR 709
Description: PCR Plates & Tubes; PCR Strip Tubes and Strip Caps - Axygen

0,1ML THIN WALL TUBES 8 PER STRIP. WHITE & REAL TIME STRIP CAPS
PCR-0108-LP-RT-W 125/pk
EUR 770
Description: PCR Plates & Tubes; PCR Strip Tubes and Strip Caps - Axygen

Green Dye One Step qRT-PCR Master Mix For Quantitative Real Time PCR With ROX Dye
MB1004-100Reactions 100 Reactions
EUR 327

hsa-mir-34a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097657
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns
  • Shipped within 3 weeks.

hsa-mir-34b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097659
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns
  • Shipped within 3 weeks.

hsa-mir-92a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097661
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns
  • Shipped within 3 weeks.

hsa-mir-92b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097662
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns
  • Shipped within 3 weeks.

hsa-mir-93 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097663
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns
  • Shipped within 3 weeks.

hsa-mir-95 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097664
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns
  • Shipped within 3 weeks.

hsa-mir-96 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097665
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns
  • Shipped within 3 weeks.

hsa-mir-98 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097666
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns
  • Shipped within 3 weeks.

hsa-mir-99a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097667
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns
  • Shipped within 3 weeks.

hsa-mir-99b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097669
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns
  • Shipped within 3 weeks.

hsa-mir-100 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097671
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns
  • Shipped within 3 weeks.

hsa-mir-101 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097673
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns
  • Shipped within 3 weeks.

hsa-mir-103 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097675
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns
  • Shipped within 3 weeks.

hsa-mir-105 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097676
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns
  • Shipped within 3 weeks.

hsa-mir-106a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097677
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns
  • Shipped within 3 weeks.

hsa-mir-106b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097678
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns
  • Shipped within 3 weeks.

hsa-mir-107 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097679
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns
  • Shipped within 3 weeks.

hsa-mir-124 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097680
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns
  • Shipped within 3 weeks.

hsa-mir-125a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097681
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns
  • Shipped within 3 weeks.

hsa-mir-127 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097686
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns
  • Shipped within 3 weeks.

hsa-mir-129 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097689
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns
  • Shipped within 3 weeks.

hsa-mir-130a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097690
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns
  • Shipped within 3 weeks.

hsa-mir-130b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097691
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns
  • Shipped within 3 weeks.

hsa-mir-132 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097692
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns
  • Shipped within 3 weeks.

hsa-mir-133a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097693
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns
  • Shipped within 3 weeks.

hsa-mir-133b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097694
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns
  • Shipped within 3 weeks.

hsa-mir-134 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097695
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns
  • Shipped within 3 weeks.

hsa-mir-135b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097696
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns
  • Shipped within 3 weeks.

hsa-mir-137 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097699
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns
  • Shipped within 3 weeks.

hsa-mir-138 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097700
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns
  • Shipped within 3 weeks.

hsa-mir-139 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097701
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns
  • Shipped within 3 weeks.

hsa-mir-140 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097702
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns
  • Shipped within 3 weeks.

hsa-mir-141 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097703
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns
  • Shipped within 3 weeks.

hsa-mir-143 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097706
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns
  • Shipped within 3 weeks.

hsa-mir-145 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097708
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns
  • Shipped within 3 weeks.

hsa-mir-146a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097709
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns
  • Shipped within 3 weeks.

hsa-mir-149 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097711
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns
  • Shipped within 3 weeks.

hsa-mir-150 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097712
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns
  • Shipped within 3 weeks.

hsa-mir-151 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097714
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns
  • Shipped within 3 weeks.

hsa-mir-152 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097715
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns
  • Shipped within 3 weeks.

hsa-mir-153 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097716
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns
  • Shipped within 3 weeks.

hsa-mir-154 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097717
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns
  • Shipped within 3 weeks.

hsa-mir-181a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097719
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns
  • Shipped within 3 weeks.

hsa-mir-181b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097720
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns
  • Shipped within 3 weeks.

hsa-mir-181c Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097721
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns
  • Shipped within 3 weeks.

hsa-mir-181d Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097722
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns
  • Shipped within 3 weeks.

hsa-mir-182 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097723
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns
  • Shipped within 3 weeks.

hsa-mir-183 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097724
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns
  • Shipped within 3 weeks.

hsa-mir-184 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097725
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns
  • Shipped within 3 weeks.

hsa-mir-185 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097726
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns
  • Shipped within 3 weeks.

hsa-mir-190a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097728
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns
  • Shipped within 3 weeks.

hsa-mir-190b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097729
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns
  • Shipped within 3 weeks.

hsa-mir-192 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097731
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns
  • Shipped within 3 weeks.

hsa-mir-193a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097732
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns
  • Shipped within 3 weeks.

hsa-mir-193b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097733
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns
  • Shipped within 3 weeks.

hsa-mir-194 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097734
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns
  • Shipped within 3 weeks.

hsa-mir-195 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097735
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns
  • Shipped within 3 weeks.

hsa-mir-197 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097738
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns
  • Shipped within 3 weeks.

hsa-mir-198 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097739
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns
  • Shipped within 3 weeks.

hsa-mir-199a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097740
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns
  • Shipped within 3 weeks.

hsa-mir-199b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097741
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns
  • Shipped within 3 weeks.

hsa-mir-200a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097742
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns
  • Shipped within 3 weeks.

hsa-mir-200b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097743
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns
  • Shipped within 3 weeks.

hsa-mir-202 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097745
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns
  • Shipped within 3 weeks.

hsa-mir-203 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097746
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns
  • Shipped within 3 weeks.

hsa-mir-204 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097747
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns
  • Shipped within 3 weeks.

hsa-mir-205 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097748
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns
  • Shipped within 3 weeks.

hsa-mir-206 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097749
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns
  • Shipped within 3 weeks.

hsa-mir-208a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097750
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns
  • Shipped within 3 weeks.

hsa-mir-208b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097751
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns