Aprofarm Formulación Magistral Farmacológica

Prevalence data of diarrheagenic E. coli in the fecal pellets of wild rodents using culture methods and PCR assay

• Preston
• November 11, 2020November 17, 2020
• 0

Rapid adaptation and continuous performance evaluation of SARS-CoV-2 envelope gene (E-gene) real-time RT-PCR assays to support the hospital surge in test demand

 

e describe timely adaption of both published WHO E-gene protocol and commercially available LightMix Modular E-gene assay to the test platform (ABI 7900 Fast real-time analyzer and TaqMan Fast One-step Virus Master Mix) available in an accredited tertiary hospital laboratory with on-going evaluation to ensure provision of quality service within time constraint. The LightMix Modular E-gene was slightly more sensitive when compared to the WHO E-gene, both analytically and diagnostically. The assay was recommended for screening of SARS-CoV-2 infection. With the availability of technically competent staff through continuous training, provision of round the clock service is feasible despite the test is of high complexity.

 

Thermal cycling duration of the adapted LightMix E-gene and WHO E-gene is shortened by half and one hour respectively and allows the number of runs to double when 24-hour round-the-clock service is provided. Increase in testing capacity could support surges in testing demand, which is essential to control the current SARS-CoV-2 pandemic, to prevent potential overwhelming of the healthcare system and to optimize utilization of the isolation beds. This article is protected by copyright. All rights reserved.

 

Prevalence data of diarrheagenic E. coli in the fecal pellets of wild rodents using culture methods and PCR assay

 

Wild animals, such as rodents seem to be competent reservoir of bacteria-borne zoonotic diseases which disseminate in human. We investigated the presence of E. coli, Shiga toxin-producing E. coli (STEC), and Salmonella in the feces of six category wild rodent species (Apodemus agrarius, A. peninsulae, A. sylvaticus, Micromys minutus, Myodes regulus, and R. norvegicus) captured from different agricultural regions in South Korea. Among them, A. agrarius, which account for 65% of total (N = 52) individuals, are most widely distributed and abundant in various agroecosystems in South Korea. The bacterial identification was performed by cultural and molecular methods. In cultural method, the fecal cultures from 26 individuals formed colonies on E. coli-selective EMB agar media. Of them, the fecal cultures from 18 individuals also produced colonies on the Shiga toxin-producing E. coli-selective CT-SMAC agar media as well as the EMB agar media.

Rabbit Polyclonal antibody Anti-CRBN
Anti-CRBN	ImmunoStep	50 µg	EUR 349

anti-Relaxin 1
YF-PA14391	Abfrontier	50 ul	EUR 363
Description: Mouse polyclonal to Relaxin 1

anti-Relaxin 1
YF-PA14392	Abfrontier	100 ug	EUR 403
Description: Rabbit polyclonal to Relaxin 1

Relaxin-3, human recombinant
7195-10	Biovision		EUR 207

Relaxin-3, human recombinant
7195-50	Biovision		EUR 675

Relaxin 3 protein
30R-AR029	Fitzgerald	25 ug	EUR 273
Description: Purified recombinant Human Relaxin 3 protein

Anti-Relaxin 1 Antibody
STJ502816	St John's Laboratory	100 µg	EUR 476

Anti-Relaxin 1 (1H6)
YF-MA15197	Abfrontier	100 ug	EUR 363
Description: Mouse monoclonal to Relaxin 1

Recombinant Human Relaxin-3 Protein
PROTQ8WXF3-1	BosterBio	25ug	EUR 317
Description: Relaxin-3 (H3 relaxin, Insulin-like peptide-7, INSL7) is a secreted protein structurally related to insulin, which is expressed primarily in the brain and central nervous system. Relaxin-3 has been identified as the ligand for the GPCR135 receptor, previously known as “somatostatin-like” or “angiotensin-like” peptide receptor, and also binds specifically to the LGR7 receptor, previously identified as an “orphan” G protein coupled receptor. Signaling by Relaxin-3 through its target receptors is, most likely, part of a CNS processing system, activated in response to signaling by neuropeptides and other factors. Intracerebroventricular injections of Relaxin-3 have been shown to cause a significant increase of food intake and body weight in Wistar rats. Recombinant Relaxin-3 is a 5.5 kDa disulfide linked heterodimeric protein consisting of a 24 amino acid A-chain and a 27 amino acid B-chain.

Anti-Relaxin 1/RLN1 Antibody
A08367-1	BosterBio	100ug/vial	EUR 334

Anti-Relaxin Receptor 2 Antibody
A04848	BosterBio	100ul	EUR 397
Description: Rabbit Polyclonal Antibody for Relaxin Receptor 2 Antibody (RXFP2) detection.tested for WB in Human, Mouse, Rat.

Anti-Relaxin Receptor 2 antibody
STJ95407	St John's Laboratory	200 µl	EUR 197
Description: Rabbit polyclonal to Relaxin Receptor 2.

Anti-Relaxin Receptor 4 antibody
STJ95409	St John's Laboratory	200 µl	EUR 197
Description: Rabbit polyclonal to Relaxin Receptor 4.

Anti-Relaxin 1 Antibody BIOTIN
STJ502817	St John's Laboratory	100 µg	EUR 586

Anti-Relaxin 1 Antibody FITC
STJ502818	St John's Laboratory	100 µg	EUR 586

Anti-VEGF Receptor 3/FLT4 Antibody
A01276-3	BosterBio	100ug/vial	EUR 334

Relaxin 3 (RLN3) Antibody
20-abx218313	Abbexa	EUR 425.00 EUR 342.00	100 ug 50 ug
Shipped within 5-10 working days.

Relaxin 3 (RLN3) Antibody
20-abx178243	Abbexa	EUR 1233.00 EUR 592.00	1 mg 200 ug
Please enquire.

Relaxin 3 (RLN3) Antibody
20-abx178244	Abbexa	EUR 1233.00 EUR 592.00	1 mg 200 ug
Please enquire.

Relaxin 3 (RLN3) Antibody
20-abx121705	Abbexa	EUR 300.00 EUR 439.00 EUR 189.00	100 ul 200 ul 30 ul
Shipped within 5-10 working days.

Relaxin 3 (RLN3) Antibody
20-abx130221	Abbexa	EUR 453.00 EUR 133.00 EUR 1302.00 EUR 620.00 EUR 342.00	100 ug 10 ug 1 mg 200 ug 50 ug
Shipped within 5-7 working days.

Relaxin 3 Blocking Peptide
20-abx161415	Abbexa	EUR 606.00 EUR 1428.00	1 mg 5 mg
Shipped within 5-10 working days.

Relaxin 3 (RLN3) Antibody
20-abx174368	Abbexa	EUR 356.00 EUR 913.00 EUR 467.00 EUR 154.00 EUR 272.00	100 ug 1 mg 200 ug 20 ug 50 ug
Shipped within 5-7 working days.

Relaxin 3 (RLN3) Antibody
20-abx323035	Abbexa	EUR 314.00 EUR 244.00	100 ug 50 ug
Shipped within 5-10 working days.

Relaxin 3 Polyclonal Antibody
ABP53672-003ml	Abbkine	0.03ml	EUR 158
Immunogen information: Synthesized peptide derived from the Internal region of human Relaxin 3 Applications tips:
Description: A polyclonal antibody for detection of Relaxin 3 from Human. This Relaxin 3 antibody is for IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the Internal region of human Relaxin 3

Relaxin 3 Polyclonal Antibody
ABP53672-01ml	Abbkine	0.1ml	EUR 289
Immunogen information: Synthesized peptide derived from the Internal region of human Relaxin 3 Applications tips:
Description: A polyclonal antibody for detection of Relaxin 3 from Human. This Relaxin 3 antibody is for IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the Internal region of human Relaxin 3

Relaxin 3 Polyclonal Antibody
ABP53672-02ml	Abbkine	0.2ml	EUR 414
Immunogen information: Synthesized peptide derived from the Internal region of human Relaxin 3 Applications tips:
Description: A polyclonal antibody for detection of Relaxin 3 from Human. This Relaxin 3 antibody is for IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the Internal region of human Relaxin 3

Relaxin 3 Polyclonal Antibody
ES4671-100ul	ELK Biotech	100ul	EUR 279
Description: A Rabbit Polyclonal antibody against Relaxin 3 from Human. This antibody is tested and validated for IHC, WB, ELISA

Relaxin 3 Polyclonal Antibody
ES4671-50ul	ELK Biotech	50ul	EUR 207
Description: A Rabbit Polyclonal antibody against Relaxin 3 from Human. This antibody is tested and validated for IHC, WB, ELISA

Recombinant Relaxin 3 (RLN3)
4-RPD869Ra01	Cloud-Clone	EUR 490.66 EUR 234.00 EUR 1564.96 EUR 588.32 EUR 1076.64 EUR 391.00 EUR 3762.40	100 ug 10ug 1 mg 200 ug 500 ug 50ug 5 mg
Uniprot ID: Q8BFS3 Buffer composition: PBS, pH 7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. Form: Freeze-dried powder Predicted Molecular Mass (KD): 16.2kDa Isoelectric Point: Inquire
Description: Recombinant Rat Relaxin 3 expressed in: E.coli

Anti-14-3-3 alpha + beta Rabbit Monoclonal Antibody
M02431-3	BosterBio	100ug/vial	EUR 397
Description: Rabbit Monoclonal 14-3-3 alpha + beta Antibody. Validated in Flow Cytometry, IP, IF, IHC, ICC, WB and tested in Human, Mouse, Rat.

Relaxin H2 (human)
H-6784.0200	Bachem	0.2mg	EUR 618
Description: Sum Formula: C256H408N74O74S8; CAS# [99489-94-8] net

Relaxin H2 (human)
H-6784.1000	Bachem	1.0mg	EUR 2378
Description: Sum Formula: C256H408N74O74S8; CAS# [99489-94-8] net

Relaxin H3 (human)
H-7322.0200	Bachem	0.2mg	EUR 618
Description: Sum Formula: C237H374N70O69S6; CAS# [1158181-62-4] net

Relaxin H3 (human)
H-7322.1000	Bachem	1.0mg	EUR 2378
Description: Sum Formula: C237H374N70O69S6; CAS# [1158181-62-4] net

Anti-Human Ki67 Antibody
M00254-3	BosterBio	100ul	EUR 397
Description: Rabbit Polyclonal Human Ki67 Antibody. Validated in IHC, WB and tested in Human, Mouse, Rat.

Anti-Human IBA1 Antibody
M01394-3	BosterBio	100ul	EUR 397
Description: Chicken Polyclonal Human IBA1 Antibody. Validated in IF, IHC, WB and tested in Human.

Human Relaxin 3 (RLN3) ELISA Kit
abx253713-96tests	Abbexa	96 tests	EUR 754
Shipped within 5-12 working days.

ELISA kit for Human Relaxin 3
EK5653	SAB	96 tests	EUR 553
Description: Enzyme-linked immunosorbent assay kit for quantification of Human Relaxin 3 in samples from serum, plasma, tissue homogenates and other biological fluids.

Human Relaxin 3 PicoKine ELISA Kit
EK1407	BosterBio	96 wells	EUR 425
Description: For quantitative detection of human Relaxin 3 in cell culture supernates, serum and plasma( heparin).

Human RLN3/ Relaxin-3 ELISA Kit
E2158Hu	Sunlong	1 Kit	EUR 571

Human RLN3(Relaxin-3) ELISA Kit
EH0755	FN Test	96T	EUR 567.6
Detection range: 0.156-10 ng/ml Uniprot ID: Q8WXF3 Alias: RLN3/H3/INSL7/M3/RXN3/ZINS4/H3/insl7/relaxin 3/ZINS4/Insulin-like peptide INSL7/Insulin-like peptide 7/Prorelaxin H3/
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 0.094 ng/ml

Human Relaxin 3 (RLN3) ELISA Kit
abx512123-96tests	Abbexa	96 tests	EUR 754
Shipped within 5-12 working days.

RLN3 Relaxin-3 Human Recombinant Protein
PROTQ8WXF3	BosterBio	Regular: 25ug	EUR 317
Description: Relaxin-3 Human Recombinant produced in E.Coli is a disulfide-linked heterodimeric, non-glycosylated, polypeptide chain containing 24 amino acids for A chain and 27 amino acids for B chain and having a molecular mass of 2.5kDa for A chain and 3kDa for B chain.;The Relaxin-3 is purified by proprietary chromatographic techniques.

Human Relaxin 3(RLN3)ELISA Kit
QY-E01376	Qayee Biotechnology	96T	EUR 361

Anti-active Caspase-3 Rabbit Monoclonal Antibody
M00334-3	BosterBio	100ug/vial	EUR 397
Description: Anti-active Caspase-3 Rabbit Monoclonal Antibody tested for IF, IHC, ICC, WB in Human

Anti-Galectin 3/LGALS3 Antibody (monoclonal, 12B12)
M00621-3	BosterBio	100ug/vial	EUR 334

Polyclonal Goat anti-GST α-form
GST-ANTI-1	Detroit R&D	50 uL	EUR 280

Polyclonal Goat anti-GST μ-form
GST-ANTI-2	Detroit R&D	50 uL	EUR 280

Anti-Glyceraldehyde 3-Phosphate Dehydrogenase [GAPDH] Monoclonal Antibody
M00227-3	BosterBio	100ul	EUR 397
Description: Mouse Monoclonal Glyceraldehyde 3-Phosphate Dehydrogenase [GAPDH] Antibody. Validated in IHC, WB and tested in Equine.

Relaxin 3 receptor 1 Antibody
48026-100ul	SAB	100ul	EUR 333

Relaxin 3 receptor 1 Antibody
48026-50ul	SAB	50ul	EUR 239

Relaxin 3 receptor 1 Antibody
abx019161-100ug	Abbexa	100 ug	EUR 342
Shipped within 5-10 working days.

Rat Relaxin 3 (RLN3) Protein
20-abx168490	Abbexa	EUR 690.00 EUR 286.00 EUR 2110.00 EUR 815.00 EUR 495.00	100 ug 10 ug 1 mg 200 ug 50 ug
Shipped within 5-7 working days.

Mouse Relaxin 3 (RLN3) Protein
20-abx654935	Abbexa	EUR 578.00 EUR 258.00 EUR 1720.00 EUR 690.00 EUR 425.00	100 ug 10 ug 1 mg 200 ug 50 ug
Shipped within 5-15 working days.

Mouse Relaxin 3 (RLN3) Protein
20-abx654936	Abbexa	EUR 578.00 EUR 258.00 EUR 1720.00 EUR 690.00 EUR 425.00	100 ug 10 ug 1 mg 200 ug 50 ug
Shipped within 5-15 working days.

Relaxin Receptor 3 Polyclonal Antibody
ABP55514-003ml	Abbkine	0.03ml	EUR 158
Immunogen information: Synthesized peptide derived from the Internal region of human Relaxin Receptor 3 at AA range: 130-210 Applications tips:
Description: A polyclonal antibody for detection of Relaxin Receptor 3 from Human, Mouse, Rat. This Relaxin Receptor 3 antibody is for WB, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the Internal region of human Relaxin Receptor 3 at AA range: 130-210

Relaxin Receptor 3 Polyclonal Antibody
ABP55514-01ml	Abbkine	0.1ml	EUR 289
Immunogen information: Synthesized peptide derived from the Internal region of human Relaxin Receptor 3 at AA range: 130-210 Applications tips:
Description: A polyclonal antibody for detection of Relaxin Receptor 3 from Human, Mouse, Rat. This Relaxin Receptor 3 antibody is for WB, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the Internal region of human Relaxin Receptor 3 at AA range: 130-210

Relaxin Receptor 3 Polyclonal Antibody
ABP55514-02ml	Abbkine	0.2ml	EUR 414
Immunogen information: Synthesized peptide derived from the Internal region of human Relaxin Receptor 3 at AA range: 130-210 Applications tips:
Description: A polyclonal antibody for detection of Relaxin Receptor 3 from Human, Mouse, Rat. This Relaxin Receptor 3 antibody is for WB, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the Internal region of human Relaxin Receptor 3 at AA range: 130-210

Relaxin Receptor 3 Polyclonal Antibody
ES6513-100ul	ELK Biotech	100ul	EUR 279
Description: A Rabbit Polyclonal antibody against Relaxin Receptor 3 from Human/Mouse/Rat. This antibody is tested and validated for WB, ELISA, WB, ELISA

Relaxin Receptor 3 Polyclonal Antibody
ES6513-50ul	ELK Biotech	50ul	EUR 207
Description: A Rabbit Polyclonal antibody against Relaxin Receptor 3 from Human/Mouse/Rat. This antibody is tested and validated for WB, ELISA, WB, ELISA

ELISA kit for Human RLN3 (Relaxin-3)
E-EL-H1652	Elabscience Biotech	1 plate of 96 wells	EUR 534
Gentaur's RLN3 ELISA kit utilizes the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human RLN3. Standards or samples are added to the micro ELISA plate wells and combined with th Show more
Description: A sandwich ELISA kit for quantitative measurement of Human RLN3 (Relaxin-3) in samples from Serum, Plasma, Cell supernatant

ELISA kit for Human Relaxin-3 (RLN3)
KTE60790-48T	Abbkine	48T	EUR 332
Relaxins are known endocrine and autocrine/paracrine hormones, belonging to the insulin gene superfamily. In the human there are three non-allelic relaxin genes, RLN1, RLN2 and RLN3. RLN1 and RLN2 share high sequence homology. Relaxin is produced by Show more
Description: Quantitative sandwich ELISA for measuring Human Relaxin-3 (RLN3) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

ELISA kit for Human Relaxin-3 (RLN3)
KTE60790-5platesof96wells	Abbkine	5 plates of 96 wells	EUR 2115
Relaxins are known endocrine and autocrine/paracrine hormones, belonging to the insulin gene superfamily. In the human there are three non-allelic relaxin genes, RLN1, RLN2 and RLN3. RLN1 and RLN2 share high sequence homology. Relaxin is produced by Show more
Description: Quantitative sandwich ELISA for measuring Human Relaxin-3 (RLN3) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

ELISA kit for Human Relaxin-3 (RLN3)
KTE60790-96T	Abbkine	96T	EUR 539
Relaxins are known endocrine and autocrine/paracrine hormones, belonging to the insulin gene superfamily. In the human there are three non-allelic relaxin genes, RLN1, RLN2 and RLN3. RLN1 and RLN2 share high sequence homology. Relaxin is produced by Show more
Description: Quantitative sandwich ELISA for measuring Human Relaxin-3 (RLN3) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

Human Relaxin ELISA kit
E01R0008-192T	B-Gene	192 tests	EUR 1270
Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT Show more
Description: A competitive ELISA for quantitative measurement of Human Relaxin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Relaxin ELISA kit
E01R0008-48	B-Gene	1 plate of 48 wells	EUR 520
Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT Show more
Description: A competitive ELISA for quantitative measurement of Human Relaxin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Relaxin ELISA kit
E01R0008-96	B-Gene	1 plate of 96 wells	EUR 685
Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT Show more
Description: A competitive ELISA for quantitative measurement of Human Relaxin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Relaxin-2, human recombinant
7194-10	Biovision		EUR 207

Relaxin-2, human recombinant
7194-50	Biovision		EUR 675

Human Relaxin ELISA Kit
ELA-E1216h	Lifescience Market	96 Tests	EUR 824

Human Relaxin (RLN) Protein
20-abx654931	Abbexa	EUR 578.00 EUR 258.00 EUR 1720.00 EUR 690.00 EUR 425.00	100 ug 10 ug 1 mg 200 ug 50 ug
Shipped within 5-15 working days.

Anti-MRP1 (human) Monoclonal Antibody (QCRL-1)
M00872-3	BosterBio	1ml	EUR 675
Description: Mouse Monoclonal MRP1 (human) Antibody (QCRL-1). Validated in IP, IF and tested in Human.

Porcine relaxin peptides / relaxin (RLN) ELISA Kit
QY-E40036	Qayee Biotechnology	96T	EUR 400

Anti-Human IgM Rabbit Monoclonal Antibody, Clone#RM121
M07469-3	BosterBio	100ug	EUR 375
Description: Anti-Human IgM Rabbit Monoclonal Antibody, Clone#RM121 tested in ICC, IHC, FC, ELISA, reactive to Human

Anti-RAB3GAP2 Antibody
A07244-3	BosterBio	100ug/vial	EUR 334

Anti-FOXI1 Antibody
A07512-3	BosterBio	100ug/vial	EUR 334

Anti-IL17B Antibody
A10846-3	BosterBio	100ug/vial	EUR 294

Anti-RBMS3 Antibody
A12416-3	BosterBio	100ug/vial	EUR 334

Anti-SENP6 Antibody
A05088-3	BosterBio	100ug/vial	EUR 294

Anti-LBP Antibody
A00809-3	BosterBio	100ug/vial	EUR 294

Anti-IL22 Antibody
A00963-3	BosterBio	100ug/vial	EUR 294

Anti-MBL2 Antibody
A01000-3	BosterBio	100ug/vial	EUR 294

Anti-KCNH1 Antibody
A01036-3	BosterBio	100ug/vial	EUR 294

Anti-IL12B Antibody
A01152-3	BosterBio	100ug/vial	EUR 334

Anti-PRMT1 Antibody
A01417-3	BosterBio	100ug/vial	EUR 294

Anti-TFF3 Antibody
A01738-3	BosterBio	100ug/vial	EUR 334

Anti-IFNAR2 Antibody
A02056-3	BosterBio	100ug/vial	EUR 334

Anti-IL17F Antibody
A02062-3	BosterBio	100ug/vial	EUR 294

Anti-TREM1 Antibody
A02135-3	BosterBio	100ug/vial	EUR 294

Anti-SENP1 Antibody
A02156-3	BosterBio	100ug/vial	EUR 294

Anti-BTC Antibody
A02171-3	BosterBio	100ug/vial	EUR 334

Anti-EEA1 Antibody
A02296-3	BosterBio	100ug/vial	EUR 294

Anti-JAK3 Antibody
A02598-3	BosterBio	100ug/vial	EUR 294

Anti-APRT Antibody
A02721-3	BosterBio	100ug/vial	EUR 334

Anti-CUL2 Antibody
A02986-3	BosterBio	100ug/vial	EUR 334

Anti-EIF4A1 Antibody
A03922-3	BosterBio	100ug/vial	EUR 334

 

In molecular method, polymerase chain reaction (PCR) was carried out to detect two virulence genes (stx1 and stx2) of isolated E. coli. The amplified dataset of stx1 and stx2 genes of E. coli were sequenced. In this manuscript, E. coli and STEC were detected but there were no Salmonella species. The wild rodents’ data would provide important information on reservoirs of those pathogenic bacteria.

 

PCR Technology to Identify Minimal Residual Disease

 

Although new techniques (i.e., droplet digital-PCR, next-generation sequencing, advanced flow cytometry) are being developed, DNA-based allele-specific real-time quantitative (RQ)-PCR is still the gold standard for sensitive and accurate immunoglobulin/T cell receptor (IG/TR)-based minimal residual disease (MRD) monitoring, allowing the detection of up to 1 leukemic cell in 100,000 normal lymphoid cells. We herewith describe the standard PCR procedure which has been developed and standardized (with minor modification in single labs) through the last 20 years of activity of the EuroMRD Consortium, a volunteer activity of expert laboratories that is continuously providing education, standardization, quality control rounds, and guidelines for interpretation of RQ-PCR data.

 

Genomic Inverse PCR for Screening of Preleukemic Cells in Newborns (GIPFEL Technology)

 

Recurrent chromosomal translocations define genetic subtypes of childhood leukemia and present the first hit that generates an expanded clone of preleukemic cells in the bone marrow. Most commonly, reverse transcriptase PCR is used to detect these translocations on RNA level. This technique has severe drawbacks, including sensitivity to contamination and instability of RNA. Here, we describe the genomic inverse PCR for exploration of ligated breakpoints (GIPFEL) that overcomes these pitfalls.